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Somatic Embryogenesis in Sugarcane (Saccharum spp.)

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Somatic Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2527))

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Abstract

Somatic embryogenesis is the process by which embryos are formed from a single or small group of somatic cells in response to specific stimuli. Somatic embryogenesis has been applied to achieve mass clonal propagation on an industrial scale and to increase the agronomic performance of species of economic interest, including sugarcane. The use of somatic embryogenesis in sugarcane stands out as a biotechnological tool with a high potential for application in the clonal propagation of disease-free elite varieties, as an essential part of genetic transformation protocols, and in the production of synthetic seeds. A better understanding of each phase of somatic embryogenesis can help to optimize the process to enhance yields and produce high-quality emblings. In this chapter, we describe a detailed protocol for somatic embryogenesis in sugarcane (Saccharum sp.) to be used in research projects for small-scale production. This protocol comprises all steps from explant preparation to the establishment of sugarcane emblings.

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References

  1. Ayodele BV, Alsaffar MA, Mustapa SI (2020) An overview of integration opportunities for sustainable bioethanol production from first-and second-generation sugar-based feedstocks. J Clean Prod 245:118857. https://doi.org/10.1016/j.jclepro.2019.118857

    Article  Google Scholar 

  2. Fehér A (2019) Callus, dedifferentiation, totipotency, somatic embryogenesis: what these terms mean in the era of molecular plant biology? Front Plant Sci 10:536. https://doi.org/10.3389/fpls.2019.00536

    Article  PubMed  PubMed Central  Google Scholar 

  3. Heringer AS, Santa-Catarina C, Silveira V (2018) Insights from proteomic studies into plant somatic embryogenesis. Proteomics 18(5–6):1700265. https://doi.org/10.1002/pmic.201700265

    Article  CAS  Google Scholar 

  4. Silveira V, Vita AM, Macedo AF, Dias MFR, Floh EIS, Santa-Catarina C (2013) Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane. Plant Cell Tissue Organ Cult 114(3):351–364. https://doi.org/10.1007/s11240-013-0330-2

    Article  CAS  Google Scholar 

  5. Passamani LZ, Reis RS, Vale EM, Sousa KR, Aragão VP, Santa-Catarina C, Silveira V (2020) Long-term culture with 2, 4-dichlorophenoxyacetic acid affects embryogenic competence in sugarcane callus via changes in starch, polyamine and protein profiles. Plant Cell Tissue Organ Cult 140(2):415–429. https://doi.org/10.1007/s11240-019-01737-w

    Article  CAS  Google Scholar 

  6. Reis RS, Vale EM, Sousa KR, Santa-Catarina C, Silveira V (2021) Pretreatment free of 2,4-dichlorophenoxyacetic acid improves the differentiation of sugarcane somatic embryos by affecting the hormonal balance and the accumulation of reserves. Plant Cell Tissue Organ Cult 145(1):101–115. https://doi.org/10.1007/s11240-020-01995-z

    Article  CAS  Google Scholar 

  7. Heringer AS, Barroso T, Macedo AF, Santa-Catarina C, Souza GHMF, Floh EIS, de Souza-Filho GA, Silveira V (2015) Label-free quantitative proteomics of embryogenic and non-embryogenic callus during sugarcane somatic embryogenesis. PLoS One 10(6):e0127803. https://doi.org/10.1371/journal.pone.0127803

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Heringer AS, Reis RS, Passamani LZ, de Souza-Filho GA, Santa-Catarina C, Silveira V (2017) Comparative proteomics analysis of the effect of combined red and blue lights on sugarcane somatic embryogenesis. Acta Physiol Plant 39(2):52. https://doi.org/10.1007/s11738-017-2349-1

    Article  CAS  Google Scholar 

  9. Reis RS, Vale EM, Heringer AS, Santa-Catarina C, Silveira V (2016) Putrescine induces somatic embryo development and proteomic changes in embryogenic callus of sugarcane. J Proteome 130:170–179. https://doi.org/10.1016/j.jprot.2015.09.029

    Article  CAS  Google Scholar 

  10. Passamani LZ, Bertolazi AA, Ramos AC, Santa-Catarina C, Thelen JJ, Silveira V (2018) Embryogenic competence acquisition in sugarcane callus is associated with differential H+-pump abundance and activity. J Proteome Res 17(8):2767–2779. https://doi.org/10.1021/acs.jproteome.8b00213

    Article  CAS  PubMed  Google Scholar 

  11. Almeida FA, Passamani LZ, Santa-Catarina C, Mooney BP, Thelen JJ, Silveira V (2020) Label-free quantitative phosphoproteomics reveals signaling dynamics involved in embryogenic competence acquisition in sugarcane. J Proteome Res 19(10):4145–4157. https://doi.org/10.1021/acs.jproteome.0c00652

    Article  CAS  PubMed  Google Scholar 

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil

    Felipe Astolpho Almeida & Vanildo Silveira

  2. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil

    Felipe Astolpho Almeida & Vanildo Silveira

  3. Laboratório de Biologia Celular e Tecidual, CBB-UENF, Campos dos Goytacazes, RJ, Brazil

    Claudete Santa-Catarina

Authors
  1. Felipe Astolpho Almeida
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  2. Claudete Santa-Catarina
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  3. Vanildo Silveira
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Corresponding author

Correspondence to Vanildo Silveira .

Editor information

Editors and Affiliations

  1. Laboratorio de Micropropagación Vegetal, Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Almeida, F.A., Santa-Catarina, C., Silveira, V. (2022). Somatic Embryogenesis in Sugarcane (Saccharum spp.). In: Ramírez-Mosqueda, M.A. (eds) Somatic Embryogenesis. Methods in Molecular Biology, vol 2527. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2485-2_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2485-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2484-5

  • Online ISBN: 978-1-0716-2485-2

  • eBook Packages: Springer Protocols

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