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Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag
Cas9 can cleave DNA in both blunt and staggered configurations, resulting in distinct editing outcomes, but what dictates the type of Cas9 incisions...
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The DNA Double-Strand Break Repair in Glioma: Molecular Players and Therapeutic Strategies
Gliomas are the most frequent type of tumor in the central nervous system, which exhibit properties that make their treatment difficult, such as...
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Prolonged Particulate Hexavalent Chromium Exposure Induces DNA Double-Strand Breaks and Inhibits Homologous Recombination Repair in Primary Rodent Lung Cells
Hexavalent chromium [Cr(VI)] is a known lung carcinogen and a driving mechanism in human lung cells for Cr(VI)-induced lung cancer is chromosome...
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Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaks
FAAP20 is a Fanconi anemia (FA) protein that associates with the FA core complex to promote FANCD2/FANCI monoubiquitination and activate the damage...
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Double strand DNA breaks in sperm: the bad guy in the crowd
PurposeThe main objective of this opinion paper was to bring to light and enhance our understanding of the amount of double-strand DNA breaks in...
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Failure of DNA double-strand break repair by tau mediates Alzheimer’s disease pathology in vitro
DNA double-strand break (DSB) is the most severe form of DNA damage and accumulates with age, in which cytoskeletal proteins are polymerized to...
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Helicase Q promotes homology-driven DNA double-strand break repair and prevents tandem duplications
DNA double-strand breaks are a major threat to cellular survival and genetic integrity. In addition to high fidelity repair, three intrinsically...
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MRNIP condensates promote DNA double-strand break sensing and end resection
The rapid recognition of DNA double-strand breaks (DSBs) by the MRE11/RAD50/NBS1 (MRN) complex is critical for the initiation of DNA damage response...
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ATM inhibition exploits checkpoint defects and ATM-dependent double strand break repair in TP53-mutant glioblastoma
Determining the balance between DNA double strand break repair (DSBR) pathways is essential for understanding treatment response in cancer. We report...
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Liquid-liquid phase separation in DNA double-strand breaks repair
DNA double-strand breaks (DSBs) are the fatal type of DNA damage mostly induced by exposure genome to ionizing radiation or genotoxic chemicals. DSBs...
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Inhibition of key DNA double strand break repair protein kinases enhances radiosensitivity of head and neck cancer cells to X-ray and proton irradiation
Ionising radiation (IR) is widely used in cancer treatment, including for head and neck squamous cell carcinoma (HNSCC), where it induces significant...
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Meiotic pairing and double-strand break formation along the heteromorphic threespine stickleback sex chromosomes
Double-strand break repair during meiosis is normally achieved using the homologous chromosome as a repair template. Heteromorphic sex chromosomes...
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A novel DNA double-strand breaks biosensor based on fluorescence resonance energy transfer
Revealing the spatiotemporal behavior of DNA double-strand breaks (DSBs) is crucial for understanding the processes of DNA damage and repair....
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Time-resolved microfluidics unravels individual cellular fates during double-strand break repair
BackgroundDouble-strand break repair (DSBR) is a highly regulated process involving dozens of proteins acting in a defined order to repair a DNA...
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A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci
Immunofluorescent tagging of DNA double-strand break (DSB) markers, such as γ -H2AX and other DSB repair proteins, are powerful tools in understanding...
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Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Nonhomologous End Joining
This book chapter describes a plasmid-based reporter method, first described by Bennardo et al. (2008) that we use in our laboratory for determining...
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HLTF disrupts Cas9-DNA post-cleavage complexes to allow DNA break processing
The outcome of CRISPR-Cas-mediated genome modifications is dependent on DNA double-strand break (DSB) processing and repair pathway choice....
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Preliminary Study on 53BP1-Mediated DNA Double-Strand Break Response in Spermatogonial Stem Cells
53BP1 mediates DNA repair process in somatic cells; however, the function of 53BP1 in germline stem cells still remains unclear. In the present...
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Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing
BackgroundDue to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to...
