Abstract
Limited source of fossil fuel, increasing global need of energy, and environmental concerns due to the use of fossil fuels, such as global warming, pollution, and ozone layer depletion, led to demand for a non-conventional and sustainable source of energy. Biofuels are one such source of energy that are produced from various biological sources. Currently, biofuels are mainly produced from sugars and vegetable oil from sugarcane, corn, soybean, palm, etc. and are catagorized as first-generation biofuels. Considering that these feedstocks are also used for human consumption, the focus has now shifted on second-generation biofuels where non-edible plants and agricultural residues are explored for biofuel production. However, due to the complexity of the feedstock involved, second-generation biofuels need extensive research and development before it can be treated as economically viable. Similarly, third-generation biofuels that are produced from microalgae also need much innovation before it can be commercially realized. In recent years, the rapid development in the field of metabolic engineering and synthetic biology for microorganisms like bacteria, yeast, cyanobacteria, fungi, and algae provides an immense scope for using these techniques and methods for engineering the microbes for higher production of various fuel molecules. In this chapter, we discuss the advancement in the area of metabolic engineering and synthetic biology, along with their applications in enhanced production of biofuels from different microorganisms.
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Kumar, R., Nair, A.M., Yazdani, S.S. (2022). Role of Metabolic Engineering and Synthetic Biology in the Development of Microbes for Biofuel Production. In: Saini, J.K., Sani, R.K. (eds) Microbial Biotechnology for Renewable and Sustainable Energy. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-16-3852-7_9
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