SpringerLink
Log in

Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

  • Protocol
  • First Online:
Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

  • 4205 Accesses

Abstract

Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Pro B, Dang NH (2004) CD26/dipeptidyl peptidase IV and its role in cancer. Histol Histopathol 19:1345–1351

    CAS  PubMed  Google Scholar 

  2. Ohnuma K, Hosono O, Dang NH, Morimoto C (2011) Dipeptidyl peptidase in autoimmune pathophysiology. Adv Clin Chem 53:51–84

    Article  CAS  PubMed  Google Scholar 

  3. Gorrell MD, Abbott CA, Kähne T et al (2000) Relating structure to function in the beta-propeller domain of dipeptidylpeptidase IV. Point mutations that influence adenosine deaminase binding, antibody binding and enzyme activity. In: Langner J, Ansorge S (eds) Cellular peptidases in immune functions and diseases 2. Kluwer, New York, NY, pp 89–95

    Google Scholar 

  4. Ohnuma K, Inoue H, Uchiyama M, Yamochi T, Hosono O, Dang NH, Morimoto C (2006) T-cell activation via CD26 and caveolin-1 in rheumatoid synovium. Mod Rheumatol 6(1):3–13

    Article  Google Scholar 

  5. Tarantola E, Bertone V, Milanesi G et al (2012) DipeptidylpeptidaseIV, a key enzyme for the degradation of incretins and neuropeptides: activity and expression in the liver of lean and obese rats. Eur J Histochem 56:e41

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Mentlein R (1999) Dipeptidyl-peptidase IV (CD26)-role in the inactivation of regulatory peptides. Regul Pept 85:9–24

    Article  CAS  PubMed  Google Scholar 

  7. Mentlein R (2009) Mechanisms underlying the rapid degradation and elimination of the incretin hormones GLP-1 and GIP. Best Pract Res Clin Endocrinol Metab 4:443–452

    Article  Google Scholar 

  8. Hildebrandt M, Rose M, Mayr C et al (2000) Dipeptidyl peptidase IV (DPP IV, CD26) in patients with mental eating disorders. Adv Exp Med Biol 477:197–204

    Article  CAS  PubMed  Google Scholar 

  9. Gorrell MD (2005) Dipeptidyl peptidase IV and related enzymes in cell biology and liver disorders. Clin Sci 108:1–16

    Article  Google Scholar 

  10. Itou M, Kawaguchi T, Taniguchi E, Sata M (2013) Dipeptidyl peptidase-4: a key player in chronic liver disease. World J Gastroenterol 19:2298–2306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Miyazaki M, Kato M, Tanaka K et al (2012) Increased hepatic expression of dipeptidyl peptidase-4 in non-alcoholic fatty liver disease and its association with insulin resistance and glucose metabolism. Mol Med Report 5:729–733

    CAS  Google Scholar 

  12. Williams KH, Shackel NA, Gorrell MD et al (2013) Diabetes and nonalcoholic fatty liver disease: a pathogenic duo. Endocr Rev 34:84–129

    Article  CAS  PubMed  Google Scholar 

  13. Kesty NC, Roth JD, Maggs D (2008) Hormone based therapies in the regulation of fuel metabolism and body weight. Expert Opin Biol Ther 8:1733–1747

    Article  CAS  PubMed  Google Scholar 

  14. Chyan YJ, Chuang LM (2007) Dipeptidyl peptidase-IV inhibitors: an evolving treatment for type 2 diabetes from the incretin concept. Recent Pat Endocr Metab Immun Drug Discov 1:15–24

    Article  CAS  Google Scholar 

  15. DiStefano JK, Watanabe RM (2010) Pharmacogenetics of anti-diabetes drugs. Pharmaceuticals (Basel) 3:2610–2646

    Article  CAS  Google Scholar 

  16. Martin JH, Deacon CF, Gorrell MD, Prins JB (2011) Incretin-based therapies-review of the physiology, pharmacology and emerging clinical experience. Intern Med J 41:299–307

    Article  CAS  PubMed  Google Scholar 

  17. Bergmann K, Sypniewska G (2013) Diabetes as a complication of adipose tissue dysfunction. Is there a role for potential new biomarkers? Clin Chem Lab Med 51:177–185

    Article  CAS  PubMed  Google Scholar 

  18. Piazza GA, Callanan HM, Mowery J et al (1989) Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix. Biochem J 262:327–334

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Balaban YH, Korkusuz P, Simsek H et al (2007) Dipeptidyl peptidase IV (DDP IV) in NASH patients. Ann Hepatol 6(4):242–250

    CAS  PubMed  Google Scholar 

  20. Wang XM, Yao TW, Nadvi NA et al (2008) Fibroblast activation protein and chronic liver disease. Front Biosci 13:3168–3180

    Article  CAS  PubMed  Google Scholar 

  21. Gorrell MD, Gysbers V, McCaughan GW (2001) CD26: a multifunctional integral membrane and secreted protein of activated lymphocytes. Scand J Immunol 54:249–264

    Article  CAS  PubMed  Google Scholar 

  22. Gonzalez-Gronow M, Hershfield MS, Arredondo-Vega FX et al (2001) Cell surface adenosine deaminase binds and stimulates plasminogen activation on 1-LN human prostate cancer cells. J Biol Chem 279:20993–20998

    Article  Google Scholar 

  23. Matheeussen V, Jungraithmayr W, De Meester I (2012) Dipeptidyl peptidase 4 as a therapeutic target in ischemia/reperfusion injury. Pharmacol Ther 136:267–282

    Article  CAS  PubMed  Google Scholar 

  24. Ou X, O’Leary HA, Broxmeyer HE (2013) Implications of DPP4 modification of proteins that regulate stem/progenitor and more mature cell types. Blood 122:161–169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Batra S, Kuo C, Rakusan K (1989) Spatial distribution of coronary capillaries: A-V segment staggering. Adv Exp Med Biol 248:241–247

    Article  CAS  PubMed  Google Scholar 

  26. Batra S, Konig MF, Cruz-Orives LM (1995) Unbiased estimation of capillary length from vertical slices. J Microscopy 178(Pt 2):152–159

    Article  Google Scholar 

  27. Dimitrova D, Tashkova S, Dikov A et al (2002) Enzyme activities of human milk cells. Comptes rendus de l’Academie Bulgare des Sciences, Tome, p 55

    Google Scholar 

  28. Lojda Z, Gossrau R, Schiebler TH (1979) Enzyme histochemistry.A laboratory manual. Springer, Berlin

    Book  Google Scholar 

  29. Van Noorden CJF, Fredericks WM (1992) Enzyme histochemistry. A laboratory manual of current methods. Oxford Science Publications. Oxford University Press – Royal Microscopy Society, Oxford

    Google Scholar 

  30. Horobin RW (1982) Histochemistry. Pag 135, Fig. 47. Fisher & Butterworths eds

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy

    Vittorio Bertone, Eleonora Tarantola & Isabel Freitas

Authors
  1. Vittorio Bertone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eleonora Tarantola
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Isabel Freitas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vittorio Bertone .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Bertone, V., Tarantola, E., Freitas, I. (2017). Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation