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Survey of Enzymological Data on ODCase
OMP decarboxylase (ODCase) has been the subject of enzymological, structural, and theoretical studies for several decades, yet its highly proficient... -
Crystallographic Studies of Native and Mutant Orotidine 5′phosphate Decarboxylases
This review aims to use the results of an approach combining crystallographic structure analysis with mutational studies as a framework for the... -
What Has Theory and Crystallography Revealed About the Mechanism of Catalysis by Orotidine Monophosphate Decarboxylase?
In 1995, Wolfenden and Radzicka showed that orotidine 5′-monophosphate decarboxylase (ODCase) was the most proficient enzyme. Since then, the... -
Catalysis by Enzyme Conformational Change
An energy decomposition scheme is presented to elucidate the importance of the change of protein conformation substates to the reduction of... -
Insight into the Catalytic Mechanism of Orotidine 5′-phosphate Decarboxylase from Crystallography and Mutagenesis
With a half-time of 78 million years at room temperature, the spontaneous decarboxylation of orotidine 5′-phosphate (OMP) is among the slowest... -
Develo** Active Site Models of ODCase—from Large Quantum Models to a QM/MM Approach
The catalytic mechanism of orotidine monophosphate decarboxylase (ODCase) has been modeled using density functional theory with the B3LYP functional.... -
Optimization of biotransformation processes of Camarosporium laburnicola to improve production yields of potent telomerase activators
BackgroundTelomerase activators are promising agents for the healthy aging process and the treatment/prevention of short telomere-related and...
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Map** a sustainable approach: biosynthesis of lactobacilli-silver nanocomposites using whey-based medium for antimicrobial and bioactivity applications
This study explores a sustainable approach for synthesizing silver nanocomposites (AgNCs) with enhanced antimicrobial and bioactivity using safe Lactobacillus...
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Production of succinate with two CO2 fixation reactions from fatty acids in Cupriavidus necator H16
BackgroundBiotransformation of CO 2 into high-value-added carbon-based products is a promising process for reducing greenhouse gas emissions. To...
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Construction of lignan glycosides biosynthetic network in Escherichia coli using mutltienzyme modules
BackgroundDue to the complexity of the metabolic pathway network of active ingredients, precise targeted synthesis of any active ingredient on a...
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Natural dyes developed by microbial-nanosilver to produce antimicrobial and anticancer textiles
Develo** special textiles (for patients in hospitals for example) properties, special antimicrobial and anticancer, was the main objective of the...
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Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1
BackgroundIn this study, we isolated a cellulase-producing bacterium, Bacillus amyloliquefaciens strain elh, from rice peel. We employed two...
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Streamlining heterologous expression of top carbonic anhydrases in Escherichia coli: bioinformatic and experimental approaches
BackgroundCarbonic anhydrase (CA) enzymes facilitate the reversible hydration of CO 2 to bicarbonate ions and protons. Identifying efficient and...
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Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches
BackgroundAdvancing the engineering of photosynthesis-based prokaryotic cell factories is important for sustainable chemical production and requires...
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A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater
BackgroundPlastic is widely utilized in packaging, frameworks, and as coverings material. Its overconsumption and slow degradation, pose threats to...
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Rational construction of a high-quality and high-efficiency biosynthetic system and fermentation optimization for A82846B based on combinatorial strategies in Amycolatopsis orientalis
BackgroundOritavancin is a new generation of semi-synthetic glycopeptide antibiotics against Gram-positive bacteria, which served as the first and...
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Engineering strategies for enhanced 1′, 4′-trans-ABA diol production by Botrytis cinerea
BackgroundCurrently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants...
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‘Small volume—big problem’: culturing Yarrowia lipolytica in high-throughput micro-formats
With the current progress in the ‘design’ and ‘build’ stages of the ‘design-build-test-learn’ cycle, many synthetic biology projects become...
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Metabolic engineering of Saccharomyces cerevisiae for chelerythrine biosynthesis
BackgroundChelerythrine is an important alkaloid used in agriculture and medicine. However, its structural complexity and low abundance in nature...