Abstract
Genome types of 22 banana specimens (Musa species) and one species of Ensete from Arunachal Pradesh and Manipur, North Eastern India, were characterized by morphological scoring system and IRAP-PCR molecular technique. Sixteen specimens were identified up to species levels, and six were identified as cultivars/hybrids of Musa acuminata (AA) and M. balbisiana (BB). Seven Musa specimens were classified as members of the ornamental banana under the section Rhodochlamys, whereas 15 were members of the section Eumusa. Score card based characterization revealed five genome types of which majority of the banana specimens belongs to allopolyploids (19) while only four were autopolyploids. Allotriploid genomes (AAB/ABB) were the largest group recorded in 15 specimens while, autopolyploid genomes (AA/AAA and BB/BBB) were found in four specimens. Genome distribution varied from highest of 45% in AAB (Dessert banana) followed by 23% ABB (Cooking banana), 17% AB (Ney Poovan), and 9% each for AA/AAA (Cavendish group) and BB/BBB (Saba group), respectively. The genome type of Ensete glaucum was found to be allodiploid (AB). Molecular characterization using IRAP-PCR profiles revealed the presence of B genome specific band (~350 bp) in 21 specimens except two specimens of M. acuminata. This band is considered to be a characteristic signature of M. balbisiana genome type (BB). The brightness intensities of the B specific band were significantly high in M. balbisiana as compared to other specimens. A larger size band of ~430 bp and another small sized band of ~250 bp were observed in the two specimens of M. acuminata as well as in all other specimens identified to have “A” genome except M. balbisiana. Based on this study, presence of ~430 bp DNA band in IRAP profiles of banana can be considered as a signature band for identification of A genome in wild and cultivated bananas. The present study concludes that morphological scoring system could classify genome types with their economic values while IRAP-PCR based molecular characterization can validate presence or absence of A or B genome types in wild species and cultivated varieties of banana.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Cheesman EE (1947) Classification of the bananas. I. The genus Ensete Horan and the genus Musa L. Kew Bull 2:97–117
Cheesman EE (1950) Classification of the bananas III. Critical notes on species. Kew Bull 5(27–31):151–155
Christelova P, Valarik M, Hribova E et al (2011) A platform for efficient genoty** in Musa using microsatellite markers. AoB Plants Special issue plr024-p. 1–14
Daniells J, Jenny C, Karamuara CD et al (2001) Musalogue: a catalogue on Musa germplasm. Diversity in the genus Musa. INIBAP, Montpelier, France, p 213
De Langhe E, Pillay M, Tenkouano A et al (2005) Integrating morphological and molecular taxonomy in Musa: the Africa plantains (Musa spp. AAB group). Plant Syst Evol 255:225–236
FAO (2021) Banana facts and figures. Food and Agricultural Organization of the United Nations. http://www.fao.org/economic/est/estcommodities/bananas/bananafacts/en/#.YIxNSLUzaUk. Accessed 19 Feb 2021
Hakkinen M (2013) Reappraisal of sectional taxonomy in Musa (Musaceae). Taxon 62(4):809–813
Hakkinen M, Vare H (2008) Typification and check-list of Musa L. names (Musaceae) with nomenclatural notes. Adansonia 30(1):63–112
Heslop-Harrison JS, Schwarzacher T (2007) Domestication, genomics and the future of the banana. Ann Bot 100:1073–1084
IPGRI-INIBAP-CIRAD (1996) Descriptors for Banana (Musa spp.) pp55. https://www.bioversityinternational.org/e-library/publications/detail/descriptors-for-banana-musa-spp/
MUSANEI (2020) MUSANEI—An image based digital database on banana (Musa spp.) resources of North Eastern India. Accessed online on 04/05/2021 (https://musanei.in)
Nair SA, Toe HC, Schwarzacher T et al (2005) Genome clarification of banana cultivars from South India using IRAP. Euphytica 144(3):258–290
Ning SP, Xu LB, Lu Y et al (2007) Genome composition and genetic diversity of Musa germplasm from China revealed by PCR-RFLP and SSR markers. Sci Hortic 114:281–288
Nwakanma DC, Pillay M, Okoli BE et al (2003) PCR-RFLP of the ribosomal DNA internal transcribed spacers (ITS) provides markers for the A and B genomes in Musa L. Theor Appl Genet 108:154–159
Pachuau L, Atom AD, Thangjam R (2014) Genome classification of Musa cultivars from Northeast India as revealed by ITS and IRAP markers. Appl Biochem Biotechnol:17. https://doi.org/10.1007/s12010-014-0827-0
Pillay M, Nwakanma DC, Tenkouano A (2000) Identification of RAPD markers linked to a and B genome sequences in Musa L. Genome 43:763–767
Robinson JC, Galán Saúco V (2010) Banana and Plantains, CAB International, pp 311
Rogers SO, Bendich AJ (1994) Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual, 2nd edn. Kluwer Academic Publishers, Dordrecht, pp 1–8
Shelke RG, Das AB (2015) Analysis of genetic diversity in 21 genotypes of Indian banana using RAPDs and IRAPs markers. Proc Natl Acad Sci, Sect B Biol Sci 85(4):1027–1038
Silayoi B, Chomchalow N (1987) Cytotaxonomic and morphological studies of Tahi Banana cultivars. In: Parsley GJ, De Langhe E (eds) Banana and plantain breeding strategies, ACIAR proceeding no. 21, pp 157–160
Simmond NW (1962) The evolution of the bananas. Longmans, London, p 170
Simmonds NW, Shepherd K (1955) The taxonomy and origins of cultivated bananas. Bot J Linn Soc 55:320–312
Singh HP, Uma S (1996) Genetic diversity of banana in India. In: The proceedings of the conference on “Challenges for banana Production and utilization in 21st century” held at Trichy, Sept 24–25
Singh WA, Singh NS, Handique PJ, Devi HS (2014) Morpho-taxonomical and molecular assessment of Musa genotypes from North-East India by morphological and inter-retrotransposon amplified polymorphism markers. Plant Syst Evol 301:563–575
Ude G, Pillay M, Ogundiwin E et al (2002) Analysis of genetic diversity and sectional relationships in Musa using AFLP markers. Theor Appl Genet 104:1239–1245
Uma S, Sathiamoorthy S (2002) Names and synonyms of bananas and plantains of India, National Research Centre for Banana (ICAR), Tiruchirapalli, India
Uma S, Shyam B, Selvaranjan R et al (2001) Collection and Characterization of Banana and Plantain of Northeastern India: Advancing Banana and plantain R & D in Asia and Pacific- vol. 10 Proceeding of the 10th INIBAP – SPNET – Regional Advisory Committee meeting held at Bangkok, Thailand. November 2000. pp. 103–109
Uma S, Siva SA, Saraswathi MS et al (2006) Variation and intraspecific relationships in India wild Musa balbisiana (BB) population as evidenced by random amplified polymorphic DNA. Genet Resour Crop Evol 53:349–355
Valentini A, Pompanon F, Taberlet P (2009) DNA barcoding for ecologists. Trends Ecol Evol 24:110–117
Conflict of Interest
The authors declare that they have no conflict of interest.
Acknowledgments
Authors gratefully acknowledge the Department of Biotechnology, Government of India sponsored Bioinformatics Infrastructure Facility (DBT-BIF), Department of Forestry for providing research facilities and studentship during the study.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Ranibala, G., Sureshkumar Singh, S., Khan, M.L. (2022). Morphological and Molecular Characterization of Genome Types in Wild and Cultivated Bananas (Musa Species) of Two States in North Eastern India. In: Kumar, A., Choudhury, B., Dayanandan, S., Khan, M.L. (eds) Molecular Genetics and Genomics Tools in Biodiversity Conservation. Springer, Singapore. https://doi.org/10.1007/978-981-16-6005-4_14
Download citation
DOI: https://doi.org/10.1007/978-981-16-6005-4_14
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-16-6004-7
Online ISBN: 978-981-16-6005-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)