Abstract
Quantifying concentrations of target molecules near cellular structures, within cells or tissues, requires identifying the gold particles in immunogold labelled images. In this paper, we address the problem of automatically detect them accurately and reliably across multiple scales and in noisy conditions. For this purpose, we introduce a new contrast filter, based on an adaptive version of the H-extrema algorithm. The filtered images are simplified with a geodesic reconstruction to precisely segment the candidates. Once the images are segmented, we extract classical features and then classify using the majority vote of multiple classifiers. We characterize our algorithm on a pilot data and present results that demonstrate its effectiveness.
Chapter PDF
Similar content being viewed by others
References
Serra, J.: Image Analysis and Mathematical Morphology. Academic Press, London (1982)
Grimaud, M.: New measure of contrast: the dynamics. In: Paul, D., Gader; Edward, R., Dougherty; Jean, C. (eds.) Proc. SPIE, Image Algebra and Morphological Image Processing III, vol. 1769, pp. 292–305 (1992)
Thibault, G., Fertil, B., Navarro, C., Pereira, S., Cau, P., Levy, N., Sequeira, J., Mari, J.-L.: Shape and Texture Indexes: Application to Cell Nuclei Classification. International Journal of Pattern Recognition and Artificial Intelligence (IJPRAI) 27(1) (2013)
Jan, W., Slot, H.J.: Geuze: Cryosectioning and immunolabeling. Nature 10, 2480–2491 (2007)
Berkson, J.: Application of the Logistic Function to Bio-Assay. Journal of the American Statistical Association 39 (1944)
Breiman, L.: Random forests. Machine Learning 45(1), 5–32 (2001)
McCulloch, W.S., Pitts, W.: A logical calculus of the ideas immanent in nervous activity. Bulletin of Mathematical Biophysics, 115–133 (1944)
Karreman, M.A., van Donselaar, E.G., Gerritsen, H.C., Verrips, C.T., Verkleij, A.J.: VIS2FIX: A high-speed fixation method for immuno-electron microscopy. Traffic 12(7), 806–814 (2011)
Karreman, M.A., Agronskaia, A.V., van Donselaar, E.G., Vocking, K., Fereidouni, F., Humbel, B.M., Verrips, C.T., Verkleij, A.J., Gerritsen, H.C.: Optimizing immuno-labeling for correlative fluorescence and electron microscopy on a single specimen. Journal of Structural Biology (2012)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Thibault, G., Il**, K., Arthur, C., Shafran, I., Gray, J. (2013). Adaptive H-Extrema for Automatic Immunogold Particle Detection. In: Ruiz-Shulcloper, J., Sanniti di Baja, G. (eds) Progress in Pattern Recognition, Image Analysis, Computer Vision, and Applications. CIARP 2013. Lecture Notes in Computer Science, vol 8259. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-41827-3_30
Download citation
DOI: https://doi.org/10.1007/978-3-642-41827-3_30
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-41826-6
Online ISBN: 978-3-642-41827-3
eBook Packages: Computer ScienceComputer Science (R0)