Abstract
TCA cycle is widely present among all heterotrophs growing aerobically. It operates throughout in the presence of oxygen. However, in some facultative microorganisms, it runs partially with activation of some new enzyme activities and also down-regulation of existing enzymes under anaerobic or microaerophilic conditions. In such conditions, the TCA cycle is branched. Various enzyme activities which are up-regulated or down-regulated are discussed. Further, reductant generation and ATP yields under microaerophilic conditions are also discussed. When Escherichia coli is grown on acetate (2-carbon compound) as a source of carbon, glyoxylate pathway operates, which is a carbon conservation pathway. Details of glyoxylate pathway and its regulation are dealt with. Pathway engineering for over-production of succinic acid is discussed as part of a case study.
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References
Cozzone, A. J. (1998). Regulation of acetate metabolism by protein phosphorylation in enteric bacteria. Annual reviews of microbiology, 52, 127–164.
Cozzone, A. J., & El-Mansi, M. (2005). Control of isocitrate dehydrogenase catalytic activity by protein phosphorylation in Escherichia coli. Journal of Molecular Microbiology and Biotechnology, 9(3–4), 132–146.
Ensign, S. A. (2006). Revisiting the glyoxylate cycle: Alternate pathways for microbial acetate assimilation. Molecular Microbiology, 61(2), 274–276.
Garnak, M., & Reeves, H. C. (1979). Phosphorylation of Isocitrate dehydrogenase of Escherichia coli. Science, 203(4385), 1111–1112.
Gui, L., Sunnarborg, A. & LaPorte, D. C. (1996a). Regulated expression of a repressor protein: FadR activates iclR. Journal of Bacteriology, 178(15), 4704–4709.
Gui, L., Sunnarborg, A., Pan, B. & LaPorte, D. C. (1996b). Autoregulation of iclR, the gene encoding the repressor of the glyoxylate bypass operon. Journal of Bacteriology, 178(1), 321–324.
Hong, S. H. (2007). Systems approaches to succinic acid-producing microorganisms. Biotechnology and Bioprocess Engineering, 12(2), 73–79.
Kornberg, H. L. (1966). The role and control of the glyoxylate cycle in Escherichia coli. Biochemical Journal, 99(1), 1–11.
Lorca, G. L., Ezersky, A., Lunin, V. V., Walker, J. R., Altamentova, S., Evdokimova, E., et al. (2007). Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. Journal of Biological Chemistry, 282(22), 16476–16491.
Resnik, E., Pan, B., Ramani, N., Freundlich, M., & LaPorte, D. C. (1996). Integration host factor amplifies the induction of the aceBAK operon of Escherichia coli by relieving IclR repression. Journal of Bacteriology, 178(9), 2715–2717.
Sawers, M. (2005). Formate and its role in hydrogen production in Escherichia coli. Biochemical Society Transactions, 33(1), 42–46.
Song, H., & Lee, S. Y. (2006). Production of succinic acid by bacterial fermentation. Enzyme and Microbial Technology, 39(3), 352–361.
Sunnarborg, A., Klumpp, D., Chung, T., & LaPorte, D. C. (1990). Regulation of the glyoxylate bypass operon: Cloning and characterization of iclR. Journal of Bacteriology, 172(5), 2642–2649.
Zheng, J., & Jia, Z. (2010). Structure of the bifunctional isocitrate dehydrogenase kinase/phosphatase. Nature, 465(7300), 961–965.
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Gupta, R., Gupta, N. (2021). Alternate Tricarboxylic Acid Cycle. In: Fundamentals of Bacterial Physiology and Metabolism. Springer, Singapore. https://doi.org/10.1007/978-981-16-0723-3_13
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DOI: https://doi.org/10.1007/978-981-16-0723-3_13
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