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Role of Molecular Marker in the Genetic Improvement of the Medicinal and Aromatic Plants

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Biotechnological Approaches for Medicinal and Aromatic Plants

Abstract

Several molecular markers have been developed for breeding major crops owing to their significance, ease, and suitability. Out of these DNA markers are frequently used ones; therefore, in this chapter, we describe the DNA markers to map major genes with regard to their principle, applicability, and methods. The two major classes of DNA markers are based on (i) DNA hybridization, e.g., restriction fragment polymorphism, DNA chips, etc.,. and (ii) polymerase chain reaction (PCR), e.g., SSR, RAPD, AFLP, and SNP. Develo** trait-linked markers involves the segregation of populations demonstrating target traits followed by reliable phenoty** methods. With the help of these techniques, trait-linked markers may be used in two situations: (i) in the absence of any biological information and (ii) with available information about the trait.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

EST:

Expressed sequence tag

GBS:

Genoty** by sequencing

ISSR:

Inter-simple sequence repeats

PCR:

Polymerase chain reaction

QTL:

Quantitative trait loci

RAPD:

Random amplified polymorphic DNA

RFLP:

Restriction fragment length polymorphism

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeats

STR:

Short tandem repeat

STSs:

Sequence-tagged sites

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Conflict of Interest

It is declared that the authors have no competing interests.

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Authors and Affiliations

  1. Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India

    Anubha Sharma

  2. Department of Biotechnology, School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Gaya, Bihar, India

    Nitish Kumar

  3. Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur, India

    Iti Gontia Mishra

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  1. Anubha Sharma
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  2. Nitish Kumar
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  3. Iti Gontia Mishra
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Correspondence to Anubha Sharma .

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Editors and Affiliations

  1. Department of Biotechnology, Central University of South Bihar, Panchanpur, Gaya, Bihar, India

    Nitish Kumar

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Sharma, A., Kumar, N., Mishra, I.G. (2018). Role of Molecular Marker in the Genetic Improvement of the Medicinal and Aromatic Plants. In: Kumar, N. (eds) Biotechnological Approaches for Medicinal and Aromatic Plants. Springer, Singapore. https://doi.org/10.1007/978-981-13-0535-1_25

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