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Nano-delivery platforms for bacterial gene transformation: suitability and challenges
Nano-scale particles (NPs) have gained increased interest as non-viral vectors for nucleic acid delivery due to their ability to penetrate through...
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Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been used for the insertion of...
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In vitro DNA plasmid condensation and transfection through pH-responsive nanohydrogel
Nanohydrogels (NHs) with the benefits of both nanomaterials and hydrogels unlock novel opportunities and applications in biomedicine. Nowadays,...
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Aerosolizable Plasmid DNA Dry Powders Engineered by Thin-film Freezing
PurposeThis study was designed to test the feasibility of using thin-film freezing (TFF) to prepare aerosolizable dry powders of plasmid DNA (pDNA)...
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Towards Development of the 4C-Based Method Detecting Interactions of Plasmid DNA with Host Genome
AbstractChromosome conformation capture techniques have revolutionized our understanding of chromatin architecture and dynamics at the genome-wide...
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Nutrient and Drug Delivery Systems
This chapter deliberates on the utilisation of nanochitosan as an efficient delivery system for nutrients and drugs in fish and other kinds of...
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Effect of ursodiol on alginate/PLL nanoparticles with non-ionic surfactant for gene delivery
Hearing loss is a widespread condition, affecting people from a range of demographics. Gene therapy is an emerging method for the amelioration of...
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Use of Optical In Vivo Imaging to Monitor and Optimize Delivery of Novel Plasmid-Launched Live-Attenuated Vaccines
Plasmid-launched live-attenuated vaccines (PLLAV) are a modality of next-generation vaccines with the promise to combine the benefits of both (1) the...
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Investigation of the potential therapeutic effect of cationic lipoplex mediated fibroblast growth factor-2 encoding plasmid DNA delivery on wound healing
BackgroundDevelo** an alternative and efficient therapy for wound healing has been an important research topic for pharmaceutical sciences. A...
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Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems
BackgroundWhilst traditional strategies to increase transfection efficiency of non-viral systems aimed at modifying the vector or the...
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A self-transmissible plasmid from a hyperthermophile that facilitates genetic modification of diverse Archaea
Conjugative plasmids are self-transmissible mobile genetic elements that transfer DNA between host cells via type IV secretion systems (T4SS). While...
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VHEE FLASH sparing effect measured at CLEAR, CERN with DNA damage of pBR322 plasmid as a biological endpoint
Ultra-high dose rate (UHDR) irradiation has been shown to have a sparing effect on healthy tissue, an effect known as ‘FLASH’. This effect has been...
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Conjugative type IV secretion systems enable bacterial antagonism that operates independently of plasmid transfer
Bacterial cooperation and antagonism mediated by secretion systems are among the ways in which bacteria interact with one another. Here we report the...
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Materials for Gene Delivery Systems
Gene therapy has garnered a lot of interest in the recent past for the treatment of various life-threatening genetic diseases. Gene therapy involves...
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ZIF-8 Nanoparticle: A Valuable Tool for Improving Gene Delivery in Sperm-Mediated Gene Transfer
Metal–organic frameworks (MOFs) are porous materials with unique characteristics that make them well-suited for drug delivery and gene therapy...
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Rambutan-like silica nanoparticles at tailored particle sizes for plasmid DNA delivery
AbstractSilica nanoparticles (SNPs) have attracted widespread attention as biocompatible and efficient nanocarriers for gene delivery. The...
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Single molecule delivery into living cells
Controlled manipulation of cultured cells by delivery of exogenous macromolecules is a cornerstone of experimental biology. Here we describe a...
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Gene Delivery Using Polymer Therapeutics
The use of polymers as synthetic non-viral carriers for introducing nucleic acids into cells appears very appealing. Polymers can be generated in...
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Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains
BackgroundOwing to CRISPR-Cas9 and derivative technologies, genetic studies on microorganisms have dramatically increased. However, the CRISPR-Cas9...
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Complete sequence verification of plasmid DNA using the Oxford Nanopore Technologies’ MinION device
BackgroundSequence verification is essential for plasmids used as critical reagents or therapeutic products. Typically, high-quality plasmid sequence...
