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Bioactive Metabolites from Streptomyces nanhaiensis VSM-1: Polyphasic Taxonomy, Optimization, and Evaluation of Antimicrobial Metabolites by GC-MS Analysis

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Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation

Abstract

Actinomycetes strain VSM-1 isolated from the marine sediment samples of north coastal Bay of Bengal was identified as Streptomyces nanhaiensis by conventional and molecular approaches. S. nanhaiensis recorded significant broad-spectrum activity against all human and plant pathogenic microorganisms tested. An attempt has been made to evaluate the influence of medium components on the antimicrobial metabolite production of VSM-1 and to optimize an effective fermentation medium to enhance its bioactive metabolite yield. Bioactive metabolite production by VSM-1 was initially optimized using one-factor-at-a-time (OFAT) method where the yield of metabolites was recorded to generate inhibition zones of 30, 29, 28, 27, and 29 mm against test microorganisms, i.e., Shigella flexneri, Serratia marcescens, Proteus vulgaris, Pseudomonas aeruginosa, and medically important dermatophyte Candida albicans. The optimal values obtained from OFAT were selected, and the experimental model was designed using response surface methodology. Central composite design (CCD) was employed to study the influence of the variables on the production of bioactive metabolites by VSM-1 and their effect on the responses (test microorganisms). The statistical analysis showed that the variables which have a significant effect (P < 0.001) on the metabolite production at both the interactive and individual levels were measured in terms of antimicrobial activity. Maximum yield of metabolites was recorded to generate increased zones of inhibition, i.e., 34.2, 32, 31.6, 32.3, and 33.6 mm, against test microorganisms Shigella flexneri, Serratia marcescens, Proteus vulgaris, Pseudomonas aeruginosa, and Candida albicans, respectively. Unstructured kinetic models were used to fit the results obtained from experiments, and kinetic parameters were also estimated. Further, chemometric profile of the ethyl acetate extract of the strain was performed by gas chromatography and mass spectroscopy (GC-MS). Interpretation on mass spectrum of GC-MS was carried out using NIST Mass Spectral Data Base, with NIST MS search v.2.0. The results of the present study revealed the presence of various active principles with a diverse range of positive pharmacological actions, and hence this strain could be a possible source of novel bioactive compounds.

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References

  • Ajayi GO, Olagunju JA, Ademuyiwa O (2011) Gas chromatography-mass spectrometry analysis and phytochemical screening of ethanolic root extract of Plumbago zeylanica, Linn. Med Plant Res 5:1756–1761

    CAS  Google Scholar 

  • Alferova IV, Terekhova LP, Prauzer KH (1989) Selective medium with nalidixic acid for isolating antibiotic producing actinomycetes. Antibiot Khimioter 34:344–348

    CAS  PubMed  Google Scholar 

  • Almeida DG, Soares da Silva R d CF, Luna JM, Rufino RD, Santos VA, Sarubbo LA (2017) Response surface methodology for optimizing the production of biosurfactant by Candida tropicalis on industrial waste substrates. Front Microbiol 8:157

    PubMed  PubMed Central  Google Scholar 

  • Appendino G, Banfi L (2011) Molecular diversity and natural products. Mol Divers 15:291–292

    CAS  PubMed  Google Scholar 

  • Atta HM, Bahobail AS, El-Sehrawi MH (2011) Studies on isolation, classification and phylogenetic characterization of antifungal substances produced by Streptomycesalbidoflavus-143. New York Sci J 4:40–53

    Google Scholar 

  • Atta HM, Afifi MM, Elshanawany AA (2012) Production of Hygromycin-B antibiotic from Streptomyces crystallinus. AZ-A151: II. Parameters controlling of antibiotic production. Acad Arena 4:37–52

    Google Scholar 

  • Basilio A, Gonzalez I, Vicente MF (2003) Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. J Appl Microbiol 95:814–823

    CAS  PubMed  Google Scholar 

  • Boussada O, Ammar A, Saidana D (2008) Chemical composition and antimicrobial activity of volatile components from capitula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbiol Res 163:87–95

    Google Scholar 

  • Box GEP, Hunter JS (1957) Multi-factor experimental designs for exploring response surfaces. Ann Math Stat 28:1957) 195–1957) 241

    Google Scholar 

  • Bozzola JJ, Russell LD (1999) Electron microscopy principles and techniques for biologists. Jones and Bartlett Publishers, Sudbury

    Google Scholar 

  • Bredholdt H, Galatenko OA, Engelhardt K (2007) Rare actinomycete bacteria from the shallow water sediments of the Trondheim fjord, Norway: isolation, diversity, biological activity. Environ Microbiol 9:2756–2764

    CAS  PubMed  Google Scholar 

  • Chattopadhyay D, Sen SK (1997) Optimization of cultural conditions for antifungal antibiotic accumulated by Streptomyces rochei G 164. Hindustan Antibiot Bull 39:64–71

    CAS  PubMed  Google Scholar 

  • Cowan ST (1974) Cowan and Steel’s manual for the identification of medical bacteria. Cambridge University Press, Cambridge

    Google Scholar 

  • Donadio S, Sosio M, Lancini G (2002) Impact of the first Streptomyces genome sequence on the discovery and production of bioactive substances. Appl Microbiol Biotechnol 60:377–380

    CAS  PubMed  Google Scholar 

  • Ellaiah P, Srinivasulu B, Adinarayana K (2004) Optimization studies on neomycin production by a mutant strain of Streptomyces marinensis in solid state fermentation. Process Biochem 39:529–534

    CAS  Google Scholar 

  • Fatma GSB, Isil IG, Osman O (2013) Analysis of the polymer composite bipolar plate properties on the performance of PEMFC (polymer electrolyte membrane fuel cells) by RSM (response surface methodology). Energy 55:1067–1075

    Google Scholar 

  • Gordon RE (1966) Some criteria for the recognition of Nocardia madura (Vincent) Blanchord. J Gen Microbiol 45:355–364

    CAS  PubMed  Google Scholar 

  • Güray G, Altunay P, Abdurrahman T (2008) Electrochemical treatment of deproteinated whey waste water and optimization of treatment conditions with response surface methodology. J Hazard Mater 157:69–78

    Google Scholar 

  • Han WC, Lee JY, Park DH (2004) Isolation and antifungal and antioomycete activity of Streptomyces scabiei strain PK-A41, the causal agent of common scab disease. Plant Pathol J 20:115–126

    Google Scholar 

  • Hema R, Kumaravel S, Alagusundaram K (2011) GC/MS determination of bioactive components of Murraya koenigii. J Am Sci 7:80–83

    Google Scholar 

  • Hong L, Yi-min Z, **g H (2017) Optimization of vanadium (IV) extraction from stone coal leaching solution by emulsion liquid membrane using response surface methodology. Chem Eng Res Des 123:111–119

    Google Scholar 

  • Hsouna AB, Trigui M, Mansour RB (2011) Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat. Int J Food Microbiol 148:66–72

    PubMed  Google Scholar 

  • Isik K, Chun J, Hah YC, Goodfellow M (1999) Nocardia salmonicida nom. rev., a fish pathogen. Int J Syst Bacteriol 49:833–837

    PubMed  Google Scholar 

  • Jensen PR, Williams PG, Oh DC (2007) Species specific secondary metabolite production in marine actinomycetes of the genus Salinispora. Appl Environ Microbiol 73:1146–1152

    CAS  PubMed  Google Scholar 

  • Jones K (1949) Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristics. J Bacteriol 57:141–145

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ka HW, George Q, Kong M (2017) Optimisation of Pueraria isoflavonoids by response surface methodology using ultrasonic-assisted extraction. Food Chem 231:231–237

    Google Scholar 

  • Khare CP (2004) Indian herbal remedies: rational western therapy, ayurvedic and other traditional usage, botany. Springer, Berlin

    Google Scholar 

  • Koehn FE, Carter GT (2005) The evolving role of natural products in drug discovery. Nat Rev Drug Discov 4:206–220

    CAS  PubMed  Google Scholar 

  • Leal MC, Puga J, Serodio J (2012) Trends in the discovery of new marine natural products from invertebrates over the last two decades—where and what are we bioprospecting? PLoS One 7:1–15

    Google Scholar 

  • Mahmood A, Ahmed R, Kosar S (2009) Phytochemical screening and biological activities of the oil components of Prunus domestica Linn. J Saudi Chem Soc 13:273–277

    CAS  Google Scholar 

  • Majumdar MK, Majumdar SK (1965) Effect of minerals on neomycin production by Streptomyces fradiae. Appl Microbiol 13:190–193

    CAS  PubMed  PubMed Central  Google Scholar 

  • Mammen D, Daniel M, Sane RT (2010) Seasonal and geographical variations in chemical constituents of Leptadenia reticulata. Int J Pharm Sci Rev Res 4:111–116

    Google Scholar 

  • Marcos AB, Ricardo ES, Eliane PO (2008) Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta 76:965–977

    Google Scholar 

  • Mihailovi V, Vukovic N, Niciforovic N (2011) Studies on the antimicrobial activity and chemical composition of the essential oils and alcoholic extracts of Gentiana asclepiadea L. J Med Plant Res 5:1164–1174

    Google Scholar 

  • Mishra PM, Sree A (2007) Antibacterial activity and GC-MS analysis of the extract of leaves of Finlaysonia obovata (a Mangrove Plant). Asian J Plant Sci 6:168–172

    CAS  Google Scholar 

  • Mustafa O (2011) Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces spp. KGG32. Int J Agric Biol 3:317–324

    Google Scholar 

  • Myers RH, Montgomery DC, Anderson-Cook CM (2009) Response surface methodology: process and product optimization using designed experiments. Wiley, Hoboken, NJ

    Google Scholar 

  • Narayana KJP, Vijayalakshmi M (2008) Optimization of antimicrobial metabolites production by Streptomyces albidoflavus. Res J Pharmacol 2:4–7

    Google Scholar 

  • Newman DJ, Cragg GM (2007) Natural products as sources of new drugs over the last 25 years. J Nat Prod 70:461–477

    CAS  PubMed  Google Scholar 

  • Nilsson WB, Strom MS (2002) Detection and identification of bacterial pathogens of fish in kidney tissue using terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes. Dis Aquat Org 48:175–185

    CAS  PubMed  Google Scholar 

  • Parthasarathi S, Kim CJ, Kim PK, Sathya S, Manikandan M, Manikandan T, Balakrishnan K (2010) Taxonomic characterization and UV/VIS analysis of antagonistic marine actinomycete isolated from South West Coast of South Korea. Int J Med Res 1:99–105

    Google Scholar 

  • Payam R, Shohreh M, Saeid V (2017) Modeling and optimization of gelatin-chitosan micro-carriers preparation for soft tissue engineering: using response surface methodology. Mater Sci Eng C 75:545–553

    Google Scholar 

  • Pridham TG, Gottlieb D (1948) The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 56:107–114

    CAS  PubMed  PubMed Central  Google Scholar 

  • Pridham TG, Hesseltine CW, Benedict RG (1958) A guide for the classification of Streptomyces according to selected groups. Appl Microbiol 6:52–79

    CAS  PubMed  PubMed Central  Google Scholar 

  • Rama Krishna VSG, Ushakiranmayi M, Vijayalakshmi M, Sudhakar P (2016) Model-based kinetic parameters estimation in batch Pullulan fermentation using Jaggery as substrate. J Chem Pharm Res 8:217–224

    Google Scholar 

  • Ramesh S, Mathivanan N (2009) Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotechnol 25:2103–2111

    CAS  Google Scholar 

  • Rangel-Sánchez G, Castro-Mercado E, García-Pineda E (2014) Avocado roots treated with salicylic acid produce phenol-2,4-bis (1,1-dimethylethyl), a compound with antifungal activity. J Plant Physiol 17:189–198

    Google Scholar 

  • Roy RRN, Laskar S, Sen SK (2006) Dibutyl phthalate, the bioactive compound produced by Streptomyces albidoflavus 321.2. Microbiol Res 161:121–126

    CAS  PubMed  Google Scholar 

  • Salini TS, Divakaran D, Shabanamol S (2014) Antimicrobial and Immuno modulatory potential of endophytic fungi Fusarium solani isolated from Withania somnifera. World J Pharm Res 3:879–890

    Google Scholar 

  • Sathish S, Vivekanandan S (2016) Parametric optimization for floating drum anaerobic bio-digester using response surface methodology and artificial neural network. Alexandria Eng J 55:3297–3307

    Google Scholar 

  • Saurav K, Kannabiran K (2010) Diversity and optimization of process parameters for the growth of Streptomyces VITSVK 9 spp. isolated from Bay of Bengal, India. J Nat Environ Sci 1:56–65

    Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Google Scholar 

  • Singh LS, Majumdar S, Bora TC (2009) Optimization of process parameters for growth and bioactive metabolite production by a salt tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D. J Mycol Med 19:223–225

    Google Scholar 

  • Solanki R, Khanna M, Cal R (2008) Bioactive compounds from marine actinomycetes. Curr Sci 48:410–431

    CAS  Google Scholar 

  • Sun W, Dai S, Jiang S (2010) Culture dependent and culture independent diversity of actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea. Antonie Van Leeuwenhoek 98:65–75

    CAS  PubMed  Google Scholar 

  • Thakur D, Bora TC, Bordoloi GN (2009) Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces spp. 201. J Mycol Med 19:161–167

    Google Scholar 

  • Tresner HD, Hayes JA, Backus EJ (1968) Differential tolerance of Streptomyces to sodium chloride as a taxonomic aid. Appl Microbiol 16:1134–1136

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ushakiranmayi M, Vijayalakshmi M, Sudhakar P, Rama Krishna Ganduri VS, Satish Babu R (2017) Optimization of culture conditions by response surface methodology and unstructured kinetic modeling for L-Asparaginase production by Pseudonocardia endophytica VUK-10. J Appl Pharm Sci 7:42–50

    Google Scholar 

  • Waksman SA (1961) The actinomycetes, classification, identification and descriptions of genera and species. The Williams and Wilkins Co., Baltimore, MD

    Google Scholar 

  • Wang G, Deng Y, Xu X (2016) Optimization of air jet im**ement drying of okara using response surface methodology. Food Control 59:743–749

    CAS  Google Scholar 

  • Wang Y, Qin X, Huang S, Feng J (2017) Structural-borne acoustics analysis and multi-objective optimization by using panel acoustic participation and response surface methodology. Appl Acoust 116:139–151

    Google Scholar 

  • Williams ST (1989) In: Williams ST (ed) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, MD

    Google Scholar 

  • **ong ZQ, Wang JF, Hao YY, Wang Y (2013) Recent advances in the discovery and development of marine microbial natural products. Mar Drugs 11:700–717

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ying Z, Chengyou C, Mei P (2014) Diversity of nitrogen-fixing, ammonia-oxidizing, and denitrifying bacteria in biological soil crusts of a revegetation area in Horqin Sandy Land, Northeast China. Ecol Eng 71:71–79

    Google Scholar 

  • Yu H, Zhao X, Yue P (2009) Chemical communication in mammal population: urinary olfactory chemosignals in lactating female root voles (Microtus oeconomus pallas). Polish J Ecol 58:153–165

    Google Scholar 

  • Zafari A, Kianmehr MH, Abdolahzadeh R (2013) Modeling the effect of extrusion parameters on density of biomass pellet using artificial neural network. Int J Recycl Org Waste Agric 2(1):9

    Google Scholar 

  • Zhang B, Han X, Pengju G, ShuqiFang JB, Zhang B, Han X, Gu P, Fang S, Bai J (2017) Response surface methodology approach for optimization of ciprofloxacin adsorption using activated carbon derived from the residue of desilicated rice husk. J Mol Liq 238:316–325

    CAS  Google Scholar 

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Acknowledgments

The first author (UKM) is grateful to the University Grants Commission (UGC), New Delhi, Government of India, for providing financial assistance in the form of fellowship to carry out this work.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India

    Ushakiranmayi Managamuri & Muvva Vijayalakshmi

  2. Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India

    Sudhakar Poda & V. S. Rama Krishna Ganduri

  3. Department of Biotechnology, K L University, Guntur, Andhra Pradesh, India

    V. S. Rama Krishna Ganduri

  4. Department of Biotechnology, National Institute of Technology, Warangal, Telangana, India

    R. Satish Babu

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  1. Ushakiranmayi Managamuri
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Editors and Affiliations

  1. Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India

    Shaik Mahammad Khasim

  2. College of Life and Environmental Sciences, Minzu University of China, Bei**g, China

    Chunlin Long

  3. Department of Plant Science, Mahidol University, Thailand, Bangkok, Thailand

    Kanchit Thammasiri

  4. Department of Plant and Environmental Sciences, University of Copenhagen, Taastrup, Denmark

    Henrik Lutken

1 Appendix

Table 38.6 Central composite factor experimental design along with experimental and predicted values for the production of bioactive metabolite (Zone of inhibition against the responses) by Streptomyces nanhaiensis VSM-1
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Managamuri, U., Vijayalakshmi, M., Poda, S., Rama Krishna Ganduri, V.S., Satish Babu, R. (2020). Bioactive Metabolites from Streptomyces nanhaiensis VSM-1: Polyphasic Taxonomy, Optimization, and Evaluation of Antimicrobial Metabolites by GC-MS Analysis. In: Khasim, S.M., Long, C., Thammasiri, K., Lutken, H. (eds) Medicinal Plants: Biodiversity, Sustainable Utilization and Conservation. Springer, Singapore. https://doi.org/10.1007/978-981-15-1636-8_38

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