Abstract
The main difficulty for the cultivation and conservation of bromeliad species is the reduced number of propagules and slow growth of many of the species, resulting in a low propagation efficiency. Bromeliad plants are hardy and relatively easy to cultivate, with a high ornamental and ecological importance. Aiming at efficient micropropagation rates of V. hieroglyphica, a highly valued bromeliad, with very low propagation efficiency, a temporary immersion system was used and compared to semisolid and liquid static medium. Cultures obtained from in vitro germinated seeds were used as explants, maintaining their genetic diversity. Micropropagation with this simple temporary immersion system, composed of two autoclavable flasks, each with one opening for the attachment of 22 μm syringe filters, connected by a rubber stopper and an inner glass tube. In the bottom flask, an air valve is attached to the filter, which is subsequently connected to an aquarium pump and a timer and plugged to an outlet. This simple temporary immersion system showed improved micropropagation efficiency and is a method that can also be evaluated for other species.
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Acknowledgments
All authors acknowledge Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, SP, Brazil, for a doctoral scholarship to ANAP (2000/07987-5); CNPq, DF, Brazil, for a research fellowship to APM (304659/2005-3); and João Geraldo Brancalion, CENA/USP for graphics assistance.
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de Oliveira, C.R., Aranda-Peres, A.N., Soriano, L., Rodrigues, P.H.V., Martinelli, A.P. (2024). Increased Multiplication Rates of Vriesea hieroglyphica (Carriere) E. Morren Through a Temporary Immersion System. In: Ramírez-Mosqueda, M.A., Cruz-Cruz, C.A. (eds) Micropropagation Methods in Temporary Immersion Systems. Methods in Molecular Biology, vol 2759. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3654-1_12
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DOI: https://doi.org/10.1007/978-1-0716-3654-1_12
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