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Gracilibacillus eburneus sp.nov., a moderately halophilic bacterium isolated from **njiang province, China

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Abstract

A novel Gram-staining positive, moderately halophilic, endospore-forming, motile, rod-shaped and strictly aerobic strain, designated YIM 93565T, was isolated from a salt lake in **njiang province of China and subjected to a polyphasic taxonomic study. Strain YIM 93565T grew in the range of pH 6.0–9.0 (optimum pH 7.0), 10–45 °C (optimum 35–40 °C) and at salinities of 2–24% (w/v) NaCl (optimum 7–10%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 93565T clustered with members of the genera Gracilibacillus and form a clade with Gracilibacillus bigeumensis KCTC 13130T (95.6% similarity) and Gracilibacillus halophilus DSM 17856T (94.9%), which was well separated from others. The DNA G + C content of this novel strain was 36.8 mol%. The major fatty acids were anteiso-C15:0, iso-C15:0, C16:0 and anteiso-C17:0 and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and two unidentified phospholipids. The predominant menaquinone was MK-7. The cell-wall peptidoglycan was based on meso-diaminopimelic acid. Based on the results of phylogenetic, physiological and chemotaxonomic comparative analyses, the isolate is assigned to a novel species of the genus Gracilibacillus, for which the name Gracilibacillus eburneus sp. nov. is proposed, with the type strain YIM 93565T (= DSM 23710T = CCTCC AB 2013249T).

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Acknowledgements

This work was supported by Grants from the National Natural Science Foundation of China (no. 41361075, 31160003, 31270055), the Key Research Program of Application Foundation of Yunnan Province (2013FA015), and the major special project of scientific research fund in Yunnan Education Department (ZD2013008).

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Author notes

  1. Hui-Lin Guan and Yun-Jiao Zhang contributed equally to this study.

Authors and Affiliations

  1. Education Ministry Key Laboratory of Renewable Energy Advanced Materials and Manufacturing Technology, Energy and Environment Science College, Yunnan Normal University, Kunming, 650092, People’s Republic of China

    Hui-Lin Guan, **ao-Ju Lu, Zheng-Yun Zhang & Xu-Hong Gao

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yun-Jiao Zhang, **ao-Ju Lu, Man Jia, Zheng-Yun Zhang, Yong-Chun Ma, Fei Tian & Shu-Kun Tang

  3. Kunming Medical University Haiyuan College, Kunming, 650106, People’s Republic of China

    Yun-Jiao Zhang

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  1. Hui-Lin Guan
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Correspondence to Shu-Kun Tang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank accession number for the 16S rRNA gene sequence of strain YIM 93565T is KF548095. The ‘digital protologue’ database (DPD) TaxonNumber of strain YIM 93565T is TA00291 (http://imedea.uib-csic.es/dprotologue/).

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Guan, HL., Zhang, YJ., Lu, XJ. et al. Gracilibacillus eburneus sp.nov., a moderately halophilic bacterium isolated from **njiang province, China. Arch Microbiol 200, 423–429 (2018). https://doi.org/10.1007/s00203-017-1450-6

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  • DOI: https://doi.org/10.1007/s00203-017-1450-6

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