Abstract
Here in we describe a solid phase synthesis of oligonucleotides bearing unnatural moiety appropriate for complex formation with In111 as well as their deprotection, isolation, and purification. We also present methods for oligonucleotides/In111 complex formulation with single and double stranded oligonucleotides of RNA nature and give an example of preparation method for one supramolecular drug delivery system (DDS) consisting of radiolabeled siRNA and positively charged peptide.
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Antopolsky, M. (2019). Synthetic Oligonucleotides in SPECT/CT In Vivo Imaging: Chemical Modifications, In111 Complex Formation, Incorporation into Drug Delivery Systems. In: Ogris, M., Sami, H. (eds) Nanotechnology for Nucleic Acid Delivery. Methods in Molecular Biology, vol 1943. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9092-4_5
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DOI: https://doi.org/10.1007/978-1-4939-9092-4_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9091-7
Online ISBN: 978-1-4939-9092-4
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