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Effects of Fluoroquinolone Exposure to Growth and Morphology in Staphylococcus aureus

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9th International Conference on the Development of Biomedical Engineering in Vietnam (BME 2022)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 95))

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Abstract

Under exposure to fluoroquinolone (FQ) stressors, Staphylococcus aureus generates resistance through adaptive responses via alterations of target enzymes, efflux pumps, morphology, or general gene expression and mutation. This study examined the effects of continuous FQs exposure on the growth and morphology of bacterial colonies and cells. S. aureus ATCC 29213 and six FQ-exposed strains, which were induced through in vitro exposing S. aureus ATCC 29213 to sub-MIC value of ciprofloxacin (CIP), ofloxacin (OFL), and levofloxacin (LEV) were included in the study. Growth of these seven S. aureus strains in Luria-Bertani broth (LB), Mueller-Hinton broth (MHB), and Nutrient broth (NB) was investigated using plate counting method while light microscope and scanning electron microscope were used to identify changes in colony and cell morphology. Regarding growth analysis, most FQ-exposed S. aureus showed significantly lower growth rates in LB and MHB but higher in NB compared to the initial strain. Besides, nearly all FQ-exposed S. aureus experienced a decrease by about 28% in normal-colony perimeter and attained a roughly 22% increase in single-cell size compared to the initial strain. The percentage of small colony variants (SCV) of most FQ-exposed strains increased compared to the initial S. aureus. This study suggested that the adaptive response mechanisms to FQ exposure are associated with changes in the bacterial metabolism which resulted in a decrease of growth in nutrient-rich medium but an increase in nutrient-less one. Besides, S. aureus decreased its normal colony perimeter but increased the SCV and single-cell size percentage under serial exposure to FQs.

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Acknowledgment

The research topic was supported by The Youth Incubator for Science and Technology Programme, managed by Youth Promotion Science and Technology Center - Ho Chi Minh Communist Youth Union and Department of Science and Technology of Ho Chi Minh City, the contract number is “34/2022/ HĐ-KHCNT-VU” signed on 30th, December 2022.

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Authors and Affiliations

  1. School of Biotechnology, International University, Ho Chi Minh City, Vietnam

    Van Nhi Tran, Minh Khang Tran, Thuc Quyen Huynh & Thi Thu Hoai Nguyen

  2. Research Center for Infectious Diseases, International University, Ho Chi Minh City, Vietnam

    Thuc Quyen Huynh & Thi Thu Hoai Nguyen

  3. Vietnam National University, Ho Chi Minh City, Vietnam

    Van Nhi Tran, Minh Khang Tran, Thuc Quyen Huynh & Thi Thu Hoai Nguyen

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  1. Van Nhi Tran
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  2. Minh Khang Tran
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  3. Thuc Quyen Huynh
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  4. Thi Thu Hoai Nguyen
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Correspondence to Thi Thu Hoai Nguyen .

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Editors and Affiliations

  1. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Van Toi Vo

  2. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Thi-Hiep Nguyen

  3. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Binh Long Vong

  4. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Ngoc Bich Le

  5. School of Biomedical Engineering, International University—Vietnam National University, Ho Chi Minh City, Vietnam

    Thanh Qua Nguyen

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Tran, V.N., Tran, M.K., Huynh, T.Q., Nguyen, T.T.H. (2024). Effects of Fluoroquinolone Exposure to Growth and Morphology in Staphylococcus aureus. In: Vo, V.T., Nguyen, TH., Vong, B.L., Le, N.B., Nguyen, T.Q. (eds) 9th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2022. IFMBE Proceedings, vol 95. Springer, Cham. https://doi.org/10.1007/978-3-031-44630-6_74

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  • DOI: https://doi.org/10.1007/978-3-031-44630-6_74

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-031-44629-0

  • Online ISBN: 978-3-031-44630-6

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