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Structure, membrane topology and influence of cholesterol of the membrane proximal region: transmembrane helical anchor sequence of gp41 from HIV
During the first steps of HIV infection the Env subunit gp41 is thought to establish contact between the membranes and to be the main driver of...
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The large extracellular loop of CD63 interacts with gp41 of HIV-1 and is essential for establishing the virological synapse
Human immunodeficiency virus type 1 (HIV-1) persists lifelong in infected individuals and has evolved unique strategies in order to evade the immune...
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Antibody response against selected epitopes in the HIV-1 envelope gp41 ectodomain contributes to reduce viral burden in HIV-1 infected patients
The ectodomain of gp41 is the target of potent binding and neutralizing antibodies (NAbs) and is being explored in new strategies for antibody-based...
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Structural basis of the T4 bacteriophage primosome assembly and primer synthesis
The T4 bacteriophage gp41 helicase and gp61 primase assemble into a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication....
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Structures and immune recognition of Env trimers from two Asia prevalent HIV-1 CRFs
Structure-guided immunofocusing HIV-1 vaccine design entails a comprehensive understanding of Envs from diverse HIV-1 subtypes, including circulating...
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Intermediate conformations of CD4-bound HIV-1 Env heterotrimers
HIV-1 envelope (Env) exhibits distinct conformational changes in response to host receptor (CD4) engagement. Env, a trimer of gp120 and gp41...
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Comparing methods for immobilizing HIV-1 SOSIPs in ELISAs that evaluate antibody binding
Enzyme-linked immunosorbent assays (ELISAs) are used to evaluate binding of broadly neutralizing antibodies (bNAbs) and polyclonal sera to...
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Cryo-EM structures of HIV-1 trimer bound to CD4-mimetics BNM-III-170 and M48U1 adopt a CD4-bound open conformation
Human immunodeficiency virus-1 (HIV-1), the causative agent of AIDS, impacts millions of people. Entry into target cells is mediated by the HIV-1...
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Gp41 dynamically interacts with the TCR in the immune synapse and promotes early T cell activation
The HIV-1 glycoprotein gp41 critically mediates CD4 + T-cell infection by HIV-1 during viral entry, assembly, and release. Although multiple...
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Human CD4-binding site antibody elicited by polyvalent DNA prime-protein boost vaccine neutralizes cross-clade tier-2-HIV strains
The vaccine elicitation of HIV tier-2-neutralization antibodies has been a challenge. Here, we report the isolation and characterization of a...
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The influence of proline isomerization on potency and stability of anti-HIV antibody 10E8
Monoclonal antibody (mAb) 10E8 recognizes a highly conserved epitope on HIV and is capable of neutralizing > 95% of circulating viral isolates making...
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HIV-1 subtype A1, D, and recombinant proviral genome landscapes during long-term suppressive therapy
The primary obstacle to curing HIV-1 is a reservoir of CD4+ cells that contain stably integrated provirus. Previous studies characterizing the...
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Structure-function analyses reveal key molecular determinants of HIV-1 CRF01_AE resistance to the entry inhibitor temsavir
The HIV-1 entry inhibitor temsavir prevents the viral receptor CD4 (cluster of differentiation 4) from interacting with the envelope glycoprotein...
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Selection analyses of paired HIV-1 gag and gp41 sequences obtained before and after antiretroviral therapy
Most HIV-1-infected individuals with virological failure on a pharmacologically-boosted protease inhibitor (PI) regimen do not develop PI-resistance...
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Common evolutionary features of the envelope glycoprotein of HIV-1 in patients belonging to a transmission chain
The diversity of the HIV-1 envelope glycoproteins (Env) is largely a consequence of the pressure exerted by the adaptive immune response to...
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Molecular insights into antibody-mediated protection against the prototypic simian immunodeficiency virus
SIVmac239 infection of macaques is a favored model of human HIV infection. However, the SIVmac239 envelope (Env) trimer structure, glycan occupancy,...
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CoPoP liposomes displaying stabilized clade C HIV-1 Env elicit tier 2 multiclade neutralization in rabbits
One of the strategies towards an effective HIV-1 vaccine is to elicit broadly neutralizing antibody responses that target the high HIV-1 Env...
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Directing HIV-1 for degradation by non-target cells, using bi-specific single-chain llama antibodies
While vaccination against HIV-1 has been so far unsuccessful, recently broadly neutralizing antibodies (bNAbs) against HIV-1 envelope glycoprotein...
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Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell...
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A cybergenetic framework for engineering intein-mediated integral feedback control systems
The ability of biological systems to tightly regulate targeted variables, despite external and internal disturbances, is known as Robust Perfect...
