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Optimizing yeast for high-level production of kaempferol and quercetin
BackgroundTwo important flavonoids, kaempferol and quercetin possess remarkably potent biological impacts on human health. However, their structural...
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Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors
BackgroundThe fungal natural products illudin S and M have been investigated as precursors for the development of semisynthetic anticancer agents...
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Metabolic engineering of yeast for the production of carbohydrate-derived foods and chemicals from C1–3 molecules
The increase in population-related and environmental issues has emphasized the need for more efficient and sustainable production strategies for...
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Physiological and stoichiometric characterization of ethanol-based chain elongation in the absence of short-chain carboxylic acids
Hexanoate is a valuable chemical that can be produced by microorganisms that convert short-chain- to medium-chain carboxylic acids through a process...
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Investigations on riboflavin production by wild-type yeast strain for supplementation of organic feed
Supplementation of vitamin B2 (riboflavin) in poultry feeding is essential. For economic and environmental reasons, riboflavin is now produced...
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Itaconate Production from Crude Substrates with U. maydis: Scale-up of an Industrially Relevant Bioprocess
BackgroundIndustrial by-products accrue in most agricultural or food-related production processes, but additional value chains have already been...
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Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Human induced pluripotent stem cells (hiPSCs) can be expanded at limitless scale in vitro and give rise to various organotypic cells, cardiomyocytes...
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Metabolic reconfiguration enables synthetic reductive metabolism in yeast
Cell proliferation requires the integration of catabolic processes to provide energy, redox power and biosynthetic precursors. Here we show how the...
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High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum
BackgroundExtremolytes enable microbes to withstand even the most extreme conditions in nature. Due to their unique protective properties, the small...
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High-throughput process development from gene cloning to protein production
In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In...
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Matrix-free human pluripotent stem cell manufacturing by seed train approach and intermediate cryopreservation
BackgroundHuman pluripotent stem cells (hPSCs) have an enormous therapeutic potential, but large quantities of cells will need to be supplied by...
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Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
BackgroundThe abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial...
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Metabolic control analysis enabled the improvement of the L-cysteine production process with Escherichia coli
AbstractL-cysteine is an amino acid with relevance to the pharmaceutical, food, feed, and cosmetic industry. The environmental and societal impact of...
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From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris
BackgroundExpansion of the genetic code is a frequently employed approach for the modification of recombinant protein properties. It involves...
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First time β-farnesene production by the versatile bacterium Cupriavidus necator
BackgroundTerpenes are remarkably diverse natural structures, which can be formed via two different pathways leading to two common intermediates....
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High shear resistance of insect cells: the basis for substantial improvements in cell culture process design
Multicellular organisms cultivated in continuous stirred tank reactors (CSTRs) are more sensitive to environmental conditions in the suspension...
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Production of a newly discovered PHA family member with an isobutyrate-fed enrichment culture
AbstractUsing microbial enrichment cultures for the production of waste-derived polyhydroxyalkanoates (PHAs) is a promising technology to recover...
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Control of parallelized bioreactors II: probabilistic quantification of carboxylic acid reductase activity for bioprocess optimization
Autonomously operated parallelized mL-scale bioreactors are considered the key to reduce bioprocess development cost and time. However, their...
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Parallel Reactor Systems for Bioprocess Development
Controlled parallel bioreactor systems allow fed-batch operation at early stages of process development. The characteristics of shaken bioreactors...
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A production platform for disulfide-bonded peptides in the periplasm of Escherichia coli
BackgroundRecombinant peptide production in Escherichia coli provides a sustainable alternative to environmentally harmful and size-limited chemical...
