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Functional characterization of glucosamine-6-phosphate synthase (GlmS) in Salmonella enterica serovar Enteritidis

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Abstract

Salmonella is a threat to public health due to consumption of contaminated food. Screening of a transposon library identified a unique mutant that was growth and host cell binding deficient. The objective of this study was to determine the functional role of glucosamine-6-phosphate synthase (GlmS) in the biology and pathogenesis of Salmonella. To examine this, we created a glmS mutant (ΔglmS) of Salmonella and examined the effect on cell envelope integrity, growth, metabolism, and pathogenesis. Our data indicated ΔglmS was defective in growth unless media were supplemented with d-glucosamine (d-GlcN). Examination of the bacterial cell envelope revealed that ΔglmS was highly sensitive to detergents, hydrophobic antibiotics, and bile salts compared to the wild type (WT). A release assay indicated that ΔglmS secreted higher amounts of β-lactamase than the WT in culture supernatant fractions. Furthermore, ΔglmS was attenuated in cell culture models of Salmonella infection. Taken together, this study determined an important role for GlmS in the pathogenesis and biology of Salmonella.

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Acknowledgments

This study was supported by funding from the Department of Animal Sciences and the Graduate School, University of Wisconsin-Madison.

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Authors and Affiliations

  1. Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, WI, 53706, USA

    Alexis M. Bennett, Daniel C. Shippy, Nicholas Eakley & Amin A. Fadl

  2. Wisconsin Veterinary Diagnostic Laboratory, University of Wisconsin-Madison, Madison, WI, 53706, USA

    Ogi Okwumabua

Authors
  1. Alexis M. Bennett
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  2. Daniel C. Shippy
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  3. Nicholas Eakley
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  4. Ogi Okwumabua
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  5. Amin A. Fadl
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Correspondence to Amin A. Fadl.

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Bennett, A.M., Shippy, D.C., Eakley, N. et al. Functional characterization of glucosamine-6-phosphate synthase (GlmS) in Salmonella enterica serovar Enteritidis. Arch Microbiol 198, 541–549 (2016). https://doi.org/10.1007/s00203-016-1212-x

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  • DOI: https://doi.org/10.1007/s00203-016-1212-x

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