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Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and... -
Spectral Imaging and Linear Unmixing in Light Microscopy
Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows... -
Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of... -
Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within... -
Higher Harmonic Generation Microscopy
Higher harmonic-generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to the interacted matters... -
Tracking Movement in Cell Biology
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques... -
Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges... -
Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological... -
Engineering Fluorescent Proteins
Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores... -
Live Cell Spinning Disk Microscopy
In vivo microscopy of dynamic processes in cells and organisms requires very fast and sensitive acquisition methods. Confocal laser scanning... -
An Overview of Tsetse Fly Repellents: Identification and Applications
Tsetse flies are vectors of the parasite trypanosoma that cause the neglected tropical diseases human and animal African trypanosomosis....
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Nucleoside and non-nucleoside reverse transcriptase inhibitor drugs (NRTIs and NNRTIs) are capable of binding Chandipura virus polymerase protein (L) and inhibit virus replication
Chandipura virus (CHPV) is an emerging pathogen of Indian subcontinent. It is a vector borne virus and belongs to Rhabdovirus family. In recent past...
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Evaluation of vaccine candidates against Rhodococcus equi in BALB/c mice infection model: cellular and humoral immune responses
Rhodococcus equi ( R. equi ) is a zoonotic opportunistic pathogen that mainly causes fatal lung and extrapulmonary abscesses in foals and...
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Genome sequencing of SARS-Co-V-2 reveals mutations including F559I and V781D in S protein and LI123-124L in the nsp6 in 21K and 21L clades
Contagious and virulent virus variants like B.1.1.529 have complicated the 2019 global COVID-19 pandemic from Wuhan, China. Omicron, with extensive...
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VCP/p97-associated proteins are binders and debranching enzymes of K48–K63-branched ubiquitin chains
Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding of branched Ub...
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Evaluating the effects of temperature and agitation on biofilm formation of bacterial pathogens isolated from raw cow milk
ObjectivesTo study the effect of agitation and temperature on biofilm formation (cell aggregates embedded within a self-produced matrix) by...
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The Structure of the LysR-type Transcriptional Regulator, CysB, Bound to the Inducer, N-acetylserine
In Escherichia coli and Salmonella typhimurium , cysteine biosynthesis requires the products of 20 or more cys genes co-ordinately regulated by CysB....