Abstract
This study describes phycocompounds of the non-N2-fixing filamentous cyanobacterium Lyngbya sp., which has potential bio-reducing and stabilizing heavy metal–accumulating properties for synthesizing silver nanoparticles (AgNPs), whose formation was confirmed by the colour change of the Lyngbya sp.-AgNP solution from pale green to deep brown. The reduction of ‘Lyngbya sp.-AgNPs’, called Lsp-AgNPs, was proved by UV-visible photo-spectrometry analysis with an obtained peak value at 426 nm. Lsp-AgNPs were characterised by analytical techniques, XRD, FESEM, DLS and FTIR. The XRD analysis with 5–70 theta was obtained at 2ϴ angles ranging from 38.79º with intensity, indicating the crystal structure of Lsp-AgNPs. The FESEM analysis indicated the area size at 20–50 µm; in the DLS analysis, the peak at 400 d nm indicated the size and distribution of Lsp-AgNPs. In FTIR analysis, the peaks were obtained at wavenumbers 3338, 1639, and 542 cm−1, which indicated the presence of N–H, –OH and C=O functional groups in Lsp-AgNPs. Those had in vitro antibacterial activities against Gram-negative Escherichia coli (MTCC 443) and Pseudomonas aeruginosa (MTCC 1688) and Gram-positive Staphylococcus aureus (MTCC 7443) bacterial strains with zone of inhibitions (ZOI) of 16, 12 and 14 mm, respectively, with comparing the antibiotic gentamycin as a positive control, as was monitored with agar-well diffusion method. Furthermore, the MIC value was 50 mg/ml, and MBC values of 65 mg/ml of Lsp-AgNPs were effective against those bacteria. Thus, Lsp-AgNPs had potential antibacterial activities against MDR pathogenic S. aureus, E. coli and P. aeruginosa. In conclusion, MDR pathogenic bacteria could be controlled as prodrugs in the future.
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The datasets used and analysed during the current study are available from the corresponding authors upon reasonable request.
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Acknowledgements
The authors thank Prof. Dr. S. Mishra, Dean, Institute of Medical Sciences and Sum Hospital, Bhubaneswar, Odisha, for encouragement.
Funding
This work, along with S. Swain, received funding through a PhD fellowship (University Registration Number 2181611011/2021) in Biotechnology from SOA Deemed to be University in Bhubaneswar, Odisha.
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SS: Writing an original work, collecting the reference materials, reviewing and editing. SB: AKB: BRJ: reviewing and editing RNP: Conceptualisation, reviewing, editing, and supervision. The authors declare that all data were generated in-house and that no paper mill was used.
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Highlights
• Biosynthesis of silver oxide with cyanobacterium Lyngbya sp.
• Spectral characterisations of biosynthesised L sp-AgNPs.
• In vitro antibacterial assessment of L sp-AgNPs.
• L sp-AgNPs may play a role as future antibacterial druggable agents.
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Swain, S., Bej, S., Bishoyi, A.K. et al. Biosynthesis and characterisations of silver nanoparticles with filamentous cyanobacterium Lyngbya sp. with in vitro antibacterial properties against MDR pathogenic bacteria. Naunyn-Schmiedeberg's Arch Pharmacol (2024). https://doi.org/10.1007/s00210-024-03235-z
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DOI: https://doi.org/10.1007/s00210-024-03235-z