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Production of Haploid and Doubled Haploid Lines in Nut Crops: Persian Walnut, Almond, and Hazelnut

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2289))

Abstract

This chapter deals with induction of haploidy via parthenogenesis in Persian walnut and via microspore embryogenesis in almond and hazelnut. Haploid induction through in situ parthenogenesis using pollination with irradiated pollen to stimulate the embryogenic development of the egg cell, followed by in vitro culture of the immature haploid embryos. Microspore embryogenesis allows the induction of immature pollen grains (microspores), to move away from the normal gametophytic developmental route in the direction of the sporophytic one, yielding homozygous organisms (embryos in this case). Unlike other fruit crops (such as Citrus), regeneration of entire plants has not yet been obtained in our studied nut crops; however, it gives the methodology should be used to continue the roadmap.

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Acknowledgments

The Authors thank all people working on gametic embryogenesis of nut crops. Iran National Science Foundation (INSF) and Center of Excellence for Walnut Improvement and Technology of Iran are acknowledged for their supports. This review has been partially supported by project “Breeding stone fruit species assisted by molecular tools” from the Seneca Foundation of the Region of Murcia in Spain (19879/GERM/15).

Author information

Authors and Affiliations

  1. Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran

    Kourosh Vahdati

  2. Department of Horticulture, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

    Mohammad Sadat-Hosseini

  3. Department of Plant Breeding, CEBAS-CSIC, Murcia, Spain

    Pedro Martínez-Gómez

  4. Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Sudi di Palermo, Palermo, Italy

    Maria Antonietta Germanà

Authors
  1. Kourosh Vahdati
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  2. Mohammad Sadat-Hosseini
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  3. Pedro Martínez-Gómez
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  4. Maria Antonietta Germanà
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Corresponding author

Correspondence to Kourosh Vahdati .

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Editors and Affiliations

  1. Cell Biology Group — COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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Vahdati, K., Sadat-Hosseini, M., Martínez-Gómez, P., Germanà, M.A. (2021). Production of Haploid and Doubled Haploid Lines in Nut Crops: Persian Walnut, Almond, and Hazelnut. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2289. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1331-3_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1331-3_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1330-6

  • Online ISBN: 978-1-0716-1331-3

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