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Molecular Cytogenetics (Fluorescence In Situ Hybridization - FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2222))

Abstract

Fluorochrome banding (chromomycin, Hoechst, and DAPI) and fluorescence in situ hybridization (FISH) are excellent molecular cytogenetic tools providing various possibilities in the study of chromosomal evolution and genome organization. The constitutive heterochromatin and rRNA genes are the most widely used FISH markers. The rDNA is organized into two distinct gene families (18S–5.8S–26S and 5S) whose number and location vary within the complex of closely related species. Therefore, they are widely used as chromosomal landmarks to provide valuable evidence concerning genome evolution at chromosomal levels.

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Author information

Authors and Affiliations

  1. University Paris-Saclay, CNRS, AgroParisTech, Ecologie Systématique Evolution, Orsay, France

    Sonja Siljak-Yakovlev & Odile Robin

  2. Faculty of Forestry, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

    Fatima Pustahija

  3. Faculty of Science, Department of Molecular Biology, University of Zagreb, Zagreb, Croatia

    Vedrana Vičić-Bočkor

Authors
  1. Sonja Siljak-Yakovlev
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  2. Fatima Pustahija
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  3. Vedrana Vičić-Bočkor
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  4. Odile Robin
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Corresponding author

Correspondence to Sonja Siljak-Yakovlev .

Editor information

Editors and Affiliations

  1. UMR PVBMT, Universite de la Reunion, St Pierre, Réunion, France

    Pascale Besse

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Siljak-Yakovlev, S., Pustahija, F., Vičić-Bočkor, V., Robin, O. (2021). Molecular Cytogenetics (Fluorescence In Situ Hybridization - FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 2222. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0997-2_18

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  • DOI: https://doi.org/10.1007/978-1-0716-0997-2_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0996-5

  • Online ISBN: 978-1-0716-0997-2

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