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Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

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Cardiac Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2573))

Abstract

Percutaneous antegrade coronary injection is among the least invasive cardiac selective gene delivery methods. However, the transduction efficiency of a simple bolus antegrade injection is quite low. In order to improve transduction efficiency in antegrade intracoronary delivery, several additional approaches have been proposed.

In this chapter, we will describe the important elements associated with intracoronary delivery methods and present protocols for three different catheter-based antegrade gene delivery techniques in a preclinical large animal model. This is the second edition of this chapter, and it includes modifications we have made over the past several years that further enhance transduction efficacy.

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Acknowledgments

This work was supported by NIH R01 HL139963 (K.I). We acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health for providing the gene vectors used in this study. S.A.M. was supported by National Institutes of Health T32 HL007824-23.

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Authors and Affiliations

  1. Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Spyros A. Mavropoulos, Kelly P. Yamada, Tomoki Sakata & Kiyotake Ishikawa

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  1. Spyros A. Mavropoulos
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  2. Kelly P. Yamada
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  3. Tomoki Sakata
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  4. Kiyotake Ishikawa
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Correspondence to Kiyotake Ishikawa .

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  1. Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Kiyotake Ishikawa

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Mavropoulos, S.A., Yamada, K.P., Sakata, T., Ishikawa, K. (2022). Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques. In: Ishikawa, K. (eds) Cardiac Gene Therapy. Methods in Molecular Biology, vol 2573. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2707-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2707-5_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2706-8

  • Online ISBN: 978-1-0716-2707-5

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