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Ultrasound attenuation imaging: a reproducible alternative for the noninvasive quantitative assessment of hepatic steatosis in children

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Abstract

Background

Pediatric hepatic steatosis is a global public health concern, as an increasing number of children are affected by this condition. Liver biopsy is the gold standard diagnostic method; however, this procedure is invasive. Magnetic resonance imaging (MRI)-derived proton density fat fraction has been accepted as an alternative to biopsy. However, this method is limited by cost and availability. Ultrasound (US) attenuation imaging is an upcoming tool for noninvasive quantitative assessment of hepatic steatosis in children. A limited number of publications have focused on US attenuation imaging and the stages of hepatic steatosis in children.

Objective

To analyze the usefulness of ultrasound attenuation imaging for the diagnosis and quantification of hepatic steatosis in children.

Material and methods

Between July and November 2021, 174 patients were included and divided into two groups: group 1, patients with risk factors for steatosis (n = 147), and group 2, patients without risk factors for steatosis (n = 27). In all cases, age, sex, weight, body mass index (BMI), and BMI percentile were determined. B-mode US (two observers) and US attenuation imaging with attenuation coefficient acquisition (two independent sessions, two different observers) were performed in both groups. Steatosis was classified into four grades (0: absent, 1: mild, 2: moderate and 3: severe) using B-mode US. Attenuation coefficient acquisition was correlated with steatosis score according to Spearman’s correlation. Attenuation coefficient acquisition measurements’ interobserver agreement was assessed using intraclass correlation coefficients (ICC).

Results

All attenuation coefficient acquisition measurements were satisfactory without technical failures. The median values for group 1 for the first session were 0.64 (0.57–0.69) dB/cm/MHz and 0.64 (0.60–0.70) dB/cm/MHz for the second session. The median values for group 2 for the first session were 0.54 (0.51–0.56) dB/cm/MHz and 0.54 (0.51–0.56) dB/cm/MHz for the second. The average attenuation coefficient acquisition was 0.65 (0.59–0.69) dB/cm/MHz for group 1 and 0.54 (0.52–0.56) dB/cm/MHz for group 2. There was excellent interobserver agreement at 0.94 (95% CI 0.92–0.96). There was substantial agreement between both observers (κ = 0.77, with a P < 0.001). There was a positive correlation between ultrasound attenuation imaging and B-mode scores for both observers (r = 0.87, P < 0.001 for observer 1; r = 0.86, P < 0.001 for observer 2). Attenuation coefficient acquisition median values were significantly different for each steatosis grade (P < 0.001). In the assessment of steatosis by B-mode US, the agreement between the two observers was moderate (κ = 0.49 and κ = 0.55, respectively, with a P < 0.001 in both cases).

Conclusion

US attenuation imaging is a promising tool for the diagnosis and follow-up of pediatric steatosis, which provides a more repeatable form of classification, especially at low levels of steatosis detectable in B-mode US.

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Authors and Affiliations

  1. Department of Radiology, Hospital de Pediatría Dr. Juan P. Garrahan, Combate de los Pozos 1881 (C 1245 AAM), Buenos Aires, Argentina

    Esteban P. Dardanelli, María Eugenia Orozco, Vanesa Oliva, Juan Francisco Lutereau, Facundo Agustín Ferrari, Mónica G. Bravo & Julio S. Kaplan

  2. Department of Research and Development, Hospital de Pediatría Dr. Juan P. Garrahan, Combate de los Pozos 1881 (C 1245 AAM), Buenos Aires, Argentina

    Silvina Ruvinsky & Macarena Roel

  3. Department of Pediatrics, Hospital de Pediatría Dr. Juan P. Garrahan, Combate de los Pozos 1881, Buenos Aires, Argentina

    Pablo C. Barvosa

  4. Department Nutrition, Hospital de Pediatría Dr. Juan P. Garrahan, Combate de los Pozos 1881, Buenos Aires, Argentina

    Marisa Armeno

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  1. Esteban P. Dardanelli
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Correspondence to Esteban P. Dardanelli.

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Approval was obtained from the ethics committee of Garrahan Hospital. The procedures used in this study adhere to the tenets of the Declaration of Helsinki.

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Informed consent was obtained from all individual participants included in this study.

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Dardanelli, E.P., Orozco, M.E., Oliva, V. et al. Ultrasound attenuation imaging: a reproducible alternative for the noninvasive quantitative assessment of hepatic steatosis in children. Pediatr Radiol 53, 1618–1628 (2023). https://doi.org/10.1007/s00247-023-05601-0

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