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Approaches to characterize the transcriptional trajectory of human myogenesis

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Abstract

Human pluripotent stem cells (hPSCs) have attracted considerable interest in understanding the cellular fate determination processes and modeling a number of intractable diseases. In vitro generation of skeletal muscle tissues using hPSCs provides an essential model to identify the molecular functions and gene regulatory networks controlling the differentiation of skeletal muscle progenitor cells. Such a genetic roadmap is not only beneficial to understanding human myogenesis but also to decipher the molecular pathology of many skeletal muscle diseases. The combination of established human in vitro myogenesis protocols and newly developed molecular profiling techniques offers extensive insight into the molecular signatures for the development of normal and disease human skeletal muscle tissues. In this review, we provide a comprehensive overview of the current progress of in vitro skeletal muscle generation from hPSCs and relevant examples of the transcriptional landscape and disease-related transcriptional aberrations involving signaling pathways during the development of skeletal muscle cells.

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Acknowledgements

We thank members of the Lee lab for providing valuable discussions.

Funding

This work was supported by NIH grants R01NS093213 (GL), R01AR070751 (GL), the Maryland Stem Cell Research Fund (MSCRF) (GL), the Muscular Dystrophy Association (MDA) (GL), and the Global Research Development Center Program from the National Research Foundation of Korea (NRF) (2017K1A4A3014959, G.L. & S.-H.H.).

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Authors and Affiliations

  1. Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Republic of Korea

    HoTae Lim & Sang-Hwan Hyun

  2. School of Medicine, Institute for Cell Engineering, Johns Hopkins University, Baltimore, MD, 21205, USA

    HoTae Lim, In Young Choi, Sang-Hwan Hyun, Hyesoo Kim & Gabsang Lee

  3. Department of Pathology, Graduate School, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea

    In Young Choi

  4. Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA

    Hyesoo Kim & Gabsang Lee

  5. The Solomon H. Snyder Department of Neuroscience, School of Medicine, Johns Hopkins University, Baltimore, MD, 21205, USA

    Gabsang Lee

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  1. HoTae Lim
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  2. In Young Choi
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  3. Sang-Hwan Hyun
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  4. Hyesoo Kim
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HTL analyzed the published data and wrote the manuscript. IYC wrote the manuscript. HK wrote the manuscript. SHH over-saw data interpretation and contributed to writing the manuscript. GL designed the review paper and wrote the manuscript.

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Correspondence to Gabsang Lee.

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GL is a founder of the Vita Therapeutics. IYC, HTL and GL are shareholders of the Vita Therapeutics. SHH and HK declare no potential conflict of interest.

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Lim, H., Choi, I.Y., Hyun, SH. et al. Approaches to characterize the transcriptional trajectory of human myogenesis. Cell. Mol. Life Sci. 78, 4221–4234 (2021). https://doi.org/10.1007/s00018-021-03782-1

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