Abstract
Microfluidic paper-based analytical devices are an attractive tool for point of care diagnostics as they facilitate fast detection without the need for any sophisticated instrumentation and skilled professional. These devices are disposable, portable, and affordable; hence, they are utilized in almost all the diagnostic domains for carrying out the detection. There are various aspects associated with the paper-based devices, namely working principle, reaction mechanism, fabrication schemes (2D/3D), detection sensitivity, and readout mechanism. Over the period, continuous progress is envisioned in all these domains to enhance the sensitivity of the detection and several variants, namely miniaturization, the inclusion of nanoparticles, multi-functionalization, etc. are also explored to make the detection more efficient. This chapter provides a state of the art review of the various aspects of paper-based microfluidic devices, including their fabrication scheme, sensing methodology, and their several applications in DNA detection domain. Also, advantages, disadvantages, and future aspects of these devices are also discussed.
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References
Bhatt G, Kant R, Mishra K et al (2017) Impact of surface roughness on dielectrophoretically assisted concentration of microorganisms over PCB based platforms. Biomed Microdevices 19:28. https://doi.org/10.1007/s10544-017-0172-5
Bhatt G, Mishra K, Ramanathan G, Bhattacharya S (2019) Dielectrophoresis assisted impedance spectroscopy for detection of gold-conjugated amplified DNA samples. Sensors Actuators, B Chem 288:442–453. https://doi.org/10.1016/j.snb.2019.02.081
Cai L, Xu C, Lin SH et al (2014) A simple paper-based sensor fabricated by selective wet etching of silanized filter paper using a paper mask. Biomicrofluidics. https://doi.org/10.1063/1.4898096
Carrilho E, Martinez AW, Whitesides GM (2009a) Understanding wax printing: a simple micropatterning process for paper-based microfluidics. Anal Chem 81:7091–7095. https://doi.org/10.1021/ac901071p
Carrilho E, Phillips ST, Vella SJ et al (2009b) Paper microzone plates. Anal Chem 81:5990–5998. https://doi.org/10.1021/ac900847g
Cassano CL, Fan ZH (2013) Laminated paper-based analytical devices (LPAD): fabrication, characterization, and assays. Microfluid Nanofluidics 15:173–181. https://doi.org/10.1007/s10404-013-1140-x
Cho JH, Paek SH (2001) Semiquantitative, bar code version of immunochromatographic assay system for human serum albumin as model analyte. Biotechnol Bioeng 75:725–732. https://doi.org/10.1002/bit.10094
Cunningham JC, Brenes NJ, Crooks RM (2014) Paper electrochemical device for detection of DNA and thrombin by target-induced conformational switching. Anal Chem 86:6166–6170. https://doi.org/10.1021/ac501438y
Davaji B, Lee CH (2014) A paper-based calorimetric microfluidics platform for bio-chemical sensing. Biosens Bioelectron 59:120–126. https://doi.org/10.1016/j.bios.2014.03.022
Foster LS, Gruntfest IJ (2009) Demonstration experiments using universal indicators. J Chem Educ. https://doi.org/10.1021/ed014p274
Gong MM, Nosrati R, San Gabriel MC et al (2015) Direct DNA analysis with paper-based ion concentration polarization. J Am Chem Soc 137:13913–13919. https://doi.org/10.1021/jacs.5b08523
Han KN, Li CA, Seong GH (2013) Microfluidic chips for immunoassays. Annu Rev Anal Chem 6:119–141. https://doi.org/10.1146/annurev-anchem-062012-092616
Hu J, Wang SQ, Wang L et al (2014) Advances in paper-based point-of-care diagnostics. Biosens Bioelectron 54:585–597. https://doi.org/10.1016/j.bios.2013.10.075
Kant R, Bhatt G, Sundriyal P, Bhattacharya S (2017) Relevance of adhesion in fabrication of microarrays in clinical diagnostics. In: Mittal KL, Etzler FM (eds) Adhesion in pharmaceutical, biomedical and dental fields. Scrivener Publishing LLC, USA, pp 257–298
Kumar A, Singh P, Awasthi M, Bhattacharya S (2019) α-Fe2O3 loaded rGO nanosheets based fast response/recovery CO gas sensor at room temperature. Appl Surf Sci 465:56–66. https://doi.org/10.1016/j.apsusc.2018.09.123
Kumar S, Bhushan P, Krishna V, Bhattacharya S (2018) Tapered lateral flow immunoassay based point-of-care diagnostic device for ultrasensitive colorimetric detection of dengue NS1. Biomicrofluidics 10(1063/1):5035113
Lewis GG, Ditucci MJ, Baker MS, Phillips ST (2012) High throughput method for prototy** three-dimensional, paper-based microfluidic devices. Lab Chip 12:2630–2633. https://doi.org/10.1039/c2lc40331e
Li J, Lee EC (2015) Carbon nanotube/polymer composite electrodes for flexible, attachable electrochemical DNA sensors. Biosens Bioelectron 71:414–419. https://doi.org/10.1016/j.bios.2015.04.045
Li X, Tian J, Garnier G, Shen W (2010) Fabrication of paper-based microfluidic sensors by printing. Colloids Surf B Biointerfaces 76:564–570. https://doi.org/10.1016/j.colsurfb.2009.12.023
Liu H, **ang Y, Lu Y, Crooks RM (2012) Aptamer-based origami paper analytical device for electrochemical detection of adenosine. Angew Chem Int Edit 51:6925–6928. https://doi.org/10.1002/anie.201202929
Lu J, Ge S, Ge L et al (2012) Electrochimica acta electrochemical DNA sensor based on three-dimensional folding paper device for specific and sensitive point-of-care testing. Electrochim Acta 80:334–341. https://doi.org/10.1016/j.electacta.2012.07.024
Martinez AW, Phillips ST, Whitesides GM (2008) Three-dimensional microfluidic devices fabricated in layered paper and tape. Proc Natl Acad Sci 105:19606–19611. https://doi.org/10.1073/pnas.0810903105
Martinez AW, Phillips ST, Whitesides GM, Carrilho E (2010) Diagnostics for the develo** world: microfluidic paper-based analytical devices. Anal Chem 82:3–10. https://doi.org/10.1021/ac9013989
Mullis KB, Erlich HA, Arnheim N et al (1987) Process for amplifying, detecting, and/or cloning nucleic acid
Nakano M, Suehiro J, Konishi K et al (2011) Development of rapid oral bacteria detection apparatus based on dielectrophoretic impedance measurement method. IET Nanobiotechnol 5:25–31. https://doi.org/10.1049/iet-nbt.2010.0011
Nie J, Liang Y, Zhang Y et al (2013) One-step patterning of hollow microstructures in paper by laser cutting to create microfluidic analytical devices. Analyst 138:671–676. https://doi.org/10.1039/c2an36219h
Schilling KM, Jauregui D, Martinez AW (2013) Paper and toner three-dimensional fluidic devices: programming fluid flow to improve point-of-care diagnostics. Lab Chip 13:628–631. https://doi.org/10.1039/c2lc40984d
Scida K, Li B, Ellington AD, Crooks RM (2013) DNA detection using origami paper analytical devices. Anal Chem 85:9713–9720. https://doi.org/10.1021/ac402118a
Wei X, Tian T, Jia S et al (2015) Target-responsive DNA hydrogel mediated stop-flow microfluidic paper-based analytic device for rapid, portable and visual detection of multiple targets. Anal Chem 87:4275–4282. https://doi.org/10.1021/acs.analchem.5b00532
Wong RC, Tse HY (2008) Quantitative, false positive, and false negative issues for lateral flow immunoassays as exemplified by onsite drug screens. In: Lateral Flow Immunoassay. Humana Press, pp 1–19
**a Y, Si J, Li Z (2016) Fabrication techniques for microfluidic paper-based analytical devices and their applications for biological testing: a review. Biosens Bioelectron 77:774–789. https://doi.org/10.1016/j.bios.2015.10.032
Yafouz B, di Kadri NA, Ibrahim F (2013) Microarray dot electrodes utilizing dielectrophoresis for cell characterization. Sensors (Basel) 13:9029–9046. https://doi.org/10.3390/s130709029
Yan C, Yu S, Jiang Y et al (2015) Fabrication of paper-based microfluidic devices by plasma treatment and its application in glucose determination. Acta Chim Sin 72:1099. https://doi.org/10.6023/a14060496
Yu J, Ge L, Huang J et al (2011) Microfluidic paper-based chemiluminescence biosensor for simultaneous determination of glucose and uric acid. Lab Chip 11:1286–1291. https://doi.org/10.1039/c0lc00524j
Zhang Y, Zhou C, Nie J et al (2014) Equipment-free quantitative measurement for microfluidic paper-based analytical devices fabricated using the principles of movable-type printing. Anal Chem 86:2005–2012. https://doi.org/10.1021/ac403026c
Zhao X, Tapec-Dytioco R, Tan W (2003) Ultrasensitive DNA detection using highly fluorescent bioconjugated nanoparticles. J Am Chem Soc 125:11474–11475. https://doi.org/10.1021/ja0358854
Zhu H, Isikman SO, Mudanyali O et al (2013) Optical imaging techniques for point-of-care diagnostics. Lab Chip 13:51–67
Zou L, Li Y, Cao S, Ye B (2013) Gold nanoparticles/polyaniline Langmuir-Blodgett Film modified glassy carbon electrode as voltammetric sensor for detection of epinephrine and uric acid. Talanta 117:333–337. https://doi.org/10.1016/j.talanta.2013.09.035
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Bhatt, G., Bhattacharya, S. (2019). Paper-Based Microfluidic Devices for the Detection of DNA. In: Bhattacharya, S., Kumar, S., Agarwal, A. (eds) Paper Microfluidics. Advanced Functional Materials and Sensors. Springer, Singapore. https://doi.org/10.1007/978-981-15-0489-1_7
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