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Geobacillus and Anoxybacillus spp. from Terrestrial Geothermal Springs Worldwide: Diversity and Biotechnological Applications

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Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications

Part of the book series: Microorganisms for Sustainability ((MICRO,volume 8))

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Abstract

A large number of thermophilic representatives of the Geobacillus and Anoxybacillus genera have been isolated from geographically distant and physicochemically different environments, including high-, moderate-, and low-temperature habitats. However, terrestrial hot springs are the main habitats for Geobacillus and Anoxybacillus species. The members of these genera possess a variety of thermo-adaptive features that enable them to thrive at elevated temperatures. Due to their ability to withstand harsh environmental conditions, geobacilli and anoxybacilli are a valuable source for provision of thermostable enzymes, such as amylases, lipases, proteases, etc., and other components. Thermostable enzymes obtained from thermophilic bacilli have found a plethora of commercial applications due to their sturdiness and toughness in withstanding the heat generated in various biotechnological and industrial processes. This chapter contains a review of studies of geobacilli and anoxybacilli from terrestrial geothermal springs worldwide with special emphasis on their distribution and diversity, ecological significance, adaptive mechanisms, enzymes, and biotechnological potential.

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References

  • Acer Ö, Bekler FM, Pirinççioğlu H, Güven RG, Güven K (2016) Purification and Characterization of Thermostable and Detergent-Stable α-Amylase from Anoxybacillus sp. AH1. Food Technol Biotechnol 54(1):70–77

    PubMed  PubMed Central  CAS  Google Scholar 

  • Acer Ö, Pırınççiğlu H, Bekler Fatma M, Gül-Güven R (2015) Anoxybacillus sp. AH1, an α-amylase-producing thermophilic bacterium isolated from Dargeçit hot spring. Biologia Sect Cell Mol Biol 70(7):853–862

    CAS  Google Scholar 

  • Agüloğlu Fincan S, Enez B, Özdemir S, Matpan Bekler F (2014) Purification and characterization of thermostable α-amylase from thermophilic Anoxybacillus flavithermus. Carbohydr Polym 102(Supplement C):144–150

    Article  PubMed  CAS  Google Scholar 

  • Ahmad S, Scopes RK, Rees GN, Patel BKC (2000) Saccharococcus caldoxylosilyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 50:517–523

    Article  PubMed  CAS  Google Scholar 

  • Al-Batayneh KM, Jacob JH, Hussein EI (2011) Isolation and molecular identification of new thermophilic bacterial strains of Geobacillus pallidus and Anoxibacillus flavithermus. Int J Integr Biol 11(1):39–43

    CAS  Google Scholar 

  • Al-Jailawi MH, Mahdi MS, Fadhil AMA (2016) Investigation the Consumption of Aromatic Hydrocarbons by Anoxybacillus rupeinsis Ir3 (JQ912241) using FTIR and HPLC Analyses. Br Biotechnol J 10(2):1–12 Article no. BBJ.18203

    Article  Google Scholar 

  • Al-Qodah Z (2006) Production and characterization of thermostable alpha-amylase by thermophilic Geobacillus stearothermophilus. Biotechnol J 1(7-8):850–857

    Article  PubMed  CAS  Google Scholar 

  • Antranikian G (2007) Industrial relevance of thermophiles and their enzymes. In: Thermophiles. CRC Press, pp 113–160

    Chapter  Google Scholar 

  • Arena A, Maugeri TL, Pavone B, Iannello D, Gugliandolo C, Bisignano G (2006) Antiviral and immunoregulatory effect of a novel exopolysaccharide from a marine thermotolerant Bacillus licheniformis. Int Immunopharmacol 6(1):8–13

    Article  PubMed  CAS  Google Scholar 

  • Arpigny JL, Jaeger KE (1999) Bacterial lipolytic enzymes: classification and properties. Biochem J 343(Pt 1):177–183

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Arya M, Joshi GK, Gupta AK, Kumar A, Raturi A (2015) Isolation and characterization of thermophilic bacterial strains from Soldhar (Tapovan) hot spring in Central Himalayan Region, India. Ann Microbiol 65(3):1457–1464. https://doi.org/10.1007/s13213-014-0984-y

    Article  CAS  Google Scholar 

  • Ash C, Farrow JAE, Wallbanks S, Collins MD (1991) Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small subunit ribosomal RNA sequences. Lett Appl Microbiol 13:202–206

    Article  CAS  Google Scholar 

  • Atanassova M, Derekova A, Mandeva R, Sjoholm C, Kambourova M (2008) Anoxybacillus bogrovensis sp. nov., a novel thermophilic bacterium isolated from a hot spring in Dolni Bogrov, Bulgaria. Int J Syst Evol Microbiol 58(Pt 10):2359–2362

    Article  PubMed  CAS  Google Scholar 

  • Ay F, Karaoglu H, Inan K, Canakci S, Belduz AO (2011) Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1. Protein Expr Purif 80(1):74–79

    Article  PubMed  CAS  Google Scholar 

  • Balan A, Ibrahim D, Abdul Rahim R, Ahmad Rashid FA (2012) Purification and characterization of a thermostable lipase from Geobacillus thermodenitrificans IBRL-nra. Enzyme Res 2012:987523. https://doi.org/10.1155/2012/987523

    Article  PubMed  PubMed Central  Google Scholar 

  • Baltas N, Dincer B, Ekinci AP, Kolayli S, Adiguzel A (2016) Purification and characterization of extracellular α-amylase from a thermophilic Anoxybacillus thermarum A4 strain, Braz. arch. biol. technol. Eng Technol Tech 59:e16160346. https://doi.org/10.1590/1678-4324-2016160346

    Article  CAS  Google Scholar 

  • Banat IM, Marchant R, Rahman TJ (2004) Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. Int J Syst Evol Microbiol 54:2197–2201

    Article  PubMed  CAS  Google Scholar 

  • Belduz AO, Canakci S, Chan K-G, Kahar UM, Chan CS, Yaakop AS, Goh KM (2015) Genome sequence of Anoxybacillus ayderensis AB04(T) isolated from the Ayder hot spring in Turkey. Stand Genomic Sci 10:70. https://doi.org/10.1186/s40793-015-0065-2

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Belduz AO, Dulger S, Demirbag Z (2003) Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53(Pt 5):1315–1320

    Article  PubMed  CAS  Google Scholar 

  • Bergey DH, Whitman WB, De Vos P, Garrity GM and Jones D (2009) Bergey’s manual of systematic bacteriology. 3, New York: Springer.: https://doi.org/10.1002/9781118960608.gbm00529

  • Bertoldo C, Antranikian G (2002) Starch-hydrolyzing enzymes from thermophilic archaea and bacteria. Curr Opin Chem Biol 6(2):151–160. https://doi.org/10.1016/S1367-5931(02)00311-3

    Article  PubMed  CAS  Google Scholar 

  • Bruins ME, Janssen AE, Boom RM (2001) Thermozymes and their applications: a review of recent literature and patents. Appl Biochem Biotechnol 90(2):155–186

    Article  PubMed  CAS  Google Scholar 

  • Brumm P, Land ML, Hauser LJ, Jeffries CD, Chang Y-J, Mead DA (2015) Complete Genome Sequence of Geobacillus strain Y4.1MC1, a Novel CO-Utilizing Geobacillus thermoglucosidasius Strain Isolated from Bath Hot Spring in Yellowstone National Park. BioEnergy Res 8(3):1039–1045

    Article  CAS  Google Scholar 

  • Bryanskaya AV, Rozanov AS, Slynko NM, Shekhovtsov SV, Peltek SE (2015) Geobacillus icigianus sp. nov., a thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 65(Pt 3):864–869

    Article  PubMed  CAS  Google Scholar 

  • Caccamo D, Gugliandolo C, Stackebrandt E, Maugeri TL (2000) Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent. Int J Syst Evol Microbiol 50:2009–2012

    Article  PubMed  Google Scholar 

  • Canakci S, Inan K, Kacagan M, Belduz AO (2007) Evaluation of arabinofuranosidase and xylanase activities of Geobacillus spp. isolated from some hot springs in Turkey. J Microbiol Biotechnol 17(8):1262–1270

    PubMed  CAS  Google Scholar 

  • Carrasco-Lopez C, Godoy C, de Las Rivas B, Fernandez-Lorente G, Palomo JM, Guisan JM, Fernandez-Lafuente R, Martinez-Ripoll M and Hermoso JA (2009) Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements, J Biol Chem 7. 4365-4372

    Article  CAS  Google Scholar 

  • Chai KP, Othman NF, Teh AH, Ho KL, Chan KG, Shamsir MS, Goh KM, Ng CL (2016) Crystal structure of Anoxybacillus alpha-amylase provides insights into maltose binding of a new glycosyl hydrolase subclass. Sci Rep 6:23126. https://doi.org/10.1038/srep23126

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chai YY, Kahar UM, Md Salleh M, Md Illias R, Goh KM (2012) Isolation and characterization of pullulan-degrading Anoxybacillus species isolated from Malaysian hot springs. Environ Technol 33(10-12):1231–1128

    Article  PubMed  CAS  Google Scholar 

  • Chakravorty D, Patra S (2013) Attaining Extremophiles and Extremolytes: Methodologies and Limitations. In: Singh OV (ed) Extremophiles: sustainable resources and biotechnological implications, 1st edn. Wiley, Hoboken, pp 29–74

    Google Scholar 

  • Chang YW, Sun YJ, Wang C, Hsiao CD (2008) Crystal structures of the 70-kDa heat shock proteins in domain disjoining conformation. J Biol Chem 283(22):15502–15511

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chatziefthimiou AD, Crespo-Medina M, Wang Y, Vetriani C, Barkay T (2007) The isolation and initial characterization of mercury resistant chemolithotrophic thermophilic bacteria from mercury rich geothermal springs. Extremophiles 11(3):469–479. https://doi.org/10.1007/s00792-007-0065-2

    Article  PubMed  CAS  Google Scholar 

  • Che Ibrahim MA, Ahmad WA (2017) Growth optimization of a thermophilic strain Geobacillus caldoxylosilyticus utm6 isolated from selayang hot spring. eProc Chem 2(1):119–123

    Google Scholar 

  • Chis L, Hriscu M, Bica A, Tosa M, Nagy G, Rona G, Vertessy BG, Dan Irimie F (2013) Molecular cloning and characterization of a thermostable esterase/lipase produced by a novel Anoxybacillus flavithermus strain. J Gen Appl Microbiol 59(2):119–134

    Article  PubMed  CAS  Google Scholar 

  • Cihan AC, Cokmus C, Koc M, Ozcan B (2014) Anoxybacillus calidus sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant. Int J Syst Evol Microbiol 64(Pt 1):211–219

    Article  PubMed  Google Scholar 

  • Cihan AC, Ozcan B, Cokmus C (2011) Anoxybacillus salavatliensis sp. nov., an alpha-glucosidase producing, thermophilic bacterium isolated from Salavatli, Turkey. J Basic Microbiol 51(2):136–146

    Article  PubMed  CAS  Google Scholar 

  • Colak A, Sisik D, Saglam N, Guner S, Canakci S, Belduz AO (2005) Characterization of a thermoalkalophilic esterase from a novel thermophilic bacterium, Anoxybacillus gonensis G2. Bioresour Technol 96(5):625–631

    Article  PubMed  CAS  Google Scholar 

  • Coorevits A, Dinsdale AE, Halket G, Lebbe L, De Vos P, Van Landschoot A, Logan NA (2012) Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov. Int J Syst Evol Microbiol 62(Pt 7):1470–1485

    Article  PubMed  CAS  Google Scholar 

  • Correa-Llantén DN, Muñoz-Ibacache SA, Castro ME, Muñoz PA, Blamey JM (2013) Gold nanoparticles synthesized by Geobacillus sp. strain ID17 a thermophilic bacterium isolated from Deception Island, Antarctica. BMC Microb Cell Fact 12(75):1–6. https://doi.org/10.1186/1475-2859-12-75

    Article  CAS  Google Scholar 

  • Cortez Y, Paul S, Villena G, Gutiérrez-Correa M (2016) Isolation and characterization of cellulase producing bacterial strains from an Amazonian geothermal spring in Peru. Br Microbiol Res J 15(5):1–8

    Article  CAS  Google Scholar 

  • Dai J, Liu Y, Lei Y, Gao Y, Han F, **ao Y, Peng H (2011) A new subspecies of Anoxybacillus flavithermus ssp. yunnanensis ssp. nov. with very high ethanol tolerance. FEMS Microbiol Lett 320(1):72–78

    Article  PubMed  CAS  Google Scholar 

  • Daupan SMMV, Rivera WL (2015) Isolation and characterization of culturable thermophilic bacteria from hot springs in Benguet, Philippines. Int J Philipp Sci Technol 8(2):14–18

    Article  Google Scholar 

  • De Clerck E, Rodríguez-Díaz M, Vanhoutte T, Heyrman J, Logan NA, De Vos P (2004) Anoxybacillus contaminans sp. nov., and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. Int J Syst Evol Microbiol 54:941–946

    Article  PubMed  CAS  Google Scholar 

  • De Maayer P, Brumm PJ, Mead DA, Cowan DA (2014) Comparative analysis of the Geobacillus hemicellulose utilization locus reveals a highly variable target for improved hemicellulolysis. BMC Genomics 15(1):836. https://doi.org/10.1186/1471-2164-15-836

    Article  PubMed  PubMed Central  Google Scholar 

  • Deep K, Poddar A, Whitman WB, Das SK (2016) Draft Genome Sequence of Anoxybacillus suryakundensis Strain JS1T (DSM 27374T) Isolated from a Hot Spring in Jharkhand, India. Genome Announc 4(4):pii: e00824-16. https://doi.org/10.1128/genomeA.00824-16

    Article  Google Scholar 

  • Derekova A, Mandeva R, Kambourova M (2008) Phylogenetic diversity of thermophilic carbohydrate degrading bacilli from Bulgarian hot springs. World J Microbiol Biotechnol 24(9):1697–1702

    Article  CAS  Google Scholar 

  • Derekova A, Sjoholm C, Mandeva R, Kambourova M (2007) Anoxybacillus rupiensis sp. Nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria). Extremophiles 11(4):577–583

    Article  PubMed  Google Scholar 

  • Derekova A, Sjoholm C, Mandeva R, Michailova L, Kambourova M (2006) Biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of Geobacillus stearothermophilus 98. Extremophiles 10(4):321–326

    Article  PubMed  CAS  Google Scholar 

  • De Stefano D, Tommonaro G, Simeon V, Poli A, Nicolaus B, Carnuccio R (2007) A polysaccharide from tomato (Lycopersicon esculentum) peels affects NF-jB activation in LPS-stimulated J774 macrophages. J Nat Prod 70:1636–1639

    Article  PubMed  CAS  Google Scholar 

  • Dheeran P, Kumar S, Jaiswal YK, Adhikari DK (2010) Characterization of hyperthermostable alpha-amylase from Geobacillus sp. IIPTN. Appl Microbiol Biotechnol 86(6):1857–1866

    Article  PubMed  CAS  Google Scholar 

  • Donk PJ (1920) A Highly Resistant Thermophilic Organism. Journal of Bacteriology 5(4):373–374

    PubMed  PubMed Central  CAS  Google Scholar 

  • Dulger S, Demirbag Z, Belduz AO (2004) Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov. Int J Syst Evol Microbiol 54(Pt 5):1499–1503

    Article  PubMed  CAS  Google Scholar 

  • Eijsink VG, Matthews BW, Vriend G (2011) The role of calcium ions in the stability and instability of a thermolysin-like protease. Protein Sci 20(8):1346–1355

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ellis JT, Magnuson TS (2012) Thermostable and Alkalistable Xylanases Produced by the Thermophilic Bacterium Anoxybacillus flavithermus TWXYL3. ISRN Microbiology 12:ID 517524. https://doi.org/10.5402/2012/517524

    Article  CAS  Google Scholar 

  • England JL, Shakhnovich BE, Shakhnovich EI (2003) Natural selection of more designable folds: a mechanism for thermophilic adaptation. Proc Natl Acad Sci USA 100(15):8727–8731

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Feitkenhauer H, Muller R, Markl H (2003) Degradation of polycyclic aromatic hydrocarbons and long chain alkanes at 60–70 degrees C by Thermus and Bacillus spp [corrected]. Biodegradation 14(6):367–372

    Article  PubMed  CAS  Google Scholar 

  • Filippidou S, Jaussi M, Junier T, Wunderlin T, Jeanneret N, Palmieri F, Palmieri I, Roussel-Delif L, Vieth-Hillebrand A, Vetter A, Chain PS, Regenspurg S, Junier P (2016) Anoxybacillus geothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station. Int J Syst Evol Microbiol 66(8):2944–2951

    Article  PubMed  CAS  Google Scholar 

  • Fortina MG, Mora D, Schumann P, Parini C, Manachini PL, Stackebrandt E (2001) Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov. Int J Syst Evol Microbiol 51:2063–2071

    Article  PubMed  CAS  Google Scholar 

  • Genc B, Nadaroglu H, Adiguzel A, Baltaci O (2015) Purification and characterization of an extracellular cellulase from Anoxybacillus gonensis O9 isolated from geothermal area in Turkey. J Environ Biol 36(6):1319–1324

    PubMed  CAS  Google Scholar 

  • Goh KM, Gan HM, Chan K-G, Chan GF, Shahar S, Chong CS, Kahar UM, Chai KP (2014) Analysis of Anoxybacillus genomes from the aspects of lifestyle adaptations, prophage diversity, and carbohydrate metabolism. PLOS One 9(3):e90549

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Graham R, Ternan N, McMullan G (2005) Proteomic investigation of Geobacillus thermoleovorans T80. In: Proceedings of international symposium on extremophiles and their applications, pp 97–101

    Google Scholar 

  • Gudiukaite R, Sadauskas M, Gegeckas A, Malunavicius V, Citavicius D (2017) Construction of a novel lipolytic fusion biocatalyst GDEst-lip for industrial application. J Ind Microbiol Biotechnol 44(6):799–815

    Article  PubMed  CAS  Google Scholar 

  • Gudiukaite R, Gegeckas A, Kazlauskas CD (2014) Influence of N- and C-terminal regions on activity, expression, characteristics and structure of lipase from Geobacillus sp. 95. Extremophiles 18:131–145

    Article  PubMed  CAS  Google Scholar 

  • Guezennec J (2002) Deep-sea hydrothermal vents: A new source of innovative bacterial exopolysaccharides of biotechnological interest. J Ind Microbiol Biotechnol 29:204–208

    Article  PubMed  CAS  Google Scholar 

  • Gul-Guven R, Guven K, Poli A, Nicolaus B (2008) Anoxybacillus kamchatkensis subsp. asaccharedens subsp. nov., a thermophilic bacterium isolated from a hot spring in Batman. J Gen Appl Microbiol 54(6):327–334

    Article  PubMed  CAS  Google Scholar 

  • Gurumurthy DM, Neelagund SE (2010) Geobacillus sp. Iso 5, a novel amylase-producing thermophile from thermal springs in Konkan region of Southern India. J Earth Sci 21(1):319–322

    Article  CAS  Google Scholar 

  • Gurumurthy DM, Neelagund SE (2012) Molecular characterization of industrially viable extreme thermostable novel α-amylase of Geobacillus sp. Iso5 isolated from geothermal spring. J Pure Appl Microbiol 6(4):1–15

    Google Scholar 

  • Hawumba JF, Theron J, Brozel VS (2002) Thermophilic protease-producing Geobacillus from Buranga hot springs in Western Uganda. Curr Microbiol 45(2):144–150

    Article  PubMed  CAS  Google Scholar 

  • Heinen W, Lauwers AM, Mulders JW (1982) Bacillus flavothermus, a newly isolated facultative thermophile. Antonie Van Leeuwenhoek 48(3):265–272

    Article  PubMed  CAS  Google Scholar 

  • Higgs PG, Ran W (2008) Coevolution of codon usage and tRNA genes leads to alternative stable states of biased codon usage. Mol Biol Evol 25(11):2279–2291

    Article  PubMed  CAS  Google Scholar 

  • Hovhannisyan P, Panosyan H, Turabyan A, Trchounian A (2016) Termostable amylase producing bacilli isolated from Armenian geothermal springs. Biol J Armenia 68(Special Issue):68–73

    Google Scholar 

  • Hussein AH, Lisowska BK, Leak DJ (2015) The genus Geobacillus and their biotechnological potential. Adv Appl Microbiol 92:1–48

    Article  PubMed  Google Scholar 

  • Ibrahim ASS, El-diwany AI (2007) Isolation and identification of new cellulases producing thermophilic bacteria from an Egyptian hot spring and some properties of the crude enzyme. Aust J Basic Appl Sci 1(4):473–478

    CAS  Google Scholar 

  • Inan K, Belduz AO, Canakci S (2013) Anoxybacillus kaynarcensis sp. nov., a moderately thermophilic, xylanase producing bacterium. J Basic Microbiol 53(5):410–419

    Article  PubMed  CAS  Google Scholar 

  • Inan K, Canakci S, Belduz A (2011) Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turk J Biol 35:529–542

    CAS  Google Scholar 

  • Jorquera MA, Gabler S, Inostroza NG, Acuna JJ, Campos MA, Menezes-Blackburn D, Greiner R (2018) Screening and characterization of phytases from bacteria isolated from Chilean hydrothermal environments. Microb Ecol 75(2):387–399

    Article  PubMed  CAS  Google Scholar 

  • Jugran J, Rawat N, Joshi G (2015) Amylase production by Geobacillus sp. GJA1 isolated from a hot spring in Uttarakhand. ENVIS Himalayan Ecol 23:120–125

    Google Scholar 

  • Junge K, Eicken H, Deming JW (2004) Bacterial Activity at −2 to −20 degrees C in Arctic wintertime sea ice. Appl Environ Microbiol 70(1):550–557

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kacagan M, Canakci S, Sandalli C, Inan K, Colak DN, Belduz AO (2008) Characterization of a xylanase from a thermophilic strain of Anoxybacillus pushchinoensis A8. Biologia 63(5):599–606

    Article  CAS  Google Scholar 

  • Kahar UM, Sani MH, Chan KG, Goh KM (2016) Immobilization of alpha-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports. Molecules 21(9):1196. https://doi.org/10.3390/molecules21091196

    Article  CAS  PubMed Central  Google Scholar 

  • Kambourova M, Mandeva R, Dimova D, Poli A, Nicolaus B, Tommonaro G (2009) Production and characterization of a microbial glucan, synthesized by Geobacillus tepidamans V264 isolated from Bulgarian hot spring. Carbohydrate Polymers 77(2):338–343

    Article  CAS  Google Scholar 

  • Kevbrin VV, Zengler K, Lysenko AM, Wiegel J (2005) Anoxybacillus kamchatkensis sp. nov., a novel thermophilic facultative aerobic bacterium with a broad pH optimum from the Geyser valley, Kamchatka. Extremophiles 9(5):391–398

    Article  PubMed  CAS  Google Scholar 

  • Kikani BA, Singh SP (2012) The stability and thermodynamic parameters of a very thermostable and calcium-independent α-amylase from a newly isolated bacterium, Anoxybacillus beppuensis TSSC-1. Process Biochem 47(12):1791–1798

    Article  CAS  Google Scholar 

  • Khalil A, Sivakumar N, Qarawi S (2015) Genome Sequence of Anoxybacillus flavithermus Strain AK1, a Thermophile Isolated from a Hot Spring in Saudi Arabia. Genome Announc 3(3):e00604-15

    Article  PubMed  PubMed Central  Google Scholar 

  • Kornmann H, Duboc P, Marison I, von Stockar U (2003) Influence of nutritional factors on the nature, yield, and composition of exopolysaccharides produced by Gluconacetobacter xylinus I-2281. Appl Environ Microbiol 69(10):6091–6098

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kuisiene N, Raugalas J, Chitavichius D (2004) Geobacillus lituanicus sp. nov. Int J Syst Evol Microbiol 54:1991–1995

    Article  PubMed  CAS  Google Scholar 

  • Kuhad RC, Gupta R, Singh A (2011) Microbial Cellulases and Their Industrial Applications. Enzyme Res 2011(280696). https://doi.org/10.4061/2011/280696

    Article  CAS  Google Scholar 

  • Kumar V, Verma D, Archana A, Satyanarayana T (2013) Thermostable Bacterial Xylanases. In: Satyanarayana T, Littlechild J, Kawarabayasi Y (eds) Thermophilic microbes in environmental and industrial biotechnology: biotechnology of thermophiles. Springer, Dordrecht, pp 813–857

    Chapter  Google Scholar 

  • Ladeira SA, Cruz E, Delatorre AB, Barbosa JB, Martins MLL (2015) Cellulase production by thermophilic Bacillus sp. SMIA-2 and its detergent compatibility. Electron J Biotechnol 18(2):110–115. https://doi.org/10.1016/j.ejbt.2014.12.008

    Article  CAS  Google Scholar 

  • Lee SJ, Lee YJ, Ryu N, Park S, Jeong H, Kim BC, Lee DW, Lee HS (2012) Draft genome sequence of the thermophilic bacterium Anoxybacillus kamchatkensis G10. J Bacteriol 194(23):6684–6685

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Leow TC, Rahman RN, Basri M, Salleh AB (2004) High level expression of thermostable lipase from Geobacillus sp. strain T1. Biosci Biotechnol Biochem 68(1):96–103

    Article  PubMed  CAS  Google Scholar 

  • Lim YL, Chan KG, Ee R, Belduz AO, Canakci S, Kahar UM, Yaakop AS, Goh KM (2015) Complete genome of the potential thermozyme producer Anoxybacillus gonensis G2(T) isolated from the Gonen hot springs in Turkey. J Biotechnol 212:65–66. https://doi.org/10.1016/j.jbiotec.2015.08.007

    Article  PubMed  CAS  Google Scholar 

  • Liu Y, Li R, Wang J, Zhang X, Jia R, Gao Y, Peng H (2017) Increased enzymatic hydrolysis of sugarcane bagasse by a novel glucose- and xylose-stimulated β-glucosidase from Anoxybacillus flavithermus subsp. yunnanensis E13(T). BMC Biochem 18:art4, 1–8. https://doi.org/10.1186/s12858-017-0079-z

    Article  CAS  Google Scholar 

  • Llarch A, Logan NA, Castellvi J, Prieto MJ, Guinea J (1997) Isolation and characterization of thermophilic Bacillus spp. from geothermal environments on Deception Island, South Shetland Archipelago. Microb Ecol 34(1):58–65

    Article  PubMed  CAS  Google Scholar 

  • Lobry JR, Chessel D (2003) Internal correspondence analysis of codon and amino-acid usage in thermophilic bacteria. J Appl Genet 44(2):235–261

    PubMed  Google Scholar 

  • Lotti M, Alberghina L (2007) Lipases: molecular structure and function. In: Polaina J, MacCabe AP (eds) Industrial enzymes: structure, function and applications, vol 16. Springer, Dordrecht, pp 264–282

    Google Scholar 

  • Mahadevan GD, Neelagund SE (2014) Thermostable lipase from Geobacillus sp. Iso5: bioseparation, characterization and native structural studies. J Basic Microbiol 54(5):386–396. https://doi.org/10.1002/jobm.201200656

    Article  PubMed  CAS  Google Scholar 

  • Mahadevan GD, Neelagund SE (2010) Geobacillus sp. Iso 5, a novel amylase producing thermophile from konkan region thermal springs in southern India. J Earth Sci 21:318–322

    Google Scholar 

  • Matpan Bekler F, Acer O, Guven K (2015) Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1. Cell Mol Biol (Noisy-le-grand) 61(4):113–120

    CAS  Google Scholar 

  • Matpan Bekler F, Güven K (2014) Isolation and production of thermostable α-amylase from thermophilic Anoxybacillus sp. KP1 from Diyadin hot spring in Ağri, Turkey. Biologia 69(4):419–427

    Article  CAS  Google Scholar 

  • Mehta D, Satyanarayana T (2013a) Biochemical and molecular characterization of recombinant acidic and thermostable raw-starch hydrolysing α-amylase from an extreme thermophile Geobacillus thermoleovorans. J Mol Catal B: Enzym 85–86(0):229–238

    Article  CAS  Google Scholar 

  • Mehta D, Satyanarayana T (2013b) Diversity of hot environments and thermophilic microbes’. In: Satyanarayana T, Littlechild J, Kawarabayasi Y (eds) Thermophilic microbes in environmental and industrial biotechnology: biotechnology of thermophiles. Springer, Dordrecht, pp 3–60

    Chapter  Google Scholar 

  • Mehta A, Kumar R, Gupta R (2012) Isolation of lipase producing thermophilic bacteria: optimization of production and reaction conditions for lipase from Geobacillus sp. Acta Microbiol Immunol Hung 59(4):435–450

    Article  PubMed  CAS  Google Scholar 

  • Miñana-Galbis D, DL P´ n, JG L´n, Manresa A, OliartRos RM (2010) Reclassification of Geobacillus pallidus (Scholz et al. 1988) Banat et al. 2004 as Aeribacillus pallidus gen. nov., comb. nov. Int J Syst Evol Microbiol 60:1600–1604

    Article  PubMed  Google Scholar 

  • Mollania N, Khajeh K, Hosseinkhani S, Dabirmanesh B (2010) Purification and characterization of a thermostable phytate resistant alpha-amylase from Geobacillus sp. LH8. Int J Biol Macromol 46(1):27–36

    Article  PubMed  CAS  Google Scholar 

  • Moonmangmee S, Toyama H, Adachi O, Teerakool G, Lotong N, Matsushita K (2002) Purification and characterization of a novel polysaccharide involved in the pellicle produced by a thermotolerant Acetobacter strain. Biosci Biotechnol Biochem 66:777–783

    Article  PubMed  CAS  Google Scholar 

  • Muhd Sakaff MK, Abdul Rahman AY, Saito JA, Hou S, Alam M (2012) Complete genome sequence of the thermophilic bacterium Geobacillus thermoleovorans CCB_US3_UF5. J Bacteriol 194(5):1239

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Musto H, Naya H, Zavala A, Romero H, Alvarez-Valin F, Bernardi G (2005) The correlation between genomic G+C and optimal growth temperature of prokaryotes is robust: a reply to Marashi and Ghalanbor. Biochem Biophys Res Commun 330(2):357–360

    Article  PubMed  CAS  Google Scholar 

  • Musto H, Naya H, Zavala A, Romero H, Alvarez-Valin F, Bernardi G (2006) Genomic GC level, optimal growth temperature, and genome size in prokaryotes. Biochem Biophys Res Commun 347(1):1–3

    Article  PubMed  CAS  Google Scholar 

  • Nakamichi T, Nakashima T, Fujisaki H, Nobue T, Muramatsu T, Takahashi Y, Ishibashi Y (2010) Characteristics of Anoxybacillus sp. MU3 isolated from a hot spring and its application to the hyper thermal solubilization of sewage sludge. Environ Eng Sci 27(12):993–999

    Article  CAS  Google Scholar 

  • Namsaraev ZB, Babasanova OB, Dunaevsky YE, Akimov VN, Barkhutova DD, Gorlenko VM, Namsaraev BB (2010) Anoxybacillus mongoliensis sp. nov., a novel thermophilic proteinase producing bacterium isolated from alkaline hot spring, central Mongolia. Mikrobiologiia 79(4):516–523

    PubMed  CAS  Google Scholar 

  • Narayan VV, Hatha MA, Morgan HW, Rao D (2008) Isolation and characterization of aerobic thermophilic bacteria from the savusavu hot springs in fiji. Microbes Environ 23(4):350–352

    Article  PubMed  Google Scholar 

  • Nazina TN, Lebedeva EV, Poltaraus AB, Tourova TP, Grigoryan AA, Sokolova D, Lysenko AM, Osipov GA (2004) Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. Int J Syst Evol Microbiol 54(Pt 6):2019–2024

    Article  PubMed  CAS  Google Scholar 

  • Nazina TN, Sokolova D, Grigoryan AA, Shestakova NM, Mikhailova EM, Poltaraus AB, Tourova TP, Lysenko AM, Osipov GA, Belyaev SS (2005) Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species. Syst Appl Microbiol 28(1):43–53

    Article  PubMed  CAS  Google Scholar 

  • Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE, Lysenko AM, Petrunyaka VV, Osipov GA, Belyaev SS, Ivanov MV (2001) Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th. Int J Syst Evol Microbiol 51(Pt 2):433–446

    Article  PubMed  CAS  Google Scholar 

  • Niehaus F, Bertoldo C, Kahler M, Antranikian G (1999) Extremophiles as a source of novel enzymes for industrial application. Appl Microbiol Biotechnol 51(6):711–729

    Article  PubMed  CAS  Google Scholar 

  • Nicolaus B, Manca MC, Lama L, Esposito E, Gambacorta A (1995) Effects of growth temperature on the polar lipid pattern and fatty acid composition of seven thermophilic isolates from the Antarctic continent. Syst Appl Microbiol 18(1):32–36

    Article  CAS  Google Scholar 

  • Nichols CM, Lardiere SG, Bowman JP, Nichols PD, Gibson JAE, Guezennec J (2005) Chemical characterization of exopolysaccharides from Antarctic marine bacteria. Microb Ecol 49(4):578–589

    Article  PubMed  CAS  Google Scholar 

  • Nwodo UU, Green E, Okoh AI (2012) Bacterial exopolysaccharides: functionality and prospects. Int J Mol Sci 13(11):14002–14015

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Obeidat M, Khyami-Horani H, Al-Zoubi A, Otri I (2012) Isolation, characterization, and hydrolytic activities of Geobacillus species from Jordanian hot springs. Afr J Biotechnol 11(25):6763–6768

    CAS  Google Scholar 

  • Ogg CD, Spanevello MD, Patel BKC (2013) Exploring the ecology of thermophiles from Australia’s Great Artesian basin during the genomic era. In: Satyanarayana T, Littlechild J, Kawarabayasi Y (eds) Thermophilic microbes in environmental and industrial biotechnology: biotechnology of thermophiles. Springer, Dordrecht, pp 61–97

    Chapter  Google Scholar 

  • Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, Harel M, Remington SJ, Silman I, Schrag J et al (1992) The alpha/beta hydrolase fold. Protein Eng 5(3):197–211

    Article  PubMed  CAS  Google Scholar 

  • Olusesan AT, Azura LK, Abubakar F, Hamid NSA, Radu S, Saari N (2009) Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. World J Microbiol Biotechnol 25(11):1981–1988

    Article  CAS  Google Scholar 

  • Ortiz EM, Berretta MF, Navas LE, Benintende GB, Amadio AF, Zandomeni RO (2015) Draft genome sequence of Geobacillus sp. Isolate T6, a thermophilic bacterium collected from a thermal spring in Argentina. Genome Announc 3(4):e00743-15

    Article  PubMed  PubMed Central  Google Scholar 

  • Ozdemir S, Okumus V, Ulutas MS, Dundar A, Akarsubası AT, Dumonted S (2015) Isolation of a novel thermophilic Anoxybacillus flavithermus SO- 13, production, characterization and industrial applications of its thermostable α-amylase. J Bioprocess Biotech 5(7):1–9. https://doi.org/10.4172/2155-9821.1000237

    Article  CAS  Google Scholar 

  • Özdemir S, Kılınç E, Poli A, Nicolaus B (2013) Biosorption of heavy metals (Cd2+, Cu2+, Co2+, and Mn2+) by thermophilic bacteria, Geobacillus thermantarcticus and Anoxybacillus amylolyticus: equilibrium and kinetic studies. Bioremed J 17(2):86–96

    Article  CAS  Google Scholar 

  • Padilha I, Carvalho L, Dias P, Grisi T, Silva F, Santos S, Araujo D (2015) Production and characterization of thermophilic carboxymethyl cellulase synthesized by Bacillus sp. growing on sugarcane bagasse in submerged fermentation. Braz J Chem Eng 32(1):35–42. https://doi.org/10.1590/0104-6632.20150321s00003298

    Article  CAS  Google Scholar 

  • Panosyan HH (2017) Thermophilic bacilli isolated from Armenian geothermal springs and their potential for production of hydrolytic enzymes. Int J Biotech Bioeng 3(8):239–244

    Google Scholar 

  • Panosyan H (2010) Phylogenetic diversity based on 16S rRNA gene sequence analysis of aerobic thermophilic endospore-forming bacteria isolated from geothermal springs in Armenia. Biol J Armenia 4(65):73–80

    Google Scholar 

  • Panosyan H, Birkeland NK (2014) Microbial diversity in an Armenian geothermal spring assessed by molecular and culture-based methods. J Basic Microbiol 54(11):1240–1250

    Article  CAS  PubMed  Google Scholar 

  • Panosyan H, Anzelmo G, Nicolaus B (2014) Production and characterization of exopolysaccharadies synthesized by geobacilli isolated from an Armenian geothermal spring. FEBS J 281(1):667

    Google Scholar 

  • Pikuta E, Cleland D, Tang J (2003) Aerobic growth of Anoxybacillus pushchinoensis K1(T): emended descriptions of A. pushchinoensis and the genus Anoxybacillus. Int J Syst Evol Microbiol 53(Pt 5):1561–1562

    Article  PubMed  CAS  Google Scholar 

  • Pikuta E, Lysenko A, Chuvilskaya N, Mendrock U, Hippe H, Suzina N, Nikitin D, Osipov G, Laurinavichius K (2000) Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov. Int J Syst Evol Microbiol 50(Pt 6):2109–2117

    Article  PubMed  CAS  Google Scholar 

  • Pinzon-Martinez DL, Rodriguez-Gomez C, Minana-Galbis D, Carrillo-Chavez JA, Valerio-Alfaro G, Oliart-Ros R (2010) Thermophilic bacteria from Mexican thermal environments: isolation and potential applications. Environ Technol 31(8-9):957–966

    Article  PubMed  CAS  Google Scholar 

  • Poli A, Guven K, Romano I, Pirinccioglu H, Guven RG, Euzeby JP, Matpan F, Acer O, Orlando P, Nicolaus B (2012) Geobacillus subterraneus subsp. aromaticivorans subsp. nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey. J Gen Appl Microbiol 58(6):437–446

    Article  PubMed  CAS  Google Scholar 

  • Poli A, Romano I, Cordella P, Orlando P, Nicolaus B, Ceschi Berrini C (2009) Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy. Extremophiles 13(6):867–874

    Article  PubMed  Google Scholar 

  • Poli A, Romano I, Caliendo G, Nicolaus G, Orlando P, Falco A, Lama L, Gambacorta A, Nicolaus B (2006) Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost. J Gen Appl Microbiol 52:223–234

    Article  PubMed  CAS  Google Scholar 

  • Priya I, Dhar MK, Bajaj BK, Koul S, Vakhlu J (2016) Cellulolytic activity of thermophilic bacilli isolated from Tattapani hot spring sediment in North West Himalayas. Indian J Microbiol 56(2):228–231. https://doi.org/10.1007/s12088-016-0578-4

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Radchenkova N, Vassilev S, Panchev I, Anzelmo G, Tomova I, Nicolaus B, Kuncheva M, Petrov K, Kambourova M (2013) Production and properties of two novel exopolysaccharides synthesized by a thermophilic bacterium Aeribacillus pallidus 418. Appl Biochem Biotechnol 171(1):31–43

    Article  PubMed  CAS  Google Scholar 

  • Rao MB, Tanksale AM, Ghatge MS, Deshpande VV (1998) Molecular and biotechnological aspects of microbial proteases. Microbiol Mol Biol Rev 62(3):597–635

    PubMed  PubMed Central  CAS  Google Scholar 

  • Reddy NS, Nimmagadda A, Sambasiva Rao KRS (2003) An overview of the microbial α-amylase family. Afr J Biotechnol 2:645–648

    Article  CAS  Google Scholar 

  • Rekadwad BN (2015) Characterization of amylase from industrially important thermophilic microorganism: Geobacillus thermoleovorans strain Rekadwadsis. Int J Life Sci Biotechnol Pharma Res 4(1):26–30

    CAS  Google Scholar 

  • Rozanov AS, Logacheva MD, Peltek SE (2014) Draft genome sequences of Geobacillus stearothermophilus strains 22 and 53, isolated from the garga hot spring in the Barguzin River Valley of the Russian Federation. Genome Announc 2(6):e01205-14

    Article  PubMed  PubMed Central  Google Scholar 

  • Satapathy SS, Dutta M, Ray SK (2010) Higher tRNA diversity in thermophilic bacteria: a possible adaptation to growth at high temperature. Microbiol Res 165(8):609–616

    Article  PubMed  CAS  Google Scholar 

  • Satyanarayana T, Sharma A, Mehta D, Puri AK, Kumar V, Nisha M, Joshi S (2012) Biotechnological applications of biocatalysts from the firmicutes Bacillus and Geobacillus species. In: Satyanarayana T, Johri BN (eds) Microorganisms in sustainable agriculture and biotechnology. Springer, Dordrecht, pp 343–379

    Chapter  Google Scholar 

  • Sawle L, Ghosh K (2011) How do thermophilic proteins and proteomes withstand high temperature? Biophys J 101(1):217–227

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Seifzadeh S, Hassan Sajedi R, Sariri R (2008) Isolation and characterization of thermophilic alkaline proteases resistant to sodium dodecyl sulfate and ethylene diamine tetraacetic acid from Bacillus sp. GUS1. Iran J Biotechnol 6(4):214–221

    CAS  Google Scholar 

  • Scandurra R, Consalvi V, Chiaraluce R, Politi L, Engel PC (1998) Protein thermostability in extremophiles. Biochimie 80(11):933–941

    Article  PubMed  CAS  Google Scholar 

  • Schäffer C, Franck WL, Scheberl A, Kosma P, McDermott TR, Messner P (2004) Classification of isolates from locations in Austria and Yellowstone National Park as Geobacillus tepidamans sp. nov. Int J Syst Evol Microbiol 54(Pt 6):2361–2238

    Article  PubMed  CAS  Google Scholar 

  • Schneider D, Liu Y, Gerstein M, Engelman DM (2002) Thermostability of membrane protein helix-helix interaction elucidated by statistical analysis. FEBS Lett 532:231–236

    Article  PubMed  CAS  Google Scholar 

  • Scholz T, Demharter W, Hensel R, Kandler O (1987) Bacillus pallidus sp. nov., a new thermophilic species from sewage. Syst Appl Microbiol 9:91–96

    Article  CAS  Google Scholar 

  • Shahinyan G (2015) Temperature and pH effect on lipase production and enzymatic activity of thermophilic bacilli isolated from Armenian geothermal springs. Sci Med J 10(1):9–15

    Google Scholar 

  • Shahinyan G, Margaryan A, Panosyan H, Trchounian A (2017) Identification and sequence analyses of novel lipase encoding novel thermophillic bacilli isolated from Armenian geothermal springs. BMC Microbiol 17(103). https://doi.org/10.1186/s12866-017-1016-4

  • Sharma A, Pandey A, Shouche YS, Kumar B, Kulkarni G (2009) Characterization and identification of Geobacillus spp. isolated from Soldhar hot spring site of Garhwal Himalaya, India. J Basic Microbiol 49(2):187–194

    Article  PubMed  CAS  Google Scholar 

  • Sharma R, Thakur V, Sharma M and Birkeland N-K (2013) Biocatalysis through thermostable lipases: adding flavor to chemistry, In Satyanarayana, T., Littlechild, J. & Kawarabayasi, Y. (eds.) Thermophilic microbes in environmental and industrial biotechnology: DordrechtSpringer : 905-927

    Chapter  Google Scholar 

  • Sharma P, Gupta S, Sourirajan A, Dev K (2015) Characterization of extracellular thermophillic cellulase from thermophilic Geobacillus sp. isolated from Tattapani Hot spring of Himachal Pradesh, India. Int JAdv Biotechnol Res (IJBR) 6(3):433–442

    CAS  Google Scholar 

  • Sharma A, Adhikari S, Satyanarayana T (2007) Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans. World J Microbiol Biotechnol 23(4):483–490

    Article  CAS  Google Scholar 

  • Shih TW, Pan TM (2011) Stress responses of thermophilic Geobacillus sp. NTU 03 caused by heat and heat-induced stress. Microbiol Res 166(5):346–359

    Article  PubMed  CAS  Google Scholar 

  • Sookkheo B, Sinchaikul S, Thannan H, Thongprasong O, Phutrakul S, Chen ST (2002) Proteomic analysis of a thermostable superoxide dismutase from Bacillus stearothermophilus TLS33. Proteomics 2(9):1311–1315

    Article  PubMed  CAS  Google Scholar 

  • Stenesh J, McGowan GR (1977) DNA polymerase from mesophilic and thermophilic bacteria. I. Purification and properties of DNA polymerase from Bacillus licheniformis and Bacillus stearothermophilus. Biochim Biophys Acta 272:156–166

    Article  Google Scholar 

  • Sung MH, Kim H, Bae JW, Rhee SK, Jeon CO, Kim K, Kim JJ, Hong SP, Lee SG, Yoon JH, Park YH, Baek DH (2002) Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost. Int J Syst Evol Microbiol 52(Pt 6):2251–2255

    PubMed  CAS  Google Scholar 

  • Sunna A, Prowe SG, Stoffregen T, Antranikian G (1997) Characterization of the xylanases from the new isolated thermophilic xylan-degrading Bacillus thermoleovorans strain K-3d and Bacillus flavothermus strain LB3A. FEMS Microbiol Lett 148(2):209–216

    Article  PubMed  CAS  Google Scholar 

  • Sutherland IW (1999) Polysaccharases for microbial exopolysaccharides. Carbohydr Polym 38(4):319–328

    Article  CAS  Google Scholar 

  • Synowiecki J (2010) Some applications of thermophiles and their enzymes for protein processing. Afr J Biotechnol 9:7020–7025

    CAS  Google Scholar 

  • Takami H, Takaki Y, Chee GJ, Nishi S, Shimamura S, Suzuki H, Matsui S, Uchiyama I (2004) Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus. Nucleic Acids Res 21:6292–6303

    Article  CAS  Google Scholar 

  • Tan H Alam M (2010) sRNAs are involved in high temperature adaptation of Geobacillus thermoleovorans. EMBO Symposium Non-coding genome, At Heidelberg, Germany

    Google Scholar 

  • Tolner B, Poolman B, Konings WN (1997) Adaptation of microorganisms and their transport systems to high temperatures. Comp Biochem Physiol 118A:423–428

    Article  CAS  Google Scholar 

  • Tourney J, Ngwenya BT (2014) The role of bacterial extracellular polymeric substances in geomicrobiology. Chem Geol 386(Supplement C):115–132

    Article  CAS  Google Scholar 

  • Trent JD (1996) A review of acquired thermotolerance, heat-shock proteins, and molecular chaperones in archaea. FEMS Microbiol Rev 18(2-3):249–258

    Article  CAS  Google Scholar 

  • Trivedi S, Gehlot HS, Rao SR (2006) Protein thermostability in Archaea and Eubacteria. Genet Mol Res 5(4):816–827

    PubMed  CAS  Google Scholar 

  • Turner P, Mamo G, Karlsson EN (2007) Potential and utilization of thermophiles and thermostable enzymes in biorefining. Microbial Cell Fact 6(1):9. https://doi.org/10.1186/1475-2859-6-9

    Article  CAS  Google Scholar 

  • Uma Maheswar Rao JL, Satyanarayana T (2007) Improving production of hyperthermostable and high maltose-forming alpha-amylase by an extreme thermophile Geobacillus thermoleovorans using response surface methodology and its applications. Bioresour Technol 98(2):345–352

    Article  PubMed  CAS  Google Scholar 

  • van de Vossenberg JL, Ubbink-Kok T, Elferink MG, Driessen AJ, Konings WN (1995) Ion permeability of the cytoplasmic membrane limits the maximum growth temperature of bacteria and archaea. Mol Microbiol 18(5):925–932

    Article  PubMed  Google Scholar 

  • van Kranendonk MJ, Djokic T, Campbell KA, Deamer D, Damer B, Walter MR, Steller L, Ota T, Nakamura E, Tadbiri S (2017) An origin of life at terrestrial hot springs: support from early earth and implications for the search for life on mars. Astrobiology Science Conference 2017 (LPI Contrib. No. 1965)

    Google Scholar 

  • Vardanyan G, Margaryan A, Panosyan H (2015) Isolation and characterization of lipase-producing bacilli from Tatev geothermal spring. Collection of Scientific Articles of YSU SSS 1.1(4): 33–36

    Google Scholar 

  • Wang GX, Wang Y, Wu ZF, Jiang HF, Dong RQ, Li FY, Liu XL (2011a) Immunomodulatory effects of secondary metabolites from thermophilic Anoxybacillus kamchatkensis XA-1 on carp, Cyprinus carpio. Fish Shellfish Immunol 30(6):1331–1338

    Article  PubMed  CAS  Google Scholar 

  • Wang Q, Cen Z, Zhao J (2015) The survival mechanisms of thermophiles at high temperatures: an angle of omics. Physiology (Bethesda) 30(2):97–106

    CAS  Google Scholar 

  • Wang X, Gao R, Liao C, Meng Y, Tang X, Li J, Xu B, Huang Z (2011b) Isolation of Geobacillus sp. A27 and characterization of its α-amylase. Sci Technol Food Ind 32:151–155 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Wang W, Ma T, Zhang B, Yao N, Li M, Li G, Ma Z, Cheng J (2014) A novel mechanism of protein thermostability: a unique N-terminal domain confers heat resistance to Fe/Mn-SODs. Sci Rep 4(7284):1–9. https://doi.org/10.1038/srep07284

    Article  CAS  Google Scholar 

  • Weiner R, Langille S, Quintero E (1995) Structure, function and immunochemistry of bacterial exopolysaccharides. J Ind Microbiol 15:339–346

    Article  PubMed  CAS  Google Scholar 

  • Yang Z, Zhang Y, Shen T, **e Y, Mao Y, Ji C (2013) Cloning, expression and biochemical characterization of a novel, moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2. J Biosci Bioeng 115(2):133–137

    Article  PubMed  CAS  Google Scholar 

  • Yohandini H, Julinar, Muharni (2015) Isolation and phylogenetic analysis of thermophile community within Tanjung Sakti Hot Spring, South Sumatera, Indonesia. HAYATI J Biosci 22(3):143–148

    Article  Google Scholar 

  • Yumoto I, Hirota K, Kawahara T, Nodasaka Y, Okuyama H, Matsuyama H, Yokota Y, Nakajima K, Hoshino T (2004) Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka. Int J Syst Evol Microbiol 54(Pt 4):1239–1242

    Article  PubMed  CAS  Google Scholar 

  • Zahoor S, Javed M, Babarl M (2016) Characterization of a novel hydrolytic enzyme producing thermophilic bacterium isolated from the Hot Spring of Azad Kashmir-Pakistan. Braz Arch Biol Technol 56:e16150622. https://doi.org/10.1590/1678-4324-2016150662

    Article  CAS  Google Scholar 

  • Zeigler R (2001) Bacillus genetic stock center catalog of strains (7th edn), Volume 3: The genus Geobacillus: 11

    Google Scholar 

  • Zhang XQ, Zhang ZL, Wu N, Zhu XF, Wu M (2013) Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol 63(Pt 11):4064–4071

    Article  PubMed  CAS  Google Scholar 

  • Zhang CM, Huang XW, Pan WZ, Zhang J, Wei KB, Klenk HP, Tang SK, Li WJ, Zhang KQ (2011) Anoxybacillus tengchongensis sp. nov. and Anoxybacillus eryuanensis sp. nov., facultatively anaerobic, alkalitolerant bacteria from hot springs. Int J Syst Evol Microbiol 61(Pt 1):118–122

    Article  PubMed  CAS  Google Scholar 

  • Zhao S, Cao F, Zhang H, Zhang L, Zhang F, Liang X (2014) Structural characterization and biosorption of exopolysaccharides from Anoxybacillus sp. R4-33 isolated from radioactive radon hot spring. Appl Biochem Biotechnol 172(5):2732–2746

    Article  PubMed  CAS  Google Scholar 

  • Zhu W, Cha D, Cheng G, Peng Q, Shen P (2007) Purification and characterization of a thermostable protease from a newly isolated Geobacillus sp. YMTC 1049. Enzyme Microb Technol 40:1592–1597

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from the EURASIA Programme of the Norwegian Center for International Cooperation in Education (CPEA-2011/10081, CPEA-LT-2016/10095) and partially supported by the RA MES State Committee of Science, in the frames of the research project no. 15T-1F399, and the Armenian National Science and Education Fund based in New York, USA, to HP (ANSEF-NS-microbio 2493, 3362, and 4676).

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Authors and Affiliations

  1. Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Yerevan, Armenia

    Armine Margaryan, Pargev Hovhannisyan, Hovik Panosyan & Armen Trchounian

  2. Research Institute of Biology, Yerevan State University, Yerevan, Armenia

    Armine Margaryan, Grigor Shahinyan & Armen Trchounian

  3. Department of Biological Sciences, University of Bergen, Bergen, Norway

    Nils-Kåre Birkeland

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  1. Armine Margaryan
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  1. Faculty of Biology, National University of Uzbekistan, Tashkent, Uzbekistan

    Dilfuza Egamberdieva

  2. Department of Biology, University of Bergen, Bergen, Norway

    Nils-Kåre Birkeland

  3. Faculty of Biology, Yerevan State University, Yerevan, Armenia

    Hovik Panosyan

  4. School of Life Sciences, Sun Yat-Sen University, Guangzhou, China

    Wen-Jun Li

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© 2018 Springer Nature Singapore Pte Ltd.

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Margaryan, A., Shahinyan, G., Hovhannisyan, P., Panosyan, H., Birkeland, NK., Trchounian, A. (2018). Geobacillus and Anoxybacillus spp. from Terrestrial Geothermal Springs Worldwide: Diversity and Biotechnological Applications. In: Egamberdieva, D., Birkeland, NK., Panosyan, H., Li, WJ. (eds) Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications. Microorganisms for Sustainability, vol 8. Springer, Singapore. https://doi.org/10.1007/978-981-13-0329-6_5

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