Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)

  • Protocol
  • First Online:
Membrane Lipids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2402))

  • 901 Accesses

Abstract

Model lipid bilayers tethered to a gold substrate with molecular tethers are constructed. The conductance versus temperature dependence curve is then obtained. Here, a method to measure the activation energy for translocation of an ion through existing transmembrane pores in a sparsely tethered bilayer lipid membranes is presented.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
EUR 44.95
Price includes VAT (France)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
EUR 106.99
Price includes VAT (France)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
EUR 137.14
Price includes VAT (France)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
EUR 210.99
Price includes VAT (France)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Parsegian A (1969) Energy of an ion crossing a low dielectric membrane: solutions to four relevant electrostatic problems. Nature 221(5183):844–846

    Article  CAS  Google Scholar 

  2. Coster H, Laver D (1984) The conductance of lecithin bilayers: the dependence on temperature. Chem Phys Lipids 34:227–238

    Article  Google Scholar 

  3. Hoiles W, Krishnamurthy V, Cranfield CG et al (2014) An engineered membrane to measure electroporation: effect of tethers and bioelectronic interface. Biophys J 107(6):1339–1351

    Article  CAS  Google Scholar 

  4. Cranfield C, Carne S, Martinac B et al (2015) The assembly and use of tethered bilayer lipid membranes (tBLMs). In: Methods in membrane lipids. Springer, New York, pp 45–53

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hadeel Alobeedallah .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Alobeedallah, H., Cornell, B.A., Coster, H. (2022). Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs). In: Cranfield, C.G. (eds) Membrane Lipids. Methods in Molecular Biology, vol 2402. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1843-1_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1843-1_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1842-4

  • Online ISBN: 978-1-0716-1843-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Navigation