Abstract
Conventional microscopic techniques may be easily employed to detect and characterize cytotoxic T lymphocytes (CTL). Immunohistochemistry (IHC) may be effectively performed in CTL characterization to identify the location and distribution of target antigens in suspension cells or in tissues by staining with a specific antibody. The antibody may be conjugated to either a fluorescent or an enzymatic label, and the location of the label seen through a microscope approximates the position of the target antigen.
Here, immunohistochemistry and immunofluorescence for both paraffin-embedded and frozen tissues and for suspended cell are described. Moreover, specific recommendations for analysis of suspension of cells are provided.
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Acknowledgement
This work was funded by MERIT Program n. RBNE08BNL7 and by PRIN2008 Program n. 2008M9WSJX_002, both from the Italian Ministry of Education and University.
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Netti, G.S. (2014). Characterization of CTL by Microscopy. In: Ranieri, E. (eds) Cytotoxic T-Cells. Methods in Molecular Biology, vol 1186. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1158-5_8
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DOI: https://doi.org/10.1007/978-1-4939-1158-5_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1158-5
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