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In Vivo Determination of Glutamate Uptake by Brain Microdialysis

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Biochemical Approaches for Glutamatergic Neurotransmission

Part of the book series: Neuromethods ((NM,volume 130))

Abstract

Glutamate uptake has a predominant role in the regulation of glutamate homeostasis mainly for the prevention of hyperexcitability and excitotoxicity which induce neuronal dysfunctions and neurological diseases. An evaluation of in vivo glutamate transport can enable to verify the functional impact of alterations previously found in in vitro and ex vivo preparations. The use of brain microdialysis to sample the extracellular medium has allowed to quantify the dynamic uptake of glutamate through the active transporters present on cell membranes which maintain glutamate homeostasis. We describe two quantitative methods based on the application of labeled or unlabeled glutamate through a microdialysis probe to measure the in vivo uptake of glutamate in a discrete brain area of freely moving rats, accompanied with step by step details. Advantages and drawbacks of the methods are also discussed on both methodological and physiological bases, by relying on previous studies carried out in experimental pathological or pharmacological models.

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Acknowledgments

The authors thank the Institut National de la Santé et de la Recherche Médicale (INSERM), the Centre National de la Recherche Scientifique (CNRS), and the Université Claude Bernard Lyon 1.

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Authors and Affiliations

  1. CNRS UMR5292, INSERM U1028, University Lyon 1, Lyon Neuroscience Research Center, NeuroDialyTics Unit, 7 rue Guillaume Paradin, Lyon, 69372, France

    Sandrine Parrot & Luc Denoroy

  2. Lyon Neuroscience Research Center, INSERM U1028, CNRS UMR5292, Université Lyon 1, 7 rue Guillaume Paradin, Lyon, 69372, France

    Monique Touret

Authors
  1. Sandrine Parrot
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  2. Monique Touret
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  3. Luc Denoroy
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Corresponding author

Correspondence to Sandrine Parrot .

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Editors and Affiliations

  1. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Sandrine Parrot

  2. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Luc Denoroy

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Parrot, S., Touret, M., Denoroy, L. (2018). In Vivo Determination of Glutamate Uptake by Brain Microdialysis. In: Parrot, S., Denoroy, L. (eds) Biochemical Approaches for Glutamatergic Neurotransmission. Neuromethods, vol 130. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7228-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7228-9_14

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  • Print ISBN: 978-1-4939-7227-2

  • Online ISBN: 978-1-4939-7228-9

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