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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Reversible suspension culture of human vascular smooth muscle cells using the functional biopolymer FP003
Human vascular smooth muscle cells (SMCs) are adherent cells, and they cannot survive without scaffolds in suspension culture. Here, we aimed to...
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Suspension culture of stem cells established of Calendula officinalis L.
Plant stem cell cultures have so far been established in only a few plant species using cambial meristematic cells. The presence of stem cells or...
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Inoculation and Passaging of Adherent and Suspension Cells
Cell culture is an essential and necessary technique used in cell culture, unbiased with the growth of various organisms by introducing of various...
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Scalable Generation of 3D Pancreatic Islet Organoids from Human Pluripotent Stem Cells in Suspension Bioreactors
We describe a scalable method for the robust generation of 3D pancreatic islet-like organoids from human pluripotent stem cells using suspension...
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Suspension
The suspension is the major part of the chassis. It determines the position of the tire and thus becomes the most important assembly of a vehicle,...
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Hydrogen sulfide promoted cell differentiation, antioxidant ability, and flavonoids accumulation in Ginkgo biloba L. suspension cells
Hydrogen sulfide (H 2 S) as the third gasotransmitter could regulate many physiological processes such as growth and stress responses in plants....
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Fusion gene 4CL-CCR promotes lignification in tobacco suspension cells
Key messageThe fusion gene 4CL-CCR promotes lignification and activates lignin-related MYB expression in tobacco but inhibits auxin-related gene...
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Advanced Systems and Bioreactors for Large-Scale Secondary Metabolite Production in Medicinal Plants Using Suspension Cultured Cells
In recent years, there has been extensive research on large-scale plant secondary metabolite production systems and bioreactors for various...
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Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quality analysis of the regenerated tubers
We established optimal conditions for the mass production of microtubers from suspension cultures of Pinellia ternata cells including the relative...
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Protocol development to further differentiate and transition stem cell-derived pancreatic progenitors from a monolayer into endocrine cells in suspension culture
The generation of functional β-cells from human pluripotent stem cells (hPSCs) for cell replacement therapy and disease modeling of diabetes is being...
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Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
Most pharmaceutical biotechnology companies use stirred-tank bioreactors (STR) for recombinant protein manufacturing. These bioreactors are used at a...
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Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression
Suspension cells derived from human embryonic kidney cells (HEK 293) are attractive cell lines for retroviral vectorRetroviral vectors production in...
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Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation
Inducing the differentiation of specific cell type(s) synchronously and on-demand is a great experimental system to understand the sequential...
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Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
Immunoglobulin A (IgA) has been showing potential as a new therapeutic antibody. However, recombinant IgA suffers from low yield. Supplementation of...
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In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells
Transport vesicles mediate protein traffic between endomembrane organelles in a highly selective and efficient manner. In vitro reconstitution...
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Biotechnology of Cultivation of Rhaponticum Carthamoides (willd.) Suspension Cells: A Prospective Source of Antitumor Substances
AbstractTo preserve the species diversity of Rhaponticum carthamoides , a Red Book plant that is a promising source of antitumor metabolites, it is...
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NaCl-induced flavonoid biosynthesis and oxidative stress responses in suspension cells of Haplophyllum virgatum var. virgatum
In addition to causing oxidative stress, NaCl can act as an inexpensive elicitor to trigger the biosynthesis of secondary metabolites like...
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A feasibility study of different commercially available serum-free mediums to enhance lentivirus and adeno-associated virus production in HEK 293 suspension cells
Lentivirus and adeno-associated viruses are invaluable tools for biotechnology applications due to their genetic material delivery abilities both...
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Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
Microfluidic cultivation, with its high level of environmental control and spatio-temporal resolution of cellular behavior, is a well-established...
