SpringerLink
Log in

Transmission Electron Microscopy of Bone

  • Protocol
  • First Online:
Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

  • 3536 Accesses

Abstract

Electron microscopic analysis of mineralized tissues like bone and dentin is essential for understanding of cell–cell/cell–matrix interactions, and the three-dimensional organization of these tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources for ultrastructural analysis by transmission electron microscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
EUR 44.95
Price includes VAT (France)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
EUR 181.89
Price includes VAT (France)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
EUR 232.09
Price includes VAT (France)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Goggin PM, Zygalakis KC, Oreffo ROC, Schneider P (2016) High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease. Eur Cell Mater 31:264–295

    Article  CAS  Google Scholar 

  2. Schwarcz HP (2015) The ultrastructure of bone as revealed in electron microscopy of ion-milled sections. Semin Cell Dev Biol 46:44–50

    Article  Google Scholar 

  3. Miltić Ž, Stolić A, Stojanović S, Najman S, Ignjatović N, Nikolić G, Trajanović M (2017) Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: a review. Mat Sci Eng C Mater Biol Appl 79:930–949

    Article  Google Scholar 

  4. Gruber HE (1992) Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 67:30–34

    Article  CAS  Google Scholar 

  5. Rungby J, Kassem M, Eriksen EF, Danscher G (1993) The von Kossa reaction for calcium deposits: silver lactate staining increases sensitivity and reduces background. Hist J 25:446–451

    Article  CAS  Google Scholar 

  6. Reynolds ES (1963) The use of lead nitrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208

    Article  CAS  Google Scholar 

  7. Everts V, Korper W, Jansen DC, Steinfort J, Lammerse I, Heera S, Docherty AJP, Beertsen W (1999) Functional heterogeneity of osteoclasts: matrix metalloproteinases participate in osteoclastic resorption of calvarial bone but not in resorption of long bone. FASEB J 13:1219–1230

    Article  CAS  Google Scholar 

  8. Landis WJ, Paine MC, Glimcher MJ (1978) Use of acrolein vapors for anhydrous preparation of bone tissue for electron microscopy. J Ultrastruct Res 70:171–180

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, VU University Amsterdam, Amsterdam, The Netherlands

    Vincent Everts & Wouter Beertsen

  2. Research Institute MOVE, VU University Amsterdam, Amsterdam, The Netherlands

    Vincent Everts, Anneke Niehof & Wouter Beertsen

  3. Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam, Amsterdam, The Netherlands

    Anneke Niehof & Wouter Beertsen

  4. Department of Cell Biology and Histology, Academic Medical Centre (AMC), University of Amsterdam, Amsterdam, The Netherlands

    Wikky Tigchelaar-Gutter

Authors
  1. Vincent Everts
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anneke Niehof
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wikky Tigchelaar-Gutter
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wouter Beertsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vincent Everts .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Everts, V., Niehof, A., Tigchelaar-Gutter, W., Beertsen, W. (2019). Transmission Electron Microscopy of Bone. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_32

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_32

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation