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Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

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Collagen

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1944))

Abstract

Chemical cross-linking of collagen-based devices is used as a means of increasing the mechanical stability and control the degradation rate upon implantation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC; carboxyl terminal cross-linker) collagen films. In addition, we provide protocols to characterize the biophysical (swelling), biomechanical (tensile), and biological (metabolic activity, proliferation and viability using human dermal fibroblasts and THP-1 macrophages) properties of the cross-linked collagen scaffolds.

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Acknowledgments

This work has been supported by the Science Foundation Ireland, Career Development Award Programme (grant agreement number: 15/CDA/3629); Science Foundation Ireland and the European Regional Development Fund (grant agreement number: 13/RC/2073); and EU H2020, ITN award, Tendon Therapy Train Project (grant agreement number: 676338).

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Authors and Affiliations

  1. Regenerative, Modular and Developmental Engineering Laboratory (REMODEL), National University of Ireland Galway (NUI Galway), Galway, Ireland

    Ignacio Sallent, Héctor Capella-Monsonís & Dimitrios I. Zeugolis

  2. Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway (NUI Galway), Galway, Ireland

    Ignacio Sallent, Héctor Capella-Monsonís & Dimitrios I. Zeugolis

Authors
  1. Ignacio Sallent
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  2. Héctor Capella-Monsonís
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  3. Dimitrios I. Zeugolis
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Corresponding author

Correspondence to Dimitrios I. Zeugolis .

Editor information

Editors and Affiliations

  1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Irit Sagi

  2. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Nikolaos A. Afratis

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Sallent, I., Capella-Monsonís, H., Zeugolis, D.I. (2019). Production and Characterization of Chemically Cross-Linked Collagen Scaffolds. In: Sagi, I., Afratis, N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9095-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9095-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9094-8

  • Online ISBN: 978-1-4939-9095-5

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