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Targeted Electrophysiological Recordings In Vivo in the Mouse Cerebellum

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Extracellular Recording Approaches

Part of the book series: Neuromethods ((NM,volume 134))

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Abstract

Single-unit recordings in vivo are the unitary elements in the processing of the brain and as such essential in systems physiology to understand brain functioning. In the cerebellum, a structure with high levels of intrinsic activity, studying these elements in vivo in an awake animal is imperative to obtain information regarding the processing features of these units in action. In this chapter we address the rationale and the approach of recording electrophysiological activity in the cerebellum, particularly that of Purkinje cells, in vivo in the awake, active animal. In line with the develo** appreciation for the diversity within populations of the cells of the same type, there is a growing interest in the differentiation within the population of Purkinje cells. Here we describe a successful approach to analyzing the activity of two populations of Purkinje cells, which differ in connectivity and the expression of several genes. By driving the expression of a fluorescent marker with the promotor of one of the differentiating genes, the presence of a fluorescence signal could be used to recognize and approach Purkinje cells, while the intensity of the signal can be used as a marker to identify the two subpopulations. Finally, the drawbacks and the advantages of this technique are discussed and placed into a future perspective.

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Authors and Affiliations

  1. Department of Neuroscience, Erasmus Medical Center, GE, Rotterdam, The Netherlands

    Bin Wu & Martijn Schonewille

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  1. Bin Wu
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  2. Martijn Schonewille
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Correspondence to Martijn Schonewille .

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  1. Department of Pathology & Immunology and Neuroscience, Baylor College of Medicine, Houston, Texas, USA

    Roy V. Sillitoe

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Wu, B., Schonewille, M. (2018). Targeted Electrophysiological Recordings In Vivo in the Mouse Cerebellum. In: Sillitoe, R. (eds) Extracellular Recording Approaches. Neuromethods, vol 134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7549-5_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7549-5_2

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