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Detecting gene breakpoints in noisy genome sequences using position-annotated colored de-Bruijn graphs
BackgroundIdentifying the locations of gene breakpoints between species of different taxonomic groups can provide useful insights into the underlying...
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PCR-Based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis
The ability to enzymatically assemble DNA oligonucleotides into longer DNA duplexes in a process known as gene synthesis has wide-ranging...
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Reporter gene assays and chromatin-level assays define substantially non-overlap** sets of enhancer sequences
BackgroundTranscriptional enhancers are essential for gene regulation, but how these regulatory elements are best defined remains a significant...
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Population genetic structure of Randall’s threadfin bream Nemipterus randalli in Indian waters based on mitochondrial and nuclear gene sequences
Nemipterus randalli , commonly known as Randall’s threadfin bream, is a commercially important marine finfish. Understanding its genetic structure is...
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A two-sequence motif-based method for the inventory of gene families in fragmented and poorly annotated genome sequences
Databases of genome sequences are growing exponentially, but, in some cases, assembly is incomplete and genes are poorly annotated. For evolutionary...
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Higher evolutionary dynamics of gene copy number for Drosophila glue genes located near short repeat sequences
BackgroundDuring evolution, genes can experience duplications, losses, inversions and gene conversions. Why certain genes are more dynamic than...
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Genetic diversity and relationship among Indonesian local sheep breeds on Java Island based on mitochondrial cytochrome b gene sequences
BackgroundThe cytochrome b (Cyt b) gene is one of the most studied mitochondrial DNA (mtDNA) genes to determine sheep’s genetic profile. This study...
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Metagenomic Gene Prediction Using Bidirectional LSTM
Genomics and microbial research have been revolutionized by the beginning of low-cost, high-throughput sequencing technologies. Which, in turn,...
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Prevalence of Deltaproteobacterial sequences in nifH gene pools associated with the rhizosphere of native switchgrass from Tall Grass Prairie (Oklahoma, USA)
The potential nitrogen-fixing bacterial diversity in the rhizospheric soil of the native switchgrass ( Panicum virgatum L.) from Tall Grass Prairies...
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dCas9-based gene editing for cleavage-free genomic knock-in of long sequences
Gene editing is a powerful tool for genome and cell engineering. Exemplified by CRISPR–Cas, gene editing could cause DNA damage and trigger DNA...
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Nanodelivery in Gene Therapy
Gene therapy stands as a revolutionary approach in medicine, aiming to rectify genetic abnormalities by introducing therapeutic genes or regulating...
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Gene Expression in Eukaryotes
Eukaryotes are multicellular organisms and thus have a complex process of gene regulation. In eukaryotes, the gene sequences are not continuous to...
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An insight into misidentification of the small-subunit ribosomal RNA (18S rRNA) gene sequences of Theileria spp. as Theileria annulata
BackgroundThere had been isolated reports of the presence of novel Theileria annulata genotypes based on the 18S rRNA gene sequence data from India,...
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Pango lineage designation and assignment using SARS-CoV-2 spike gene nucleotide sequences
BackgroundMore than 2 million SARS-CoV-2 genome sequences have been generated and shared since the start of the COVID-19 pandemic and constitute a...
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Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences
Western Subarctic Gyre (WSG), which possesses distinctive differences in oceanographic and biogeochemical processes, is situated in the northwest...
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Clustering Sequences into OTUs
This chapter describes and illustrates taxonomic classification of the representative sequences and clustering of OTUs. First it introduces some...
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Gene Expression
During the normal functioning of living organisms, proteins are expressed according to well-regulated mechanisms to meet specific needs, this process...
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Virus-Induced Heritable Gene Editing in Plants
Gene editing using clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nuclease is an excellent tool for...
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Intronic small nucleolar RNAs regulate host gene splicing through base pairing with their adjacent intronic sequences
BackgroundSmall nucleolar RNAs (snoRNAs) are abundant noncoding RNAs best known for their involvement in ribosomal RNA maturation. In mammals, most...
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Genetic analyses of rabies virus glycoprotein and nucleoprotein gene sequences reveal the emergence of multiple lineages in animals in Arkhangai province, a central region of Mongolia
Genetic characterizations of rabies viruses circulating in carnivore and non-carnivore animals were investigated for the first time in Arkhangai...
