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Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and...
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Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of...
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Tracking Movement in Cell Biology
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques...
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Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within...
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Spectral Imaging and Linear Unmixing in Light Microscopy
Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows...
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Higher Harmonic Generation Microscopy
Higher harmonic-generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to the interacted matters...
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Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges...
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Current Topics Regarding the Function of the Medial Temporal Lobe Memory System
The first clear insight that the medial temporal lobe of the human brain was in fact a system of anatomically connected structures that were...
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Engineering Fluorescent Proteins
Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores...
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Live Cell Spinning Disk Microscopy
In vivo microscopy of dynamic processes in cells and organisms requires very fast and sensitive acquisition methods. Confocal laser scanning...
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Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological...
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Embelin Mitigates Amyloid-β-Induced Neurotoxicity and Cognitive Impairment in Rats: Potential Therapeutic Implications for Alzheimer’s Disease
Alzheimer’s disease (AD) is a significant form of dementia. Embelin (EMB) is a natural compound with varied actions that could help prevent AD...
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FABP3 Induces Mitochondrial Autophagy to Promote Neuronal Cell Apoptosis in Brain Ischemia-Reperfusion Injury
This study elucidates the molecular mechanisms by which FABP3 regulates neuronal apoptosis via mitochondrial autophagy in the context of cerebral...
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Nitrogen-fixing bacteria promote growth and bioactive components accumulation of Astragalus mongholicus by regulating plant metabolism and rhizosphere microbiota
BackgroundThe excessive application of chemical fertilizers in the cultivation of Astragalus mongholicus Bunge results in a reduction in the quality...
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A nonoscillatory, millisecond-scale embedding of brain state provides insight into behavior
The most robust and reliable signatures of brain states are enriched in rhythms between 0.1 and 20 Hz. Here we address the possibility that the...
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COVID-19-Associated Cerebellitis: A Case Report and Rehabilitation Outcome
Introduction . The COVID-19 pandemic has brought attention to neurological complications, including cerebellitis, characterized by inflammation of the...
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Unlocking opioid neuropeptide dynamics with genetically encoded biosensors
Neuropeptides are ubiquitous in the nervous system. Research into neuropeptides has been limited by a lack of experimental tools that allow for the...
