Abstract
Informal milk marketing chains provide the major milk supply link from smallholder dairy production systems to consumers in develo** countries. Prevalence risk of antibiotic residues was investigated in milk samples (n = 528) collected from different levels of informal supply chains in Pakistan from 2012–2013. After screening, all positive samples were further analyzed by High Performance Liquid Chromatography to quantify individual β-lactam residues. Fifteen percent of the total samples were found to be positive for at least one of the antibiotics. All positive samples (81/528) were positive for amoxicillin. Percentage of positive samples for ampicillin and penicillin was 12.1 and 6.4% respectively. Percentages of positive samples collected from farmers, small collectors, large collectors and retailers were 17.5, 15.1, 8.3 and 13.5 respectively. When relating to season of collection 11.3, 10.2, 19.1, 17.9 and 16.3% of samples in autumn, monsoon, spring, summer and winter were found positive. Concentrations of amoxicillin, ampicillin and penicillin in positive samples were 79.5 ± 12.15, 106.6 ± 12.49 and 13.7 ± 4.2μgkg−1 respectively. Occurrence of these high levels of drug residues demand mass awareness programs associated with the implementation of legislation and its enforcement designed to protect the consumer.
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References
Irum, A., Saeed, H., Ali, S., et al.: Estimation of amoxicillin residues in commercial meat and milk samples. J. Microbiol. Biotechnol. Food Sci. 4, 48–50 (2014)
Jabbar, A., Rehman, S.U.: Microbiological evaluation of antibiotic residues in meat, milk and eggs. J. Microbiol. Biotechnol. Food Sci. 2, 2349–2354 (2013)
Noori, N., Karim, G., Raeesian, M., et al.: Antibiotic residues and aflatoxin M1 contamination in milk powder used in Tehran dairy factories, Iran. Iran. J. Vet. Med. 7, 221–226 (2013)
Sats, A., Mootse, H., Lepasalu, L., et al.: Use of Delvotest T for quantitative estimation of beta lactam antibiotic residues in waste milk and for evaluation of thermal treatment efficiency- a methodical pilot study. Agron. Res. 12, 807–812 (2014)
Khaskheli, M., Malik, R.S., Arain, M.A., et al.: Detection of beta lactam antibiotic residues in market milk. Pak. J. Nutr. 7, 682–685 (2008)
Anderson, A.D., Nelson, J.M., Rossiter, S., et al.: Public health consequences of use of antimicrobial agents in food animals in the United States. Microb. Drug Resist. 9, 373–379 (2003)
Allison, J.R.D.: Antibiotic residues in milk. Br. Vet. J. 141, 9–16 (1985)
Ghidini, S., Zanardi, E., Varisco, G., et al.: Residues of B-lactam antibiotics in bovine milk: confirmatory analysis by liquid chromatography tandem mass spectrometry after microbial assay screening. Food Addit. Contam. 20, 528–534 (2003)
Graham, T., Hennes, R.F..: Beta lactam test methods for use under appendix N and section 6 of the grade “A” pasteurized milk ordinance (M-a-85, revision # 13). In: USFDA (ed.), MD, USA (2010)
Fao.: Status of and prospects for smallholder milk production- a global perspective. In: Hemme, T., Otte, J. (eds.). Rome, Italy (2010)
Afzal, M.: Re-designing smallholder dairy production in Pakistan. Pak. Vet. J. 30, 187–190 (2010)
Government of Pakistan.: Agricultural census. In: statistics PBo (ed.), Agricultural census organization, Lahore (2010)
Hemme, T.: Dairy sector and chain profile. Dairy report: for a better understanding of milk production world-wide. In: International Farm Comparison Network (IFCN), Kiel, Germany, p. 62 (2012)
Government of Pakistan.: Household integrated economic survey (HIES) 2011–12. In: statistics Pbo (ed.), Islamabad (2013)
Zia, U.: Analysis of milk marketing chain-Pakistan. Ital. J. Anim. Sci. 6, 1384–1386 (2007)
Muhammad, Z.U., Akhter, S.N., Ullah, M.K.: Dairy supply chain management and critical investigations on dairy informal channel partners in Pakistan. IOSR J. Bus. Manag. 16, 81–87 (2014)
Tariq, M., Mustafa, M.I., Iqbal, A., et al.: Milk marketing and value chain contraints. Pak. J. Agric. Sci. 45, 195–200 (2008)
Fao.: Dairy development in Pakistan. In: Zia U, Mahmood T, Ali MR (eds). Rome, Italy (2011)
Mangsi, A.S., Khaskheli, M., Soomro, A.H., et al.: Detection of antimicrobial drug residues in milk marketed at different areas of Sindh province. IOSR J. Agric. Vet. Sci. 7, 65–69 (2014)
Freitas, A., Barbosa, J., Ramos, F.: Development and validation of a multi-residue and multiclass ultra-high-pressure liquid chromatography-tandem mass spectrometry screening of antibiotics in milk. Int. Dairy J. 33, 38–43 (2013)
Aning, K.G., Donkor, E.S., Omore, A., et al.: Risk of exposure to marketed milk with antimicrobial drug residues in Ghana. Open Food Sci. J. 1, 1–5 (2007)
Kurwijila, L.R., Omore, A., Stall, S., et al.: Investigation of the risk of exposure to antimicrobial residues present in marketed milk in Tanzania. J. Food Prot. 69, 2487–2492 (2006)
Kang’ethe, E.K., Aboge, G.O., Arimi, S.M., et al.: Investigation of the risk of consuming marketed milk with antimicrobial residues in Kenya. Food Control. 16, 349–355 (2005)
Durrani, A., Mehmood, N., Shakoori, A.: Comparison of three diagnostic methods for Theileria annulata in Sahiwal and Friesian cattle in Pakistan. Pak. J. Zool. 42, 467–471 (2010)
Velusamy, R., Rani, N., Ponnudurai, G., et al.: Influence of season, age and breed on prevalence of haemoprotozoan diseases in cattle of Tamil Nadu, India. Vet. World. 7, 574–578 (2014)
Alim, M.A., Das, S., Roy, K., et al.: Prevalence of hemoprotozoan diseases in cattle population of Chittagong division, Bangladesh. Pak. Vet. J. 32, 221–224 (2012)
Acknowledgement
Authors really acknowledge the help of Biomin® Singapore for its support by providing funds to purchase kits for ELISA analysis of milk samples. Moreover, we would like to thank Mr. Sajid and Mr. Faisal in Quality Operation Lab in UVAS Lahore for their support during analysis. Moreover, Charles Sturt University’s writing support for timely submission was exceptional.
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Aslam, N. et al. (2020). Analysing Informal Milk Supply Chains Data to Identify Seasonal Occurrences of Antibiotic Residues. In: Rahman, A. (eds) Statistics for Data Science and Policy Analysis. Springer, Singapore. https://doi.org/10.1007/978-981-15-1735-8_10
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DOI: https://doi.org/10.1007/978-981-15-1735-8_10
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