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Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites
Here we present an approach that combines a clustered regularly interspaced short palindromic repeats (CRISPR) system that simultaneously targets...
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Genome-Wide Expression Analysis of Genes Involved in Somatic Embryogenesis
Genome-wide expression analysis is an important tool for identifying and analysing genes involved in various biological processes, including cell...
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Massively parallel phenoty** of coding variants in cancer with Perturb-seq
Genome sequencing studies have identified millions of somatic variants in cancer, but it remains challenging to predict the phenotypic impact of...
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Forensic nanopore sequencing of STRs and SNPs using Verogen’s ForenSeq DNA Signature Prep Kit and MinION
The MinION nanopore sequencing device (Oxford Nanopore Technologies, Oxford, UK) is the smallest commercially available sequencer and can be used...
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Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This...
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Next-Generation Sequencing in Medicinal Plants: Recent Progress, Opportunities, and Challenges
Medicinal plants have a significant role in human health care since the development of medicine. Plants that yield phytopharmaceuticals have produced...
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Single duplex DNA sequencing with CODEC detects mutations with high sensitivity
Detecting mutations from single DNA molecules is crucial in many fields but challenging. Next-generation sequencing (NGS) affords tremendous...
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Human early-onset dementia caused by DAP12 deficiency reveals a unique signature of dysregulated microglia
The TREM2–DAP12 receptor complex sustains microglia functions. Heterozygous hypofunctional TREM2 variants impair microglia, accelerating late-onset...
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Massively parallel single-cell mitochondrial DNA genoty** and chromatin profiling
Natural mitochondrial DNA (mtDNA) mutations enable the inference of clonal relationships among cells. mtDNA can be profiled along with measures of...
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Role of Next-Generation Sequencing (NGS) in Understanding the Microbial Diversity
Microbes constitute a major part of earth’s biological diversity and act as a key player by playing an important role in our day-to-day life....
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The Role of Transcriptional Profiling in Neurobiology and Treatment of Major Depression: A Translational Perspective on RNA Sequencing Platform
High-throughput transcriptomic profiling is a powerful experimental strategy to understand complex neurobiological mechanisms associated with many...
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RNA Sequencing (RNA-seq)
RNA sequencing (or popularly known as RNA-seq) or whole transcriptome shortgun sequencing (WTSS) is a useful next-generation sequencing (NGS)...
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Single-cell RNA sequencing for the study of kidney disease
The kidney is an important organ for maintaining normal metabolism and stabilising the internal environment, in which, the heterogeneity of cell...
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Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation
The forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in...
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Commonalities and differences in the mutational signature and somatic driver mutation landscape across solid and hollow viscus organs
Advances in sequencing have revealed a highly variegated landscape of mutational signatures and somatic driver mutations in a range of normal...
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A shared disease-associated oligodendrocyte signature among multiple CNS pathologies
Alzheimer’s disease (AD) is a complex neurodegenerative disease, perturbing neuronal and non-neuronal cell populations. In this study, using...
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A transcriptional metastatic signature predicts survival in clear cell renal cell carcinoma
Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer in adults. When ccRCC is localized to the kidney, surgical resection...
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Development and validation of a novel 133-plex forensic STR panel (52 STRs and 81 Y-STRs) using single-end 400 bp massive parallel sequencing
Short tandem repeats (STRs) are the preferred genetic markers in forensic DNA analysis, routinely measured by capillary electrophoresis (CE) method...
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Mitochondrial point heteroplasmy: insights from deep-sequencing of human replicate samples
BackgroundHuman mitochondrial heteroplasmy is an extensively investigated phenomenon in the context of medical diagnostics, forensic identification...
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Advances in single-cell RNA sequencing and its applications in cancer research
Cancers are a group of heterogeneous diseases characterized by the acquisition of functional capabilities during the transition from a normal to a...
