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Chapter 11 Fertilization in Amphibians: The Cellular and Molecular Events from Sperm Approach to Egg Activation

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Reproduction in Aquatic Animals

Abstract

The mechanism of fertilization is important for understanding the role of sexual reproduction in animals. Amphibians are one of the most suitable systems to investigate the cellular and molecular mechanisms of fertilization. Inseminated sperm approach the egg by initiation of motility and chemotactic guidance in response to substances secreted from oviducts on the egg surface. Sperm undergoing the acrosome reaction penetrate the vitelline envelope, and then fuse with the egg plasma membrane. The fertilizing sperm induces an increase in the intracellular Ca2+ concentration, causing egg activation, and initiation of embryonic development. The activated egg elicits several blocks to polyspermy, ensuring development from only one sperm. Recent advances in cellular and molecular mechanisms in amphibian fertilization are summarized and their important roles for establishment of amphibian fertilization are discussed.

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Acknowledgements

We thank Dr. Akihiko Watanabe, Yamagata University, for reviewing the manuscript. This work was supported by JSPS KAKENHI Grant Numbers, JP16K07373 and JP19K06690 to Y.I. and by The YU “Pump-Priming Program” for Fostering Research Activities.

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  1. Laboratory of Reproductive Developmental Biology, Division of Earth Sciences, Biology, and Chemistry, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, Yamaguchi, Japan

    Yasuhiro Iwao & Mami Watabe

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  1. Yasuhiro Iwao
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  2. Mami Watabe
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Correspondence to Yasuhiro Iwao .

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  1. Misaki Marine Biological Station, The University of Tokyo, Miura, Kanagawa, Japan

    Manabu Yoshida

  2. Institute for Animal Science and Technology, Universitat Politècnica de València, Valencia, Spain

    Juan F. Asturiano

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Iwao, Y., Watabe, M. (2020). Chapter 11 Fertilization in Amphibians: The Cellular and Molecular Events from Sperm Approach to Egg Activation. In: Yoshida, M., Asturiano, J. (eds) Reproduction in Aquatic Animals. Springer, Singapore. https://doi.org/10.1007/978-981-15-2290-1_12

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