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Aging and mitochondrial dysfunction in the filamentous fungus Podospora anserina
In the filamentous fungus Podospora anserina, mitochondria play a paramount role in life span control and aging. During aging, these organelles... -
The human Werner Syndrome as a model system for aging
The aging process is one of the most challenging biological pathways to understand. Applications of model systems for aging have contributed... -
Aging and the programmed death phenomena
Biochemical mechanisms of the programmed death phenomena are considered at levels of unicellular organisms, mitochondria, cells, groups of cells, and... -
Deconvolution Microscopy
Since its introduction in 1983, deconvolution microscopy has become a key image-processing tool for visualizing the cellular structures of fixed and... -
Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of... -
Tracking Movement in Cell Biology
This article is an overview of techniques for measuring movement of proteins, vesicles and cells using digital image processing. Diverse techniques... -
Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within... -
Spectral Imaging and Linear Unmixing in Light Microscopy
Fluorescence microscopy is an essential tool for modern biological research. The wide range of available fluorophores and labeling techniques allows... -
Higher Harmonic Generation Microscopy
Higher harmonic-generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to the interacted matters... -
Energy metabolism, anti-oxidant defense and aging in Caenorhabditis elegans
Food restriction and impaired gene function by mutation or RNAi treatment can extend the lifespan of Caenorhabditis elegans considerably. In contrast... -
Do green plants age, and if so, how?
Time-dependent ageing-like processes in green plants are discussed and compared to gerontological changes in animals and other organisms. The... -
Conditional senescence in prokaryotes
Bacteria are immortal in the sense that their capacity for reproduction appears limitless as long as the environment supports growth. However, this... -
Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges... -
Yeast as a model for ageing and apoptosis research
Apoptosis is a form of programmed cell death with a crucial role in health and disease in metazoans. Recent findings demonstrate the existence of an... -
Mammalian and bird aging, oxygen radicals, and restricted feeding
In this chapter, the relationship of aging with oxidative stress is reviewed. Endogenous tissue antioxidants do not determine aging because they... -
Role of subcytotoxic stress in tissue ageing
Most commonly stress-induced premature senescence (SIPS) is defined as the long-term effects of subcytotoxic stress on proliferative cell types,... -
Mitochondria, metabolism, and aging in yeast
Quantitative and qualitative changes in metabolism take place when the lifespan is extended in yeast either by genetic or nutritional manipulation.... -
Engineering Fluorescent Proteins
Green fluorescent protein from the jellyfish Aequorea victora (GFP) and GFP-like proteins from Anthozoa species encode light-absorbing chromophores... -
Live Cell Spinning Disk Microscopy
In vivo microscopy of dynamic processes in cells and organisms requires very fast and sensitive acquisition methods. Confocal laser scanning... -
Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological...