Abstract
mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling.
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Acknowledgements
This work was supported by National Institutes of Health Grant R01 GM090056 and American Cancer Society Grant RSG-12-186.
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Yao, C., Shi, Y. (2014). Global and Quantitative Profiling of Polyadenylated RNAs Using PAS-seq. In: Rorbach, J., Bobrowicz, A. (eds) Polyadenylation. Methods in Molecular Biology, vol 1125. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-971-0_16
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DOI: https://doi.org/10.1007/978-1-62703-971-0_16
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-971-0
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