SpringerLink
Log in

Cryopreservation of Gentianaceae: Trends and Applications

  • Chapter
  • First Online:
The Gentianaceae - Volume 2: Biotechnology and Applications

Abstract

Cryopreservation technologies have opened the door to the possibility of long-term storage of valuable biodiverse germplasm of many plant species. However, for successful conservation by cryopreservation , a large amount of work is still needed. This chapter describes the role of plants belonging to the family Gentianaceae in improving our understanding of cryopreservation. Emphasis is on expected problems and benefits originated from enhancing embryogenic capacity by osmotic dehydration stress. In addition, routine application is shown of cryopreservation for long-term conservation of in vitro propagated germplasm of the Gentianaceae.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (Canada)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (Canada)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 109.99
Price excludes VAT (Canada)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Aguilar ME, Engelmann F, Michaux-Ferrière N (1993) Cryopreservation of cell suspensions of Citrus deliciosa Tan. and histological study. CryoLetters 14:217–228

    Google Scholar 

  • Arora R, Wisniewski ME (1995) Ultrastructural and protein changes in cell suspension cultures of peach associated with low temperature-induced cold acclimation and abscisic acid treatment. Plant Cell Tiss Org Cult 40:17–24

    Article  CAS  Google Scholar 

  • Bachiri Y, Bajon C, Sauvanet A, Gazeau C, Morisset C (2000) Effect of osmotic stress on tolerance of air-drying and cryopreservation of Arabidopsis thaliana suspension cells. Protoplasma 214:227–243

    Article  CAS  Google Scholar 

  • Burch J (2003) Some mosses survive cryopreservation without prior pretreatment. Bryologist 106:270–277

    Article  Google Scholar 

  • Carpentier S, Witters E, Laukens K, Onckelen HV, Swennen R, Panis B (2007) Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress. Proteomics 7:92–105

    Article  CAS  PubMed  Google Scholar 

  • Cho Ji-S, Hong S-M, Joo S-Y, Ji-S Yoo, Kim D-I (2007) Cryopreservation of transgenic rice suspension cells producing recombinant hCTLA4Ig. Appl Microbiol Biotechnol 73:1470–1476

    Article  CAS  PubMed  Google Scholar 

  • Chen Y, Wang JH (2002) Cryopreservation of carrot (Daucus carota L.) cell suspensions and protoplasts by vitrification. CryoLetters 24:57–64

    CAS  Google Scholar 

  • COST Action 871 (2006) Cryopreservation of crop species in Europe. In: http://w3.cost.eu/fileadmin/domain_files/ABFS/Action_871/mou/871-e.pdf

  • Criel B, Hausman J-F, Oufir M, Swennen R, Panis B, Renaut J (2006) Proteome and sugar analysis of abiotic stress underlying cryopreservation in potato. Commun Agricult Appl Biol Sci 71:3–6

    CAS  Google Scholar 

  • Domżalska L, Kędracka-Krok S, Rybczyński JJ (2011) Proteomic changes in Gentiana cruciata (L.) cell suspension during adaptation to osmotic stress in cryopreservation protocol. In: Pukacki PM (ed) Proceedings of the 17th cold hardiness seminar in Poland 2011, Institute of Dendrology PAS, pp 44–52

    Google Scholar 

  • Engelmann F (2004) Plant cryopreservation: progress and prospects. In Vitro Cell Dev Biol-Plant 40:427–433

    Article  Google Scholar 

  • Engelmann F (2011) Cryopreservation of embryos: an overview. In: Thorpe TA, Yeung EC (eds) Methods in molecular biology 710. Plant embryo culture. Methods and protocols. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada. Humana Press, Springer, Heidelberg, pp 155–184. doi:10.1007/978-1-61737-988-8

  • Fiuk A, Rybczyński JJ (2007) The effect of several factors on somatic embryogenesis and plant regeneration in protoplast cultures of Gentiana kurroo (Royle). Plant Cell Tiss Org Cult 91:263–271

    Article  Google Scholar 

  • Fiuk A, Rybczyński JJ (2008) Morphogenic capability of Gentiana kurroo Royle seedling and leaf explants. Acta Physiol Plant 30:157–166

    Article  Google Scholar 

  • Gnanapragasam S, Vasil IK (1992) Ultrastructural changes in suspension culture cells of Panicum maximum during cryopreservation. Plant Cell Rep 11:169–174

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez-Arnao MT, Engelmann F (2006) Cryopreservation of plant germplasm using the encapsulation-dehydration technique: review and case study on sugarcane. CryoLetters 27:155–168

    CAS  PubMed  Google Scholar 

  • González-Benito ME, Pérez C (1994) Studies on the cryopreservation of nodal explants of Centaurium rigualii Esteve, an endemic threatened species, through vitrification. Bot Gard Microprop News 1:82–84

    Google Scholar 

  • González-Benito ME, Pérez C, Viviani AB (1997) Cryopreservation of nodal explants of an endangered plant species (Centaurium rigualii Esteve) using the encapsulation-dehydration method. Biodivers Conserv 6:583–590

    Article  Google Scholar 

  • Häggman HM, Ryynänen LA, Aronen TS, Krajnakowa J (1998) Cryopreservation of embryogenic cultures of Scots pine. Plant Cell Tiss Org Cult 54:45–53

    Article  Google Scholar 

  • Hikage T, Saitoh Y, Tanaka-Saitoh C, Hagami H, Satou F, Shimotai Y, Nakano Y, Takahashi M, Takahata Y, Tsutsumi K (2007) Structure and allele-specific expression variation of novel α/β hydrolase fold proteins in gentian plants. Mol Genet Genomics 278:95–104

    Article  CAS  PubMed  Google Scholar 

  • Hikage T, Kogusuri K, Tanaka-Saito C, Watanabe S, Chiba S, Kume KH, Saitoh Y, Takahata Y, Tsutsumi K (2011) W14/15 esterase gene haplotype can be a genetic landmark of cultivars and species of the genus Gentiana L. Mol Genet Genomics 285:47–56

    Article  CAS  PubMed  Google Scholar 

  • Hitmi A, Coudret A, Barthomeuf C, Sallanon H (1999) The role of sucrose in freezing tolerance in Chrysanthemum cinerariaefolium L. cell cultures. CryoLetters 20:45–54

    CAS  Google Scholar 

  • Kamada H, Ishikawa K, Saga H, Harada H (1993) Induction of somatic embryogenesis in carrot by osmotic stress. Plant Tiss Cult Lett 10:38–44

    Article  CAS  Google Scholar 

  • Kikuchi A, Sanuki N, Higashi K, Koshiba T, Kamada H (2006) Abscisic acid and stress treatment are essential for acquisition of embryogenic competence by carrot cells. Planta 223:637–645

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi T, Niino T, Kobayashi M (2005) Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures. Plant Biotech 22:105–112

    Article  CAS  Google Scholar 

  • Menges M, Murray JA (2004) Cryopreservation of transformed and wild-type Arabidopsis and tobacco cell suspension cultures. Plant J 37:635–644

    Article  CAS  PubMed  Google Scholar 

  • Mikuła A (2006) Comparison of three techniques for cryopreservation and re-establishment of long-term Gentiana tibetica suspension culture. CryoLetters 27:268–282

    Google Scholar 

  • Mikuła A, Fiuk A, Rybczyński JJ (2005a) Induction, maintenance and preservation of embryogenic competence of Gentiana cruciata L. cultures. Acta Biol Crac Series Botanica 47:227–236

    Google Scholar 

  • Mikuła A, Rybczyński JJ, Skierski J, Latkowska MJ, Fiuk A (2005b) Somatic embryogenesis of Gentiana genus IV: characterization of Gentiana cruciata and Gentiana tibetica embryogenic cell suspensions. In: Hvoslef-Eide AK, Preis W (eds) Liquid culture systems for in vitro plant propagation. Springer, Berlin, pp 345–358

    Chapter  Google Scholar 

  • Mikuła A, Tykarska T, Kuraś M (2005c) Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters 26:367–378

    PubMed  Google Scholar 

  • Mikuła A, Niedzielski M, Rybczyński JJ (2006) The use of TTC reduction assay for assessment of Gentiana spp. cell suspension viability after cryopreservation. Acta Physiol Plant 28:315–324

    Article  Google Scholar 

  • Mikuła A, Olas M, Śliwińska E, Rybczyński JJ (2008) Cryopreservation by encapsulation of Gentiana spp. cell suspensions maintains re-growth, embryogenic competence and DNA content. CryoLetters 29:409–418

    PubMed  Google Scholar 

  • Mikuła A, Tomiczak K, Rybczyński JJ (2011a) Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants. Plant Cell Rep 30:565–574

    Article  PubMed Central  PubMed  Google Scholar 

  • Mikuła A, Tomiczak K, Wójcik A, Rybczyński JJ (2011b) Encapsulation-dehydration method of cryopreservation elevates embryogenesity of Gentiana kurroo cell suspension and carrying on genetic stability of its regenerants after cryopreservation. Acta Horticult 908:143–154

    Google Scholar 

  • Panis B, Strosse H, Hende SVD, Swennen R (2002) Sucrose preculture to simplify cryopreservation of banana meristem cultures. CryoLetters 23:375–384

    CAS  PubMed  Google Scholar 

  • Rybczyński JJ, Mikuła A, Tomiczak K, Wójcik A, Fiuk A (2008) Biotechnology of gentians: the induction of embryogenic potential, its cryopreservation and utilization in somatic cell genetic manipulation. Physiol Plant 133:P07–P055

    Google Scholar 

  • Sadia B, Anthony P, Lowe KC, Power JB, Davey MR (2003) Culture treatments for enhancing post-thaw recovery of cryopreserved suspension cells of potato cv. Desiree. Cell Mol Biol 8:979–989

    Google Scholar 

  • Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33

    Article  CAS  PubMed  Google Scholar 

  • Škrlep K, Bergant M, de Winter GM, Bohanec B, Žel J, Verpoorte R, van Iren F, Camloh M (2008) Cryopreservation of cell suspension cultures of Taxus × media and Taxus floridana. Biol Plant 52:329–333

    Article  Google Scholar 

  • Sarkar D, Naik PS (1998) Cryopreservation of shoot tips of tetraploid potato (Solanum tuberosum L.) clones by vitrification. Ann Bot 82:455–461

    Article  Google Scholar 

  • Suhartono L, Iren F, Winter W, Roytrakul S, Choi YH, Verpoorte R (2005) Metabolic comparison of cryopreserved and normal cells from Tabernaemontana divaricata suspension cultures. Plant Cell Tiss Org Cult 83:59–66

    Article  CAS  Google Scholar 

  • Suzuki M, Ishikawa M, Akihama T (1998) A novel preculture method for the induction of desiccation tolerance in gentian axillary buds for cryopreservation. Plant Sci 135:69–76

    Article  CAS  Google Scholar 

  • Suzuki M, Akihama T, Ishikawa M (2005) Cryopreservation of encapsulation gentian axillary buds following 2 step-preculture with sucrose and desiccation. Plant Cell Tiss Org Cult 83:115–121

    Article  CAS  Google Scholar 

  • Suzuki M, Ishikawa M, Okuda H, Noda K, Kishimoto T, Nakamura T, Ogiwara I, Shimura I, Akihama T (2006) Physiological changes in gentian axillary buds during two-step preculturing with sucrose that conferred high levels of tolerance to desiccation and cryopreservation. Ann Bot 97:1073–1081

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Suzuki M, Tandon P, Ishikawa M, Toyomasu T (2008) Development of a new vitrification solution, VSL, and its application to the cryopreservation of gentian axillary buds. Plant Biotechnol Rep 2:123–131

    Article  Google Scholar 

  • Takahashi M, Hikage T, Yamashita T, Saitoh Y, Endou M, Tsutsumi K (2006) Stress-related proteins are specifically expressed under non-stress conditions in the overwinter buds of the gentian plant Gentiana trifora. Breed Sci 56:39–46

    Article  CAS  Google Scholar 

  • Tanaka D, Niino T, Isuzugawa K, Hikage T, Uemura M (2004) Cryopreservation of shoot apices of in vitro grown gentian plants: comparison of vitrification and encapsulation-vitrification protocols. CryoLetters 25:167–176

    PubMed  Google Scholar 

  • Towill LE, Forsline PL, Walters C, Waddell JW, Laufmann J (2004) Cryopreservation of Malus germplasm using a winter vegetative bud method: results from 1915 accessions. CryoLetters 25:323–334

    PubMed  Google Scholar 

  • Wang ZY, Legris G, Nagel J, Potrykus I, Spangenberg G (1994) Cryopreservation of embryogenic cell suspensions in Festuca and Lolium species. Plant Sci 103:93–106

    Article  CAS  Google Scholar 

  • Wang JH, Ge JG, Liu F, Huang CN (1998) Ultrastructural changes during cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells by vitrification. CryoLetters 19:49–54

    Google Scholar 

  • Wang Q, Gafny R, Sahar N, Sela I, Mawassi M, Tanne E, Perl A (2002) Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Sci 162:551–558

    Article  CAS  Google Scholar 

  • Wang W, Scali M, Vignani R, Spadafora A, Sensi E, Mazzuca S, Cresti M (2003) Protein extraction for two-dimension electrophoresis from olive leaf, a plant tissue containing high level of interfering compounds. Electrophoresis 24:2369–2375

    Article  CAS  PubMed  Google Scholar 

  • Winkelmann T, Muβmann V, Serek M (2004) Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill. Plant Cell Rep 23:1–8

    Article  CAS  PubMed  Google Scholar 

  • Wu Y-J, Huang X-L, Chen Q-Z, Li X-J, Engelmann F (2007) Induction and cryopreservation of embryogenic cultures from nucelli and immature cotyledon cuts of mango (Mangifera indica L. var Zihua). Plant Cell Rep 26:161–168

    Article  CAS  PubMed  Google Scholar 

  • Xue SH, Luo XJ, Wu ZH, Zhang HL, Wang XY (2008) Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall. Plant Cell Tiss Org Cult 92:251–260

    Article  Google Scholar 

  • Zhang YX, Wang JH, Bian HW, Zhu MY (2001) Pregrowth-desiccation: a simple efficient procedure for the cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells. CryoLetters 22:221–228

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Polish Academy of Sciences, Botanical Garden—Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland

    Anna Mikuła, Karolina Tomiczak, Lucyna Domżalska & Jan J. Rybczyński

Authors
  1. Anna Mikuła
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karolina Tomiczak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lucyna Domżalska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jan J. Rybczyński
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anna Mikuła .

Editor information

Editors and Affiliations

  1. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Jan J. Rybczyński

  2. Plant and Crop Sciences Division, University of Nottingham School of Biosciences, Loughborough, United Kingdom

    Michael R. Davey

  3. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Anna Mikuła

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Mikuła, A., Tomiczak, K., Domżalska, L., Rybczyński, J.J. (2015). Cryopreservation of Gentianaceae: Trends and Applications. In: Rybczyński, J., Davey, M., Mikuła, A. (eds) The Gentianaceae - Volume 2: Biotechnology and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54102-5_11

Download citation

Publish with us

Policies and ethics

Search

Navigation