Abstract
Type-I hypersensitivity is commonly characterized by increased levels of antigen-specific immunoglobulin (Ig) E. Therefore, it is important for clinical and research investigators to reliably measure serum levels of IgE in allergic patients and animal models. While current ELISA-based methods are simple and commonly performed for the detection of allergen-specific IgE using serum or plasma, they may produce misleading results. This is in part due to decreased sensitivity for IgE in the presence of other Ig isotypes in the same sample, such as IgG, that are typically more abundant than IgE. When assessment of multiple Ig isotypes is necessary, performing optimized assays for individual isotypes requires high sample volumes. Here, we describe an approach to increase the sensitivity for IgE detection while conserving the sample volume needed. This method not only improves the accuracy of serum IgE measurements but also allows simultaneous analysis of other allergen-specific immunoglobulins.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Galli SJ, Tsai M (2012) IgE and mast cells in allergic disease. Nat Med 18(5):693–704. https://doi.org/10.1038/nm.2755
Schroeder HW Jr, Cavacini L (2010) Structure and function of immunoglobulins. J Allergy Clin Immunol 125(2 Suppl 2):S41–S52. https://doi.org/10.1016/j.jaci.2009.09.046
Finkelman FD, Khodoun MV, Strait R (2016) Human IgE-independent systemic anaphylaxis. J All Clin Immunol 137(6):1674–1680. https://doi.org/10.1016/j.jaci.2016.02.015
Gocki J, Zbigniew B (2016) Role of immunoglobulin G antibodies in diagnosis of food allergy. Postepy Dermatol Alergol 33(4):253–256. https://doi.org/10.5114/ada.2016.61600
Yel L (2010) Selective IgA deficiency. J Clin Immunol 30(1):10–16. https://doi.org/10.1007/s10875-009-9357-x
Lehrer SB, Reish R, Fernandes J et al (2004) Enhancement of murine IgE antibody detection by IgG removal. J Immunol Methods 284(1–2):1–6. https://doi.org/10.1016/j.jim.2003.08.017
Choe W, Durgannavar TA, Chung SJ (2016) Fc-Binding Ligands of Immunoglobulin G: an overview of high affinity proteins and peptides. Materials (Basel) 9(12):994. https://doi.org/10.3390/ma9120994
Smith NA, Germundson DL, Combs CK et al (2019) Astrogliosis associated with behavioral abnormality in a non-anaphylactic mouse model of cow’s milk allergy. Front Cell Neurosci 13:320. https://doi.org/10.3389/fncel.2019.00320
Germundson DL, Smith NA, Vendsel LP et al (2018) Oral sensitization to whey proteins induces age- and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food allergy: a potential role of mast cells. J Neuroinflammation 15:120. https://doi.org/10.1186/s12974-018-1146-0
Acknowledgments
This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under grant numbers P20GM103442 and 5P20GM113123.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Germundson, D.L., Nagamoto-Combs, K. (2021). Isotype-Specific Detection of Serum Immunoglobulins Against Allergens. In: Nagamoto-Combs, K. (eds) Animal Models of Allergic Disease. Methods in Molecular Biology, vol 2223. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1001-5_12
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1001-5_12
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1000-8
Online ISBN: 978-1-0716-1001-5
eBook Packages: Springer Protocols