Cervical Squamous Intraepithelial Lesions: A Pathologist’s Perspective

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Preventive Oncology for the Gynecologist
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Abstract

Human papillomavirus (HPV) infection is associated with cervical squamous carcinoma and adenocarcinoma and their precursors. This chapter underscores the role of pathologists in morphologic evaluation and ancillary testing of cervical tissue, which is paramount toward successful implementation of cervical cancer screening protocols. Cytohistologic criteria for the precursor lesions (low-grade squamous intraepithelial lesion, high-grade squamous intraepithelial lesion, and SMILE), along with various methods for HPV testing, are herein discussed.

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References

  1. Reagan JW, Hicks DJ, Scott RB. A study of in-situ and squamous cell cancer of the uterine cervix. Cancer. 1953;6(6):1200–14.

    Article  CAS  Google Scholar 

  2. Reagan JW, Seidemann IL, Saracusa Y. The cellular morphology of carcinoma in situ and dysplasia or atypical hyperplasia of the uterine cervix. Cancer. 1953;6(2):224–34.

    Article  CAS  Google Scholar 

  3. Richart RM. A theory of cervical carcinogenesis. Obstet Gynecol Surv. 1969;24(7 Pt 2):874–9.

    Article  CAS  Google Scholar 

  4. zur Hausen H. Papillomaviruses in the causation of human cancers—a brief historical account. Virology. 2009;384(2):260–5.

    Article  CAS  Google Scholar 

  5. National Cancer Institute Workshop. The 1988 Bethesda system for reporting cervical/vaginal cytologic diagnoses. JAMA. 1989;262:931–4.

    Article  Google Scholar 

  6. Darragh TM, Colgan TJ, Cox JT, Heller DS, Henry MR, Luff RD, et al. The Lower Anogenital Squamous Terminology Standardization Project for HPV-associated lesions: background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology. J Low Genit Tract Dis. 2012;16:205–42.

    Article  Google Scholar 

  7. Kurman RJ, Ellenson H, Ronnett BM. Blaustein’s pathology of the female genital tract. 6th ed. New York: Springer; 2011.

    Book  Google Scholar 

  8. Singh C, Manivel JC, Truskinovsky AM, Savik K, Amirouche S, Holler J, et al. Variability of pathologists’ utilization of p16 and ki-67 immunostaining in the diagnosis of cervical biopsies in routine pathology practice and its impact on the frequencies of cervical intraepithelial neoplasia diagnoses and cytohistologic correlations. Arch Pathol Lab Med. 2014;138(1):76–87.

    Article  Google Scholar 

  9. Lee C, Laimins LA, editors. The differentiation-dependent life cycle of human papillomaviruses in keratinocytes. Boston: Springer; 2007.

    Google Scholar 

  10. Graham SV. Keratinocyte differentiation-dependent human papillomavirus gene regulation. Viruses. 2017;9(9). https://doi.org/10.3390/v9090245.

    Article  Google Scholar 

  11. Steinback A, Riemer AB. Immune evasion mechanisms of human papillomavirus: an update. Int J Cancer. 2018;142(2):224–29.

    Google Scholar 

  12. Graham SV. The human papillomavirus replication cycle, and its links to cancer progression: a comprehensive review. Clin Sci (Lond). 2017;131(17):2201–21.

    Article  CAS  Google Scholar 

  13. Doorbar J, Egawa N, Griffin H, Kranjec C, Murakami I. Human papillomavirus molecular biology and disease association. Rev Med Virol. 2015;25(Suppl 1):2–23.

    Article  CAS  Google Scholar 

  14. Mills AM, Dirks DC, Poulter MD, Mills SE, Stoler MH. HR-HPV E6/E7 mRNA in situ hybridization: validation against PCR, DNA in situ hybridization, and p16 immunohistochemistry in 102 samples of cervical, vulvar, anal, and head and neck neoplasia. Am J Surg Pathol. 2017;41(5):607–15.

    Article  Google Scholar 

  15. Thomas JT, Oh ST, Terhune SS, Laimins LA. Cellular changes induced by low-risk human papillomavirus type 11 in keratinocytes that stably maintain viral episomes. J Virol. 2001;75(16):7564–71.

    Article  CAS  Google Scholar 

  16. Bishop JA, Ma XJ, Wang H, Luo Y, Illei PB, Begum S, et al. Detection of transcriptionally active high-risk HPV in patients with head and neck squamous cell carcinoma as visualized by a novel E6/E7 mRNA in situ hybridization method. Am J Surg Pathol. 2012;36(12):1874–82.

    Article  Google Scholar 

  17. Guo M, Gong Y, Deavers M, Silva EG, Jan YJ, Cogdell DE, et al. Evaluation of a commercialized in situ hybridization assay for detecting human papillomavirus DNA in tissue specimens from patients with cervical intraepithelial neoplasia and cervical carcinoma. J Clin Microbiol. 2008;46(1):274–80.

    Article  CAS  Google Scholar 

  18. Nishino HT, Tambouret RH, Wilbur DC. Testing for human papillomavirus in cervical cancer screening: a review of indications and methodology. Cancer Cytopathol. 2011;119(4):219–27.

    Article  Google Scholar 

  19. Abreu AL, Souza RP, Gimenes F, Consolaro ME. A review of methods for detect human papillomavirus infection. Virol J. 2012;9:262.

    Article  CAS  Google Scholar 

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Correspondence to Grace N. Kim .

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Singh, C., Kim, G.N. (2019). Cervical Squamous Intraepithelial Lesions: A Pathologist’s Perspective. In: Mehta, S., Singla, A. (eds) Preventive Oncology for the Gynecologist. Springer, Singapore. https://doi.org/10.1007/978-981-13-3438-2_6

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  • DOI: https://doi.org/10.1007/978-981-13-3438-2_6

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-13-3437-5

  • Online ISBN: 978-981-13-3438-2

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