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Gold nanoparticle-decorated fluorine-doped tin oxide substrate for sensitive label-free OIRD microarray chips

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Abstract

Oblique incidence reflectance difference (OIRD) is an emerging technique enabling real-time and label-free detection of bio-affinity binding events on microarrays. The interfacial architecture of the microarray chip is critical to the performance of OIRD detection. In this work, a sensitive label-free OIRD microarray chip was developed by using gold nanoparticle-decorated fluorine-doped tin oxide (AuNPs-FTO) slides as a chip substrate. This AuNPs-FTO chip demonstrates a higher signal-to-noise ratio and improved sensitivity compared to that built on FTO glass, showing a detection limit of as low as 10 ng mL−1 for the model target, HRP-conjugated streptavidin. On-chip ELISA experiments and optical calculations suggest that the enhanced performance is not only due to the higher probe density enabling a high capture efficiency toward the target, but most importantly, the AuNP layer arouses optical interference to improve the intrinsic sensitivity of OIRD. This work provides an effective strategy for constructing OIRD-based microarray chips with enhanced sensitivity, and may help extend their practical applications in various fields.

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Funding

This work received financial support from National Natural Science Foundation of China (No. 22074125), Natural Science Foundation Project of CQ CSTC (cstc2021jcyj-msxmX1165), and The Innovation Platform for Academicians of Hainan Province.

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Authors and Affiliations

  1. Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Materials and Energy, Southwest University, Chongqing, 400715, People’s Republic of China

    Yuda Ren, Meng Li, **aoyi Li, Jun Ye, Zhihao Feng & Weihua Hu

  2. Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, Key Laboratory of Functional Materials and Photoelectrochemistry of Haikou, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, People’s Republic of China

    Wei Sun

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  1. Yuda Ren
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  2. Meng Li
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  5. Zhihao Feng
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  6. Wei Sun
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Contributions

Yuda Ren: methodology; formal analysis; investigation; writing, original draft preparation. Meng Li: formal analysis and software. **aoyi Li: conceptualization; methodology. Jun Ye: investigation; project administration. Zhihao Feng: formal analysis; investigation; software. Wei Sun: conceptualization; funding acquisition; writing, review and editing. Weihua Hu: conceptualization; methodology; funding acquisition; writing, review and editing. All authors read and approved the final manuscript.

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Ren, Y., Li, M., Li, X. et al. Gold nanoparticle-decorated fluorine-doped tin oxide substrate for sensitive label-free OIRD microarray chips. Anal Bioanal Chem 416, 3775–3783 (2024). https://doi.org/10.1007/s00216-024-05318-5

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