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Fluorescent-protein-based probes: general principles and practices

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Abstract

An important application of fluorescent proteins is to derive genetically encoded fluorescent probes that can actively respond to cellular dynamics such as pH change, redox signaling, calcium oscillation, enzyme activities, and membrane potential. Despite the large diverse group of fluorescent-protein-based probes, a few basic principles have been established and are shared by most of these probes. In this article, the focus is on these general principles and strategies that guide the development of fluorescent-protein-based probes. A few examples are provided in each category to illustrate the corresponding principles. Since these principles are quite straightforward, others may adapt them to create fluorescent probes for their own interest. Hopefully, the development of the ever-growing family of fluorescent-protein-based probes will no longer be limited to a small number of laboratories specialized in senor development, leading to the situation that biological studies will be bettered assisted by genetically encoded sensors.

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Acknowledgments

I acknowledge support from the University of California, Riverside and the National Science Foundation for grant CHE-1351933.

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  1. Department of Chemistry, University of California Riverside, Riverside, CA, 92521, USA

    Hui-wang Ai

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  1. Hui-wang Ai
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Correspondence to Hui-wang Ai.

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Ai, Hw. Fluorescent-protein-based probes: general principles and practices. Anal Bioanal Chem 407, 9–15 (2015). https://doi.org/10.1007/s00216-014-8236-3

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  • DOI: https://doi.org/10.1007/s00216-014-8236-3

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