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  1. Deconvolution Microscopy

    Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and...
    Jean-Baptiste Sibarita in Microscopy Techniques
    Chapter
  2. Spectral Imaging and Linear Unmixing in Light Microscopy

    Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows...
    Timo Zimmermann in Microscopy Techniques
    Chapter
  3. Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience

    Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of...
    Tetsuichi Wazawa, Masahiro Ueda in Microscopy Techniques
    Chapter
  4. Fluorescence Lifetime Imaging Microscopy (FLIM)

    Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within...
    Erik B. van Munster, Theodorus W. J. Gadella in Microscopy Techniques
    Chapter
  5. Higher Harmonic Generation Microscopy

    Higher harmonic-generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to the interacted matters...
    Chi-Kuang Sun in Microscopy Techniques
    Chapter
  6. Tracking Movement in Cell Biology

    This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques...
    Kota Miura in Microscopy Techniques
    Chapter
  7. Fluorescence Correlation Spectroscopy with Autofluorescent Proteins

    Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges...
    Tobias Kohl, Petra Schwille in Microscopy Techniques
    Chapter
  8. Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins

    Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological...
    Adriaan B. Houtsmuller in Microscopy Techniques
    Chapter
  9. Engineering Fluorescent Proteins

    Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores...
    Atsushi Miyawaki, Takeharu Nagai, Hideaki Mizuno in Microscopy Techniques
    Chapter
  10. Live Cell Spinning Disk Microscopy

    In vivo microscopy of dynamic processes in cells and organisms requires very fast and sensitive acquisition methods. Confocal laser scanning...
    Ralph Gräf, Jens Rietdorf, Timo Zimmermann in Microscopy Techniques
    Chapter
  11. An Overview of Tsetse Fly Repellents: Identification and Applications

    Tsetse flies are vectors of the parasite trypanosoma that cause the neglected tropical diseases human and animal African trypanosomosis....

    Olabimpe Y. Orubuloye, Njelembo J. Mbewe, ... Baldwyn Torto in Journal of Chemical Ecology
    Article Open access 08 July 2024
  12. Nucleoside and non-nucleoside reverse transcriptase inhibitor drugs (NRTIs and NNRTIs) are capable of binding Chandipura virus polymerase protein (L) and inhibit virus replication

    Chandipura virus (CHPV) is an emerging pathogen of Indian subcontinent. It is a vector borne virus and belongs to Rhabdovirus family. In recent past...

    Dibyakanti Mandal, Deeksha Pandey, ... Manish Kumar in VirusDisease
    Article 08 July 2024
  13. Evaluation of vaccine candidates against Rhodococcus equi in BALB/c mice infection model: cellular and humoral immune responses

    Rhodococcus equi ( R. equi ) is a zoonotic opportunistic pathogen that mainly causes fatal lung and extrapulmonary abscesses in foals and...

    Lu Liu, Peng Cai, ... Hongqiong Zhao in BMC Microbiology
    Article Open access 08 July 2024
  14. Genome sequencing of SARS-Co-V-2 reveals mutations including F559I and V781D in S protein and LI123-124L in the nsp6 in 21K and 21L clades

    Contagious and virulent virus variants like B.1.1.529 have complicated the 2019 global COVID-19 pandemic from Wuhan, China. Omicron, with extensive...

    Mohamed G. Seadawy, Mohamed I. Marei, ... Ahmed R. Sofy in VirusDisease
    Article 08 July 2024
  15. VCP/p97-associated proteins are binders and debranching enzymes of K48–K63-branched ubiquitin chains

    Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding of branched Ub...

    Sven M. Lange, Matthew R. McFarland, ... Yogesh Kulathu in Nature Structural & Molecular Biology
    Article Open access 08 July 2024
  16. Evaluating the effects of temperature and agitation on biofilm formation of bacterial pathogens isolated from raw cow milk

    Objectives

    To study the effect of agitation and temperature on biofilm formation (cell aggregates embedded within a self-produced matrix) by...

    Meshari Alabdullatif in BMC Microbiology
    Article Open access 08 July 2024
  17. The Structure of the LysR-type Transcriptional Regulator, CysB, Bound to the Inducer, N-acetylserine

    In Escherichia coli and Salmonella typhimurium , cysteine biosynthesis requires the products of 20 or more cys genes co-ordinately regulated by CysB....

    Koen H. G. Verschueren, Eleanor J. Dodson, Anthony J. Wilkinson in European Biophysics Journal
    Article Open access 08 July 2024
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