Abstract
During C. elegans larval development, the Q neuroblasts produce their lineage by three rounds of divisions along with continuous cell migrations. Their neuronal progeny is dispersed from the pharynx to the anus. This in vivo system to study cell migration is appealing for several reasons. The lineage development is stereotyped; functional analysis and genomic screens are rendered easy and powerful thanks to powerful tools; transgenic manipulations and genome engineering are efficient and can be conveniently combined with live-cell imaging. Here we describe a series of protocols in Q cell migration studies, including quantifications of progeny position, genetic screening strategies, preparation of migration mutants or transgenic worms expressing related fluorescent proteins, multipositional time-lapse tracking of Q cell migration using confocal microscopy and image analyses of single cell movements and dynamics.
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References
Sulston JE, Horvitz HR (1977) Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev Biol 56(1):110–156
Chalfie M, Sulston J (1981) Developmental genetics of the mechanosensory neurons of Caenorhabditis elegans. Dev Biol 82(2):358–370
Ou G, Vale RD (2009) Molecular signatures of cell migration in C. elegans Q neuroblasts. J Cell Biol 185(1):77–85. https://doi.org/10.1083/jcb.200812077
Chapman JO, Li H, Lundquist EA (2008) The MIG-15 NIK kinase acts cell-autonomously in neuroblast polarization and migration in C. elegans. Dev Biol 324(2):245–257. https://doi.org/10.1016/j.ydbio.2008.09.014
Zinovyeva AY, Yamamoto Y, Sawa H, Forrester WC (2008) Complex network of Wnt signaling regulates neuronal migrations during Caenorhabditis elegans development. Genetics 179(3):1357–1371. https://doi.org/10.1534/genetics.108.090290
Dyer JO, Demarco RS, Lundquist EA (2010) Distinct roles of Rac GTPases and the UNC-73/Trio and PIX-1 Rac GTP exchange factors in neuroblast protrusion and migration in C. elegans. Small GTPases 1(1):44–61. https://doi.org/10.4161/sgtp.1.1.12991
Sundararajan L, Lundquist EA (2012) Transmembrane proteins UNC-40/DCC, PTP-3/LAR, and MIG-21 control anterior-posterior neuroblast migration with left-right functional asymmetry in Caenorhabditis elegans. Genetics 192(4):1373–1388. https://doi.org/10.1534/genetics.112.145706
Harris J, Honigberg L, Robinson N, Kenyon C (1996) Neuronal cell migration in C. elegans: regulation of Hox gene expression and cell position. Development 122(10):3117–3131
Maloof JN, Whangbo J, Harris JM, Jongeward GD, Kenyon C (1999) A Wnt signaling pathway controls hox gene expression and neuroblast migration in C. elegans. Development 126(1):37–49
Honigberg L, Kenyon C (2000) Establishment of left/right asymmetry in neuroblast migration by UNC-40/DCC, UNC-73/Trio and DPY-19 proteins in C. elegans. Development 127(21):4655–4668
Middelkoop TC, Korswagen HC (2014) Development and migration of the C. elegans Q neuroblasts and their descendants. WormBook:1–23. https://doi.org/10.1895/wormbook.1.173.1
Cheng Z, Yi P, Wang X, Chai Y, Feng G, Yang Y, Liang X, Zhu Z, Li W, Ou G (2013) Conditional targeted genome editing using somatically expressed TALENs in C. elegans. Nat Biotechnol 31(10):934–937. https://doi.org/10.1038/nbt.2674
Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W, Ou G (2014) Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development. Dev Cell 30(5):625–636. https://doi.org/10.1016/j.devcel.2014.07.017
Chai Y, Li W, Feng G, Yang Y, Wang X, Ou G (2012) Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development. Nat Protoc 7(12):2090–2102. https://doi.org/10.1038/nprot.2012.128
Tian D, Diao M, Jiang Y, Sun L, Zhang Y, Chen Z, Huang S, Ou G (2015) Anillin regulates neuronal migration and neurite growth by linking RhoG to the actin cytoskeleton. Curr Biol 25(9):1135–1145. https://doi.org/10.1016/j.cub.2015.02.072
Zhu Z, Chai Y, Jiang Y, Li W, Hu H, Li W, Wu JW, Wang ZX, Huang S, Ou G (2016) Functional coordination of WAVE and WASP in C. elegans neuroblast migration. Dev Cell 39(2):224–238. https://doi.org/10.1016/j.devcel.2016.09.029
Acknowledgements
This study was supported by the National Natural Science Foundation of China to G.O. and Y.C. (31671451, 31525015, 31561130153, and 31190063), the Junior Thousand Talents Program of China to G.O., and the National Basic Research Program of China to G.O. (973 Program, 2013CB945600).
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Chai, Y., Zhu, Z., Ou, G. (2018). Migration of Q Cells in Caenorhabditis elegans. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_18
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DOI: https://doi.org/10.1007/978-1-4939-7701-7_18
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