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Rodent Models of Congenital Cytomegalovirus Infection

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Human Cytomegaloviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2244))

Abstract

Human cytomegalovirus (HCMV) is a leading viral cause of congenital infections in the central nervous system (CNS) and may result in severe long-term sequelae. High rates of sequelae following congenital HCMV infection and insufficient antiviral therapy in the perinatal period makes the development of an HCMV-specific vaccine a high priority of modern medicine. Due to the species specificity of HCMV, animal models are frequently used to study CMV pathogenesis. Studies of murine cytomegalovirus (MCMV) infections of adult mice have played a significant role as a model of CMV biology and pathogenesis, while MCMV infection of newborn mice has been successfully used as a model of perinatal CMV infection. Newborn mice infected with MCMV have high levels of viremia during which the virus establishes a productive infection in most organs, coupled with a robust inflammatory response. Productive infection in the brain parenchyma during early postnatal period leads to an extensive nonnecrotizing multifocal widespread encephalitis characterized by infiltration of components of both innate and adaptive immunity. As a result, impairment in postnatal development of mouse cerebellum leads to long-term motor and sensor disabilities. This chapter summarizes current findings of rodent models of perinatal CMV infection and describes methods for analysis of perinatal MCMV infection in newborn mice.

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Acknowledgments

This work has been supported by the Croatian Science Foundation under the project IP-2016-06-5980 (VJL), University of Rijeka under the project uniri-biomed-18-234 (BL) and the grant KK.01.1.1.01.0006, awarded to the Scientific Centre of Excellence for Virus Immunology and Vaccines and cofinanced by the European Regional Development Fund (SJ).

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Authors and Affiliations

  1. Department for Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia

    Berislav Lisnić, Jelena Tomac, Djurdjica Cekinović, Stipan Jonjić & Vanda Juranić Lisnić

  2. Department of Infectious Diseases, Faculty of Medicine, University of Rijeka, Rijeka, Croatia

    Djurdjica Cekinović

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  1. Berislav Lisnić
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  2. Jelena Tomac
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  3. Djurdjica Cekinović
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  4. Stipan Jonjić
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  5. Vanda Juranić Lisnić
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Correspondence to Stipan Jonjić .

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  1. Department of Microbiology and Immunology, Louisiana State University Health Sciences Center—Shreveport, Shreveport, LA, USA

    Andrew D. Yurochko

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Lisnić, B., Tomac, J., Cekinović, D., Jonjić, S., Juranić Lisnić, V. (2021). Rodent Models of Congenital Cytomegalovirus Infection. In: Yurochko, A.D. (eds) Human Cytomegaloviruses. Methods in Molecular Biology, vol 2244. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1111-1_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1111-1_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1110-4

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