Measurements of Cytosolic Ion Concentrations in Live Cells

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Plant Mineral Nutrients

Part of the book series: Methods in Molecular Biology ((MIMB,volume 953))

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Abstract

Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes. We describe procedures for the use of SBFI for the measurement of Na+ in live cells. The resulting material is suitable for most standard cell biology procedures.

Veena S. Anil, Kavitha P.G., and Sam Kuruvilla have contributed equally to this work.

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Correspondence to M. K. Mathew .

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Anil, V.S., Kavitha, P.G., Kuruvilla, S., Kumar, P., Mathew, M.K. (2013). Measurements of Cytosolic Ion Concentrations in Live Cells. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_15

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  • DOI: https://doi.org/10.1007/978-1-62703-152-3_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-151-6

  • Online ISBN: 978-1-62703-152-3

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