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Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy

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Photodynamic Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2451))

Abstract

Classic preclinical investigations on the mechanisms and effects of photodynamic therapy (PDT) are typically performed in two-dimensional cell cultures that have some, albeit limited, relevance to cancer biology. Bioengineered three-dimensional (3D) culture models of cancer are gaining traction in translational oncology as microtumors recapitulate the tumor architectures and cellular heterogeneity more faithfully than conventional 2D cultures. These 3D models bridge a gap between highly relevant but low-throughput in vivo animal models and high-throughput two-dimensional cultures with low clinical relevance, and thus hold promise as preclinical testing platforms in PDT research. Here, we discuss the potential applications of organotypic cancer models for PDT research and provide two well-established methodologies for generating 3D cultures of cancer: a liquid-suspended spheroid model and an adherent microtumor culture model grown on extracellular matrix scaffolds. Particular emphasis is given to harvesting the cultures for the purpose of immunoblotting and flow cytometry.

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Author information

Authors and Affiliations

  1. Institute for Advanced Biosciences, INSERM U1209, CNRS UMR 5309, Université Grenoble Alpes, Grenoble, France

    Mans Broekgaarden & Jean-Luc Coll

Authors
  1. Mans Broekgaarden
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  2. Jean-Luc Coll
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Corresponding author

Correspondence to Mans Broekgaarden .

Editor information

Editors and Affiliations

  1. Institute for Advanced Biosciences, Université de Grenoble Alpes, Grenoble, France

    Mans Broekgaarden

  2. van ‘t Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands

    Hong Zhang

  3. British Columbia Cancer Research Centre, Vancouver, BC, Canada

    Mladen Korbelik

  4. Laser Research Centre, University of Johannesburg, Doornfontein, South Africa

    Michael R. Hamblin

  5. Jiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, Jiaxing University College of Medicine, Jiaxing, Zhejiang, China

    Michal Heger

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Broekgaarden, M., Coll, JL. (2022). Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy. In: Broekgaarden, M., Zhang, H., Korbelik, M., Hamblin, M.R., Heger, M. (eds) Photodynamic Therapy. Methods in Molecular Biology, vol 2451. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2099-1_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2099-1_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2098-4

  • Online ISBN: 978-1-0716-2099-1

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